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Volume 21, Issue 1
Displaying 51-67 of 67 articles from this issue
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S AOMI, T HIRAYAMA, A TAKAZAWA, T ICHIHARA, R ABE, T TAKEMURA, A SHIIK ...1992 Volume 21 Issue 1 Pages 259-261
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSFrom January 1989 we used of SJM aortic valved graft prosthesis with non-crimped taperd section for the treatment of annuloaortic ectasia in 14 patients. Prosthesis was coated with 20% albumin and autoclaved for 15 minutes. In operation methods of coronary artery reattatchment direct anastomoses was 3 cases and in other cases separate dacron tube were interposed. Postoperative course were well and by post-operative CT and transesophagial echocardiography, the proximal and distal anastmoses were intact, all dacron coronary artery grafts were patent, and there were no pseudoanerysms at any sites in all 14 cases. This taperd section provided improved exposure of sewing ring and made coronary artery reattachment more tightly. SJM composite valve graft is more beneficial prostesis for the treatment of annuloaortic ectasia.View full abstractDownload PDF (3293K) -
T. SAKAMOTO, K. KATAMI, Y. OKUDA, S. YAMANOUCHI, T. KUMADA1992 Volume 21 Issue 1 Pages 262-267
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSWe developed an in vivo method to evaluate biocompatibility of small-caliber vascular grafts. Expanded polytetrafluoro-ethylene (EPTFE, 2 and 3mm internal diameter) and other vascular prosthetic materials (2mm internal diameter) were placed in the carotid arteries of normal male rabbits, using a sleeve (end in end) anastomosis, where a cut end of the artery is everted over an external splint, inserted into the graft tube, and then fixed with a circumferential ligature. At early times after the substitution, blood- and tissue-compatibility of the materials was determined by scanning electron and light microscope examinations. Thrombus deposition at 5-120min, patency rate at 1-7 days, and healing characteristics at 4-8 weeks after the substitution of EPTFE were found to be comparable with our previous results obtained using the conventional (end to end) suture method. The relative order of patency rate on the tested materials was shown as follows: EPTFE≅porous polyethylene>polyethylene>polyurethane. These results indicate that the rabbit sleeve anastomosis method is easy to perform, reproducible and useful in the in vivo screening of small-caliber vascular grafts for their short-term thrombogenicity and subsequent healing properties.View full abstractDownload PDF (6803K) -
Y. EZAKI, Y. TAKANO, K. NISHIHARA, M. TAKAHASHI, T. KATAKURA1992 Volume 21 Issue 1 Pages 268-274
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSWe studied the effect of the high porous vascular graft (porosity 2000-3200ml/cm2/min, 3mm I.D. 3cm in length and 4-5mm I.D. 7-8cm in length) coated with plasmin treated fibrin (PF) on healing and patency by implanting in the abdominal aorta of rabbits and carotid artery of dogs. The graft coated with PF matrix showed good handling and non oozing. We recognized the excellent healing of PF-coated prosthesis which were covered with intima without hyperplasia and stenosis in the rabbits. In addition, in the dogs, good patency were observed on the PF coated graft. These results suggest that it is possible to develope the high porous vascular prosthesis which will ensure non oozing and antithrombogenicity and histocompatibility.View full abstractDownload PDF (7325K) -
Y. NAKAGAWA, K. OTA, T. KAWAI, T. OSHIMA, S. TERAOKA, T. AGISHI1992 Volume 21 Issue 1 Pages 275-280
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSA new poly (ether-urethane) graft incorporated- with knitted polyester fibers (PE-PEUG) has been developed by our group. Although this graft has some advantages such as minimal bleeding from puncture site and durability to repeated puncture, it has become clear that it also shows a high stress at the time of puncturing and passing of the suture needle. To overcome these problems, a modification of the density and the thickness of the polyester fibers been done. The mechanical strength tests revealed that the improved PE-PEUG has enough tensile strength and low stress for the puncture and suture needle passing. The inner and external surface of the graft is coated with gelatin to improve patency rate and to facilitate adhision to the surrounding tissue. It is concluded that the coating of gelatin is effective to facilitate endotherialization at the anastomotic sites, although our observation is too short to draw any definitive conclusion.View full abstractDownload PDF (6242K) -
K. KANDA, S. SATOH, S. NIU, T. OKA, S. NAGAOKA, H. KURUMATANI, M. TAKA ...1992 Volume 21 Issue 1 Pages 281-284
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSIn this paper, we studied time-dependent porosity changes of two kinds of low porous vascular prostheses; one is a newly developed ultrafine polyester fiber (UFPF) graft, Toray Graft (TG, water porosity: 100ml/min/cm2, 120mmHg), and the other a conventional graft, Cooley Low Porosity Woven Dacron Graft (CG, 50ml/min/cm2). Both grafts, 26mm in diameter and 5cm in length, were assembled into a model circuit filled with human heparinized blood (20% in hematocrit, donated from volunteers). Then, TG was compared with CG from the aspect of blood leakage through the interstices of the grafts. The volume of blood leakage were measured every minute for the first ten minutes and every 10 minute for the next thirty minutes. The results showed that TG exhibited significantly larger volume of leakage, 4.32±0.91 (ml/min/cm2, mean±SD, n=5), than CG. 1.61±0.48, for the first one minute (p<0.05). Two minute later, however, blood leakage in both groups decreased rapidly and no significant difference was noticed thereafter. It is concluded that TG could be applicable to vascular reconstruction even under extracorporeal circulation after suitable presealing treatment preventing the initial blood leakage.View full abstractDownload PDF (2189K) -
Y. NOISHIKI, Y. YAMANE, H. KAJIWARA, K. HOSHINO, M. ISHII, Y. ICHIKAWA ...1992 Volume 21 Issue 1 Pages 285-289
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSA highly porous fabric vascular prosthesis sealed with autologous adipose tissue fragments into the wall was evaluated in an animal study. A piece of subcutaneous adipose connective tissue approximately weighing 2g was minced with scissors and stirred into 20ml of saline, thereby creating a tissue suspension. A fabric vascular prosthesis was turned inside out. The suspension was poured into the closed prosthesis by repeated pressurized injections using a syringe. Then, the prosthesis was turned inside out again. Consequently, the tissue fragments were squeezed into the prosthesis wall and sealed all the pores of the prosthesis. The treated grafts were implanted into the thoracic descending aortae of 24 mongrel dogs. No bleeding was noticed with the graft during and after implantation. Neointima with complete endotheloalozation was observed in all the grafts implanted for more than 30 days. Twenty-four preclotted prostheses were used as the control. Although the chest wall was closed after confieming that the bleeding from the suture line and through the prosthesis had stopped, problems occurred after surgery in the control grafts. Five out of 24 control animals (21%) bled in the pleural cavity and died within 24 hours after implantation. Neointima formation in the control grafts was limited near anastomotic sites in all cases up to 39 days of obserzation period. These results indicated that the sealing method was an ideal procedure of fabric vascular prostheses for cardiac wall substitutes. The results from an animal experiment could be expected in human if the property of the adipose tissue from human has a similar ability.View full abstractDownload PDF (6953K) -
Y. NOISHIKI, Y YAMANE, H. KAJIWARA, K. HOSHINO, M. ISHII, Y. ICHIKAWA, ...1992 Volume 21 Issue 1 Pages 290-294
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSFabric vascular prostheses transplanted with autologous venous tissue fragments showed extremely rapid healing of neointima in our previous study. In this communication, stability of the neointima in long-term was evaluated in an animal study. Highly porous fabric vascular prostheses (water porosity: 4, 000) were implanted into the descending aortae of 35 dogs, and were explanted from 1 hour to 495 days after implantation. A highly porous graft with preclotting was used as the control after the sealing by the original preclotting technique in 25 dogs (1 hour to 783 days). The neointima with the transplantation of autologous venous tissue fragments showed no degenerative changes such as calcification nor ulceration in the neointima. Control prostheses showed also no degenerative changes, however, in 9 out of 25 cases, hemothorax or perigraft hematomae were noticed. From these observations, stability of the neointima of fabric graft with autologous tissue fragments transplantation was confirmed clearly in this animal study.View full abstractDownload PDF (6877K) -
Y. TOMIZAWA, Y. NOISHIKI, T. OKOSHI, H. KOYANAGI1992 Volume 21 Issue 1 Pages 295-298
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSHeterologous connective tissue tube with "built-in" polyester mesh was developed using a rabbit subcutaneous tissue and polyepoxy compound cross-linking method. To evaluate an early healing process of the graft, 6 grafts were implanted end-to-end in the position of the thoracic aorta of 6 dogs and evaluated at 1hr, 24hrs, 7, 14, 28 and 73 days after surgery. The developed graft was white and pliable. The handling characteristics was very good and no bleeding was observed from the suture holes. Macroscopically, the most of the inner surface of the explanted graft was white and shinny without irregularity at 73 days but small area of blood stain was observed. Microscopically, fibroblasts were observed in the adventitia site of the graft after 7 days. Capillaries penetrating to the luminal surface appeared at 29 days graft. Also bleeding was observed around the fabric. A layer of donor connective tissue was still observed at 73 days. No foreign body reaction was observed except around the mesh. It was concluded the graft had an excellent ability of healing and could be an arterial substitutes.View full abstractDownload PDF (6999K) -
T. KOMATSU, E. MATSUBUCHI, H. NISHIDE, E. TSUCHIDA, M. WATANABE, K. KO ...1992 Volume 21 Issue 1 Pages 299-303
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSIn vivo oxygen transporting capacity of totally synthetic artificial red cell (poly-liposome embedded lipid-heme) solution, which can bind and release molecular oxygen under physiological condition, was described. Exchange transfusion with the lipid-heme solution to six dogs weighing about 8kg was carried out. After the transfusion with 30ml/kg of the lipid-heme solution, the oxygen partial pressure of mixed venous blood increased from 30 to 50mmHg. Oxygen consumption by the lipid-heme was calculated to be 15ml/min (20% of total oxygen transport), which is corresponding to the lipid-heme concentration in the blood stream. Circulation half-life time in the blood stream was about 11hr. for the lipid-heme and about 26hr. for the liposome. Oxidation rate of the lipid-heme (met-formation) in the blood stream was slower than that of in vitro: only 6% of the lipid-heme was oxidized at 11hr. after the injection.View full abstractDownload PDF (588K) -
A. USUBA, M. MIYAZAWA, J. MIURA, Y. ENDOH, H. INOUE, R. MOTOKI, K. SAK ...1992 Volume 21 Issue 1 Pages 304-308
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSThe purpose of this study is to evaluate the hemodynamics and oxygen transport capacity of liposome-encapsulated hemoglobin, named Neo Red Cells (NRC). Endotracheal intubation was made in 8 adult mongrel dogs weighing 10.0-13.0kg under intravenous anesthesia. Blood was extracted from the right femoral artery and NRC in the same amount was transfused. After blood exchange, the hemodynamics were investigated by inserting a Swan-Ganz catheter in the animal and oxygen transport capacity was assessed by performing blood gas analysis from time to time. From 59% to 95% of blood was exchanged with NRC. The dogs were divided into two groups according to the percentage of NRC exchanged: group 1-59%-86%, group 2-88%-95%. In every animal the cardiac index (CI) was found to have increased and the total peripheral vascular resistance index (TPRI) decreased and the rate of change of each was higher as the blood-NRC exchange rate increased. The CI increase was due to the TPRI decrease. Low viscosity of NRC is presumed to be the cause of TPRI decrease, which alleviated the burden on the heart. The A-V difference of oxygen content increased due to the increase of the oxygen transport efficiency of NRC which is more than that of natural erythrocyte. Because the CI and the A-V difference of oxygen content increased, oxygen consumption increased. Our conclusion is that NRC is more effective than natural red cells not only hemodynamically but also as a transport of blood gas.View full abstractDownload PDF (697K) -
S. TOKUYAMA, K. MORIZAWA, H. MATSUMOTO, O. NAKACHI, E. TSUCHIDA, S. SE ...1992 Volume 21 Issue 1 Pages 309-312
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSArtificial Red Cell (ARC) was prepared by encapsulation of purified hemoglobin (Hb) with phospholipid mixtures, mainly polymerizable 1, 2-bis(2, 4-octadecadienoyl)-sn-3-phosphocholine (DODPC), and it was subsequently stabilized by polymerization. Concerning physical characteristics, particle diameter 0.2μm, [Hb] 7-10g/dl, osmotic pressure 300mOsm, oncotic pressure 25torr, viscosity 4cp. P50 was adjusted to 30-40torr. Concerning physical stability, ARC showed no change in particle size and size distribution and no leakage of Hb after repeating freeze-thawing treatment. In the blood compatible tests (in vitro), there was no difference between ARC and control (PBS) about blood coagulation time [TT, PT, APTT, platlet aggregation], red cell hemolysis and red cell aggregation.View full abstractDownload PDF (487K) -
N MATSUDA, K NAKAI, H ABE, T TAKAHASHI, H IKEDA, H SEKIGUCHI1992 Volume 21 Issue 1 Pages 313-317
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSTwo analytical methods; high-performance liquid chromatography (HPLC) for phospholipids detection and enzyme immunoassay (EIA) for blood group antigen detection, were developed for the purpose of quality control of stroma-free hemoglobin (SFH). By HPLC four major phospholipids were separated clearly. The minimum detection levels of phosphatidylserin, phosphatidylethanolamine, phosphatidylcholine and sphingomyelin were 0.1, 0.2, 0.03 and 0.2μg, respectively. EIA had good reproducibility and the minimum detection level of blood group antigen was 6ng/ml. the ultrafiltration method with a 100kd membrane removed stroma effectively, as shown the prepared SFH contained only 1.4μg/ml of phospholipids and 12ng/ml of antigen. The removal efficiency of the stroma by the BMM methods was similar to the ultrafiltration method, whereas SFH prepared by 36, 0000xg centrifugation method contained 4.0μg/ml of phospholipids and 0.2μg/ml of antigen. As most conventional assays can not measure these low level of stroma, it is indicated that HPLC and EIA are useful to evaluate stroma content in SFH.View full abstractDownload PDF (601K) -
K. NAKAI, N. MATSUDA, H. ABE, M. KOBAYASHI, H. IKEDA, K. MORIZAWA, O. ...1992 Volume 21 Issue 1 Pages 318-322
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSA new method to prepare stroma-free hemoglobins (SFH) by the filtration with a virus removal filter, BMM, was developed, and evaluated in comparison with several representative SFH preparation methods, 36, 000xg centrifugation followed by the filtration with a 0.22μm filter and ultrafiltration with a 100kd membrane. Both the BMM method and the ultrafiltration method were shown to remove more than 99.7% of phospholipids, and 99.99% of blood group antigen. On the other hand, the material prepared by centrifugation still contained much of stroma. Next, a circulatory BMM filtration system was constructed to treat a large volume, which was demonstrated to work well even though solutions having much stroma such as lysate was applied. The BMM filter was originally developed as a virus removal filter. Theses indicate the BMM method is very useful to prepare a stroma-free, virus-reduced hemoglobin solutions.View full abstractDownload PDF (647K) -
T. HORIUCHI, K. HASHIMOTO, Y. TADA, Y. OHTA, T. DOHI, M. UECHI, T. WAT ...1992 Volume 21 Issue 1 Pages 323-327
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSPHP containing UW components were evaluated as perfusates for normothermic preservation of the isolated dog kidney. Kidneys flushed out by Lactate Ringer solution were connected to the perfusion circuit which consists of a preservation box, a reservoir of perfusate, a membrane oxygenator and a driving unit. PHP containing 140mEg/l of Na+ and 4mEq/l of K+(PHP(E)), UW solution (UW) and UW components added PHP(E) were adjusted at pH 7.4 prior to use. During 12 hour perfusion at 37°C with perfusion pressure of 100mmHg, remarkable change in pH were observed in UW and PHP(E) while higher oxygen consumption was noted in PHP(E)+UW than in PHP(E). The histological findings showed moderate damages of tubular epithelial cells and containing normal glomelular structure in PHP(E)+UW. There was no edematous degeneration in both UW and PHP (E)+UW, however, it was seen in PHP(E) alone. It was suggested that components of UW solution have positive effects on normothermic machine perfusion with PHP(E) solution.View full abstractDownload PDF (2257K) -
Norihito OKUMURA, Tatsuo NAKAMURA, Yasuhiko SHIMIZU, Yoshito IKADA, Ka ...1992 Volume 21 Issue 1 Pages 328-333
Published: February 15, 1992
Released on J-STAGE: December 02, 2011
JOURNAL FREE ACCESSA comparison was made between synthetic bioabsorbable mesh and non-absorbable mesh for the repair of tracheal defects, focusing on the wound-healing process. Patch windows measuring 1×1cm were created in the cervical trachea in dogs, and repaired using polyglycolic acid (PGA) mesh grafts as a bioabsorbable mesh, or by Marlex® (Polypropylene; PP) mesh grafts as a non-absorbable mesh.
Macroscopically, there was no difference between the two mesh groups until 3 weeks after the operation, when both types of graft were completely covered with host tissue and appeared partially Iustrous. At 6 weeks in the PP-mesh group, the thickness of connective tissue over the prosthesis was remarkable, and after 9 weeks, constriction of the replaced area by scar-like tissue was evident, as compared with the PGA-mesh group, which showed no constriction.
Microscopically, at 6 weeks, epithelium covered the whole area of the mesh in both groups. However, in the PP-mesh group, flat and poorly differentiated cells occupied a large area of the mesh, and after 9 weeks the height of the epithelial cells in the PP-mesh group was obviously less than that in the PGA-mesh group. Furthermore in the PP-mesh group, non-ciliated cells occupied a larger area even at 15 weeks, and the submucous connective tissue layer was apparently thicker than that in the PGA-mesh group after 6 weeks.View full abstractDownload PDF (9520K) -
T. MAEDA, Y. KUBO, S. NAKAJIMA1992 Volume 21 Issue 1 Pages 334-339
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSThe cervical trachea of mongrel dogs was replaced by a porous tracheal prosthesis, which was constructed with our personally designed Teflon ® support and Marlex ® mesh. The prostheses were divided into two types: in the first type (B-1) the mesh was fixed outside the support, and in the second type (B-2) inside the support. From the study of the polyethylene mesh which was implanted subcutaneously under aseptic conditions, we found that collagen fibers and newly formed vessels were present, if the mesh pore size was larger than 292μ. Thus we used Marlex ® mesh with a pore size of about 300μ. Macro- and micro-scopic examinations of the prosthesis were performed on the fifteen cases of B-1 support dogs and the ten cases of B-2 support dogs which survived more than a period of four weeks after the reconstruction. The incidence of stenosis at the site of the anastomosis increased gradually to 64% after one year, and the incidence of granulation tissue overgrowth on the inner surface of the graft also increased in the B-1 type when compared to the B-2 type graft, but this could not be statistically verified. The complete epithelization of the inner surface of the graft was disclosed in the B-2 type graft after six months, and in the B-1 type graft after eighteen months.View full abstractDownload PDF (5803K) -
[in Japanese]1992 Volume 21 Issue 1 Pages 340-342
Published: February 15, 1992
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSDownload PDF (394K)