Nippon Eiyo Shokuryo Gakkaishi
Online ISSN : 1883-2849
Print ISSN : 0287-3516
ISSN-L : 0287-3516
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Volume 62, Issue 1
Displaying 1-3 of 3 articles from this issue
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Original Papers
  • Constituents and Antioxidative Activity of a Hot-Water Extract of Adzuki (Vigna angularis) Beans
    Yumiko Hori, Tomomi Murakoso, Motonori Fukumura, Kazuo Toriizuka, Yosh ...
    2008 Volume 62 Issue 1 Pages 3-11
    Published: 2008
    Released on J-STAGE: February 28, 2009
    DOIhttps://doi.org/10.4327/jsnfs.62.3
    JOURNAL FREE ACCESS
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    Adzuki beans, the seeds of Vigna angularis Ohwi et Ohashi (Leguminosae), have been used not only as a food but also as a diuretic and anti-inflammatory folk medicine, and for treatment of dropsy, in China and Japan. In the present study we conducted phytochemical analysis of a hot-water extract of adzuki beans, and tested the radical scavenging activity of isolates from the extract. Thirteen flavonoidal derivatives were isolated and characterized, including two new compounds, and it was revealed that both the extract and the isolates have strong radical scavenging activities in the DPPH assay.
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  • Oleic Acid-bound Albumin Induces Apoptosis in Cultured Proximal Tubule Cells
    Aki Ogawa, Kanae Asai, Yoko Yamashita, Chidue Kumashiro, Masaru Takena ...
    2008 Volume 62 Issue 1 Pages 13-17
    Published: 2008
    Released on J-STAGE: February 28, 2009
    DOIhttps://doi.org/10.4327/jsnfs.62.13
    JOURNAL FREE ACCESS
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    Proteinuria is thought to be a risk factor for chronic kidney disease. Albumin, a major component of proteinuria, is a carrier protein and forms complexes with free fatty acids (FFAs) in vivo. It is known that oleic acid (C18: 1) is one of the major FFAs that binds to albumin. In this study, we investigated the effects of oleic acid-bound albumin (OA-BSA) on the viability of a cultured renal proximal tubule (PT) cell line, mProx24. OA-BSA decreased the viability of PT cells, and the effect was inhibited by several antioxidants, including N-acetyl-L-cysteine, resveratrol, vitamin C and Trolox (vitamin E). Caspase-3 activity was increased in PT cells by addition of OA-BSA. These data suggest that OA-BSA induces apoptosis by increasing oxidative stress in PT cells.
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Research Note
  • Pigments Production on Nitrogen Sources by Monascus anka AHU 9085
    Mami Takahashi, Takashi Matsumoto, Hatsue Moritaka
    2008 Volume 62 Issue 1 Pages 19-23
    Published: 2008
    Released on J-STAGE: February 28, 2009
    DOIhttps://doi.org/10.4327/jsnfs.62.19
    JOURNAL FREE ACCESS
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    The effects of five nitrogen sources (peptone, L-aspartic acid, (NH4) 2SO4, monosodium glutamate, KNO3) on the production of pigments by Monascus anka AHU 9085 were investigated. Pigment production in the presence of a 0.3% nitrogen source was greater than that in the presence of a 0.03% nitrogen source, and this increase was greatest when 0.3% peptone was used to produce yellow pigments and red pigments. As for the amount of pigment production, peptone gave the best yield among the five nitrogen sources, and addition of 0.3% L-aspartic acid to 1.0% peptone promoted the increase further. The ratio of A500/A400 was the largest for the combination of 1.0% peptone and 0.3% monosodium glutamate. In the case of peptone with L-aspartic acid, the main pigments increased were assumed to be monascorubrin, rubropunctatin, monascin and ankaflavin by HPLC analysis.
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