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Volume 44 (1991)
- Issue 6 Pages 429-
- Issue 5 Pages 335-
- Issue 4 Pages 239-
- Issue 3 Pages 155-
- Issue 2 Pages 79-
- Issue 1 Pages 1-
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Volume 44, Issue 2
Displaying 1-9 of 9 articles from this issue
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Yasuo KISHINO1991 Volume 44 Issue 2 Pages 79-88
Published: December 19, 1991
Released on J-STAGE: February 22, 2010
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Kazuharu SUZUKI1991 Volume 44 Issue 2 Pages 89-104
Published: December 19, 1991
Released on J-STAGE: February 22, 2010
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Hironobu TANIGUCHI, Toshichika TAKITA, Kahoru NAKAMURA, Takashi HAYAKA ...1991 Volume 44 Issue 2 Pages 105-111
Published: December 19, 1991
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSWe studied the effect of administration of refined eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (each at 3%) on lipid metabolism in rats with dietary hypertriglyceridemia, and obtained the following results. Levels of triglyceride (TG), total cholesterol (TC) and phospholipid (PL) in the serum and livers were significantly lowered in the EPA and DHA groups. This indicates that there were no differential effects between EPA and DHA in lowering of serum TG and TC in animals with dietary hypertriglyceridemia. The compositions of fatty acids in each serum lipoprotein fraction and in TG and PL fractions in the liver were affected by dietary lipids. That is, the proportion of C20: 5 and C22: 5 was increased in the EPA group and also C22: 6 in the DHA group in comparison with those in the control group. It was suggested that the lowering of serum lipids by administration of EPA or DHA in rats with dietary hypertriglyceridemia might be related to the increased proportion of n-3 PUFA in serum lipoprotein and liver lipid fractions.View full abstractDownload PDF (1259K) -
Takafumi NORII, Hiroo SUZUKI1991 Volume 44 Issue 2 Pages 113-122
Published: December 19, 1991
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSEffects of amino acid supplementation on serum alkaline phosphatase (APase, E.C. 3. 1. 3. 1) activity and zinc concentration in tissues were studied by feeding rats with a zinc-deficient diet containing 10% alkali-treated soy protein (AP) or 10% soy protein (DP). The growth of rats was completely depressed on the AP diet, but markedly improved by supplementation with either L-cystine or DL-methionine, whereas the growth of rats fed the DP diet was improved by supplementation with either L-lysine or methionine. Serum APase activity and femur zinc concentration in zinc-deficient rats were lowered by administration of the AP diet supplemented with either cystine or methionine, compared with those in zinc-deficient rats fed the AP diet alone. These values for zinc-deficient rats fed the DP diet supplemented with methionine were lower than those for zinc-deficient rats fed the DP diet alone. These results suggest that supplementation of diets with these amino acids may increase the zinc requirement of rats.View full abstractDownload PDF (1460K) -
Masayoshi IIO, Jun-ei KINJO, Toshihiro NOHARA1991 Volume 44 Issue 2 Pages 123-125
Published: December 19, 1991
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSEight isoflavones extracted from kudzu, Pueraria lobata (WILLD.) OHWI, were tested for their effect on glyoxalase I [E.C. 4. 4. 1. 5] (G-I) activity. One of the isoflavones, 8-C-glucosyl-7, 3′, 4′-trihydroxyisoflavone, (PG-1) strongly inhibited the action of the enzyme at 100μM. However, its inhibitory activity was reduced at lower concentrations in comparison with that of baicalein or quercetin. Some of the remaining isoflavones had a moderate inhibitory effect on G-I, whereas others had a weak effect. All eight isoflavones were also tested for their effect on the growth of HF, a hybridoma cell line. However, none of them inhibited the growth of the hybridoma.View full abstractDownload PDF (501K) -
Akihiko HASHIMOTO, Yoko TAKEUTI, Yuzo KAWAHARA, Kyoden YASUMOTO1991 Volume 44 Issue 2 Pages 127-132
Published: December 19, 1991
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSProteolytic enzyme (s) was partially purified from a crude extract of acetone-dried powder of ginger rhizome. The purified preparation gave a single main protein band of approximately 30, 000 Da. on SDS-polyacrylamide gel electrophoresis and hydrolyzed casein (proteinase activity) and type 1 collagen from bovine achilles tendon (collagenase activity). The collagenase activity of ginger was not affected by addition of EDTA, which it is known to be a potent inhibitor of collagenases from other sources, but was inhibited by HgCl2. The proteinase and collagenase activities of the ginger preparation decreased at a similar rate during heat-treatment. These results are interpreted to indicate that ginger rhizome contains a single enzyme which has both proteinase and collagenase activities.View full abstractDownload PDF (1062K) -
Aijiro YAMAMOTO, Kouzo ISHIBASHI, Akiko NOMURA1991 Volume 44 Issue 2 Pages 133-136
Published: December 19, 1991
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSWe determined the degree of gelatinization of rice grains by the β-amylase-pullulanase method to examine the effects of various treatments on the gelatinization of rice grains. Hot water-treated rice (abnormally cooked rice), which was kept at 75°C for 8h or at 75°C for 18h, showed a high degree of gelatinization, similar to that of rice cooked automatically in an electric cooker. However, the degree of gelatinization of abnormally cooked rice kept at 75°C for 4h was low, like that of aged rice prepared from automatically cooked rice by storage at 5°C for 96h. On the other hand, the degree of gelatinization of warm water-treated rice kept at 50°C for 72h or at 60°C for 72h, was lower than that of raw rice. The degree of gelatinization determined by the β-amylase-pullulanase method was in agreement with the results obtained by X-ray diffraction and the water uptake behavior of the rice grains.View full abstractDownload PDF (2299K) -
Katsuko NARUSE, Kumiyo YOKOYAMA, Sachiko TOKUHISA1991 Volume 44 Issue 2 Pages 137-142
Published: December 19, 1991
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSDeuterium-labeled compounds may be used as tracers in human metabolic studies. In this investigation, GC-MS-SIM analysis of D and L-Phe-d5, d3, Tyr-d4, d7 and Trp-d5 was performed. With the exception of Trp-d5 determination as its TMS derivative, the above deuterium-labeled amino acids were determined as their TFA-Me, TMS and BDMS derivatives. The fragmentions used for GC-MS-SIM are marked with a star in Fig. 1. No unfavorable elimination of deuterium was observed during derivatization (Fig. 2). This method was used for plasma samples after appropriate prior clean-up using cation-exchange (SCX) and non-polar (C18) minicolumns. Optical isomer separation was performed by HPLC using an enantio-selective column, CROWNPAK CR (+), which contains a chiral crown ether as a chiral selector (Fig. 3).View full abstractDownload PDF (865K) -
[in Japanese], [in Japanese]1991 Volume 44 Issue 2 Pages 143-146
Published: December 19, 1991
Released on J-STAGE: February 22, 2010
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