A method was investigated to artificially generate fruit core rot in Japanese pear by injecting a spore suspension through the calyx end of a fruit into the core using a syringe. When a spore suspension of
Phomopsis sp. was inoculated using 10
7 spores per fruit, a heavy type of fruit core rot was effectively generated. On the other hand, when a spore suspension of
Colletotricum sp. was inoculated at 10
5 spores per fruit, a mild type of fruit core rot was effectively generated.
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