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Volume 63 (2009)
- Issue 5.6 Pages 247-
- Issue 4 Pages 187-
- Issue 3 Pages 109-
- Issue 2 Pages 67-
- Issue 1 Pages 1-
Volume 63, Issue 2
Displaying 1-3 of 3 articles from this issue
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Original Article
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Fuminori NagaoArticle type: Original Article
2009 Volume 63 Issue 2 Pages 67-77
Published: 2009
Released on J-STAGE: June 15, 2010
JOURNAL FREE ACCESSAdministration of an exogenous IL-1 receptor antagonist (IL-1ra) is effective for reducing inflammatory reactions mediated by IL-1. In the present study, I transfected a plasmid containing IL-1ra into synovial cells using sonoporation and investigated the anti-inflammatory effects on inflammatory reactions induced by lipopolysaccharide (LPS) in vitro. IL-1ra-transfected synovial cells produced extracellular IL-1ra, with a remarkable increase seen when the cells were cultured with LPS for 48 hr. Interestingly, though treatment with LPS enhanced the release of IL-1β in the culture supernatant of IL-1ra-transfected cells, a significant decrease of PGE2 release was observed when the cells were cultured with LPS for 60 hr. These results indicate that IL-1ra has a valuable role in regulating inflammatory reactions by synovial cells and suggest that administration of IL-1ra by sonoporation is useful for controlling inflammation due to arthritis.View full abstractDownload PDF (976K) -
Toru Ishibe, Tetsuya Goto, Kentaro Kawata, Shigeru Kobayashi, Tetsu Ta ...Article type: Original Article
2009 Volume 63 Issue 2 Pages 78-86
Published: 2009
Released on J-STAGE: June 15, 2010
JOURNAL FREE ACCESSApolipoprotein E (ApoE) is a plasma protein that plays a major role in lipoprotein metabolism. Recent findings indicate that it is also involved in bone metabolism. ApoE mRNA is highly expressed during bone formation around titanium implants and adding ApoE to cultured osteoblasts enhances bone formation. However, the mechanism of ApoE expression in osteoblasts on titanium is not well understood. This study investigated the mechanism regulating ApoE mRNA expression in osteoblasts on titanium.
Rat or mouse primary osteoblasts or MC3T3-E1 cells were cultured on titanium, cover glasses, or culture dishes. The bone nodule formation on each substrate was measured, and the expression of ApoE and/or osteocalcin (OCN) was analyzed using reverse-transcription (RT) and real-time polymerase chain reaction (PCR). Bone nodules formed on titanium and culture dishes, but not on cover glasses. RT-PCR showed strong expression of ApoE in the cells on titanium and culture dishes, but not those on the cover glasses. Real-time PCR analysis indicated that on day 14 the percentage expression of ApoE and OCN by cells on the cover glasses relative to those on titanium was 18% and 29%, respectively. Although MC3T3-E1 cells did not express ApoE mRNA, adding ApoE or conditioned medium of cultured primary osteoblasts induced the expression of ApoE in MC3T3-E1 cells.
These findings suggest that primary osteoblasts express ApoE during bone formation, which indicates that ApoE is involved not only in lipoprotein metabolism but also in bone formation on biomaterials such as titanium.View full abstractDownload PDF (843K) -
Tomotaka Hayashi, Kou Matsuo, Mitsuhiro Yoshida, Min Zhang, Hiroshi Fu ...Article type: Original Article
2009 Volume 63 Issue 2 Pages 87-96
Published: 2009
Released on J-STAGE: June 15, 2010
JOURNAL FREE ACCESSβ-thymosins (TBs), isolated from various vertebrates and invertebrates, are highly conservative 5-kDa polar peptides. Thymosin β4 (TB4) is the most abundant peptide representing about 70% to 80% of the total TB content in mammalian tissues, while thymosin β10 (TB10) is a minority member of TBs in human, rat and mouse tissues. Recent studies have revealed that TBs are multifunctional peptides involved in cell migration, angiogenesis, wound healing, inflammation, morphogenesis and tumor metastasis. Nonetheless, there have been very few reports concerning TBs directly related to the fields of dental research or clinical dentistry. In this study, we investigated the effects of TB4 and TB10 on the healing process in tongue wounds, using 27 amino-acid partial synthetic peptides of those TBs.
After perforating rats' tongues using a punch biopsy instrument, the synthetic peptides were injected intraperitoneally into each rat at the time of perforation and every day thereafter for four days. Controls for the treatment received identical amounts of phosphate buffered saline in the same manner. Histological sections of Day2, Day3 and Day4 cases were processed, and stained with hematoxylin-eosin (H-E) and phosphotungstic acid hematoxylin (PTAH). The area of the wound (W) was histologically subdivided into tissue defect (D) area and granulation tissue (G) area by our definition, with reference to H-E and PTAH sections. Each area was calculated using computer-aided data analysis. The ratio of the D area divided by the total (T) area of the cross-section, D/T value (%), in the TB4- and TB10-treated groups was always significantly smaller than in controls. On the other hand, only for Day2 cases, G/T values in experimental groups were significantly higher than in controls. These results suggested that TBs may minimize tissue damage, probably by suppressing necrosis and/or apoptosis during the initial stage of the wound.View full abstractDownload PDF (2054K)
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