A reaction mixture was devised for the synthesis of N-acetylglucosamine-6-phosphate in the bovine parotid gland extract. The reaction products were identified qualitatively as the phosphate esters of glucosamine and acetylglucosamine. The presence of three enzymes in the parotid gland, hexokinase, acetate activating enzyme and glucosamine-6-phosphate N-acetylase has been suggested. Cysteine, a component of the earlier mixture, inhibited the production of N-acetylglucosamine-6-phosphate probably by inhibiting the acetylation of glucosamine-6-phosphate. Cysteine was found to inhibit the color reaction in the procedure of Morgan-Elson method for the detection of N-acetylglucosamine.

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The dental care status has been surveyed on the employees of 16 factories in Fukuoka City consisting of 563 males and 621 females ranged in age from 16 to 59 years. The subjects were divided into both sexes and subdivided into age ranges from 16 to 19, 20 to 29, 30 to 39, 40 to 49 and from 50 to 59, and on these groups the caries incidence and the rate of treated cases were observed. The results obtained were summarized as follows. 1. Percent Caries Incidence Percent incidence for the male groups ranged from 63.3 to 93.5, and that for the female groups ranged from 74.8 to 100.0. The incidence was lowest in 16 to 19 years age groups of both sexes, and rose higher with increasing age stages. There was found no appreciable sex difference. 2. Mean Decayed Teeth (DT Number) The mean number of decayed teeth present in the male groups ranged from 1.4 to 1.8, and that in the female groups from 2 2 to 2.9, indicating slightly greater caries incidence found in the female groups. Age difference in this respect was not so significant. 3. Mean Number of Missing Teeth (MT Number) The mean number of missing teeth for the male groups ranged from 0.1 to 5.3, and that for the female groups from 0.2 to 5.4. The number tended to increase with increasing age stages. Thers was no significant difference between sexes. 4. Mean Number of Filled Teeth (FT Number) The mean count of filled teeth for the male groups ranged from 0.9 to 9.2, and for the female groups from 0.7 to 5.2. The number increased with increasing age stages, There was no significant difference between sexes in this respect. 5. Mean Number of DMFT The mean count of decayed, missing, and filled teeth for the male groups ranged from 2.6 to 10.7, and that for the female groups from 3.0 to 12.2, indicating that the latter groups had more DMFT than the former. This number also showed increase with increasing age stages of both sexes. 6. Mean Number of RT (DMFT minus FT) The number for the male groups ranged from 1.7 to 2.7, and for the female from 2.4 to 4.3, indicating the greater number for the latter groups. This number showed slight increase with advancing age stages of subjects. 7. Percent Tooth Number Requiring Treatment The percentage decreasod for the male groups from 65.0 to 13.7, and for the female groups from 78.2 to 34.9, indicating decreasing rates with advancing age stages, and greater percentage for the female groups than for the male groups. 8. The present survey of dental care status revealed that it was worse in the younger generations of both sexes but especially of female sex.

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For the purpose of a collective study on the dental caries susceptibility in a population of similar age groups, it has been generally accepted that the salivary Lactobacillus count and Snyder test constituted the most valuable tools with high degree of reliability. Of the two methods, Snyder test was used in the present study to know the possible correlation between the data given by the test and the number of decayed teeth found in 73 college students of both sexes ranging in age from 18 to 20. The results obtained were summarized as follows. 1) Snyder test showed fairly high degree of correlation with the number of decayed tooth. The test was positive within 24 hours, in the individuals having decayed teeth, while it was negative in the caries-free individuals as late as after 72 hours. 2) That the correlation between Snyder test and the number of decayed teeth was positive could be confirmed by means of factor analysis. 3) The present study showed that the test would find wide application in the clinical use toward those age groups where the tooth caries are rampant. 4) Further studies are warranted on Snyder test to as certain as to whether or not it shows positive correlation with future incidence of tooth caries.

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Aminotransfersae activity in the rat's salivary glands preparation has been studied and comparison was made with that of the liver preparation. The results showed that the enzyme activity estimated on the basis of per gm. of wet weight of tissue was considerably higher in the salivary gland preparation than in the liver preparation. The pH optimum of the salivary gland enzyme was at 7.6. The enzyme fractions obtained at the concentrations between 1.7 and 2.5 M of ammonium salfate showed the highest activity.

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In the practice of surface anesthesia by the local anesthetics in the field of dental and oral surgery, an instant and deep penetration of the anesthetics is most desirable. But in some specific cases long anesthesia is not necessary. In this respect auther has studied the influence of hyaluronidase on the action of several local anesthetics using the corneal-reflex of rabbits. The local anesthetics were xylocaine, carbocaine, dibucaine and tetracaine. The results obtained were summarized as follows. When hyaluronidase was added to the local anesthetics, the corneal-reflex disapeared readily just like control but the duration of anesthesia shortened with compared to the controls. This shortening of anesthesia is surely owing to the action of Hyaluronidase which promotes absorption of local anesthetics. However, the appearance of anesthesia was not earlier than that of control. This is due to the concentration of anesthetics was too hight. In the experiment of tetracaine in relatively low concentration the anesthesia appeared faster than that of the control.

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The use of reimplantation of bone-chips by the treatment of various bone lesions defects in the alveolarprocess and jaw bone has had very healing of the wounds. But on these method, the most important problem is not always free from contaminating infection. Then we were studied bacteriologicaly accoding cases of various lesion 177 cases. 1) It may be clear from our study that aerobic foci are isolated to 54 cases from various oral lesion 65 cases with high recoverry rate of 87.07%. These included Staphylococcus, streptococcus, Gram-negative Bacillus, Gram-negative Dipplococcus and Candida. 2) It may be clear from our study that anaerobic foci are isolated to 35 cases from various oral lesion 52 cases with rate of 67.30%. These included Gram-positive Coccus, Bacillus, Actinomyces, Gramnegative Staphylococcus, Streptococcus or Bacillus. 3) Resistanes of these orgamismus to various antibiotics was in the decreasing order as follw : Penicillin>Tetracyclin, Chloramphenicol. 4) Bacterial examination of thetissue preparation were found many cases. Whereas in the preparation, these organismus were distributed abundantly in the periphery rather than in the bone.

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