Using mice (D. D. D. strain) and rats (Wistar strain), the author observed the following;
1) Intraperitoneal administration of Hemophilus pertussis vaccine (H-P-V) to mice and rats by single injection or by 20 serial injections in 1/20 divided dosis, led to increased histamine sensitivity, reaching the highest peak 4-5 days after the last injection, while the injection with mixed typhoid-paratyphoid vaccine, diphtheria vaccine or tuberculin, did not give similar result.
2) Histamine sensitivity induced by H-P-V in mice and rats was depressed by diphenhydramine or prednisolone given immediately before the histamine challenge, but it was not inhibited when given during the sensitizing period. On the contray, it was inhibited by vitamine P (Quercetin) given during the sensitizing period but was not inhibited when given immediately before the challenge injection.
3) Decarboxylase inhibitor (α-methyl-DOPA) did not inhibit the development of histamine sensitivity induced by H-P-V.
4) Injections of histamine 5mg daily for five days did not increase histamine death rate in rats on the sixth day.
5) Serum histaminase activity (determined by Kapeller-Adler method) proved decreased by H-P-V sensitization on the fifth day after vaccination.
6) Serum diamine oxidase activity (Ravin) was markedly increased by the injection of H-P-V but it was not increased by injection of Typhoid-paratyphoid vaccine or tuberculin.
7) The increase of serum diamine oxidase activity induced by H-P-V was not inhibited by prednisolone, α-methyl-DOPA or Quercetin.
8) Histamine sensitivity was induced by repeated injections of small doses of H-P-V in rats as mentioned already, however, serum diamine oxidase activity was not increased by such procedure.
9) Liver function tests (serum total protein, A/G ratio, alkaline phosphatase, GOT, GPT, cholin esterase activity) revealed no injurious effect of the administration of H-P-V.
10) Guinea pigs were bathed in plain water or 1g/l saline water at a temperature of 38-39°C for 15min. every day for 20 days. Histamine sensitivity of the lung was measured by a modified Friebel's apparatus. No definite change was observed by plain water bath but it showed an increase at the initial stage and a decrease at the end of the serial saline bathings. Serum histaminase activity showed a tendency to decrease at the initial stage and a recovery at the end of the serial saline bath. Serum diamine oxidase activity was gradually increased as the serial thermal baths were carried on.
11) Histamin sensitivity of the guinea pig was most markedly lowered by serial hydrogen sulfide (sulfur) water bathings for 2 weeks. Serum histaminase and also diamine oxidase activities also showed a marked increase by the serial sulfur water baths.
12) Rabbits were bathed in plain water, 1g/l NaCl, 1g/l Na₂SO₄ or 5mg/l H₂S waters at 39°C for 20min. daily for 2 weeks. Serum histaminase activity before each bath rose as the serial bathings of NaCl and Na₂SO₄ water were continued while it showed a tendency to fall by plain water or NaHCO₃ water baths.
In NaCl-bath group a marked fall in histaminase activity by the bath was observed in two weeks.
Diamine oxidase activity increased in the above mentioned 4 groups as the course of the serial thermal bathings were carried on, especially in NaCl and Na₂SO₄ water bathing groups.
13) Serum diamine oxidase activity was markedly increased by the serial thermal baths at Kusatsu spa, namely hydrogen sulfide containing strongly acid water bath, especially at the time of bath-dermatitis.

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Using radioactive isotope I¹³¹—NaI¹³¹ was mixed in 10% Na₂SO₃ solution, radio activity 100mc/ml, radio chemical purity 99.9%—, percutaneous absorption of iodide from bath water, peroral absorption after internal use of iodine containing mineral water, and the influence of absorbed iodine on the thyroid gland were studied in mice and rabbits.
Each mice was fixed in a small cage following the “bambootube” method of Masaji Seki, and bathed till the lower half of the body in the bath water at 37°-40°C, 20-30 minutes. NaI¹³¹ was added in the bath water to reach a radioactivity of 100-200μc/l. After the bath the surface of the animal body was washed out thoroughly with running water. Then the breast was cut open under anesthesia using chloroform or ether and blood was taken by heart puncture. The radioactivity (RA) of the blood was then measured by G-M counter or scintillation counter (well-typed).
Rabbit was bathed, fixed in a box, till the lower half of the body. The blood was taken from the ear vein. The radioactivity around the thyroid gland was measured by a survey meter.
For internal use of the waters, a thin polyvinyl tube with a diameter of 2mm was perorally inserted into the stomach of the mice. In the case of rabbit. Nelaton catheter was used.
To study function of the thyroid gland after serial oral administration of Mobara Mineral water, rabbits were given 5μc of I¹³¹. Then I¹³¹ uptake of the thyroid gland and plasma conversion rate after 24 hours were determined.
The investigations revealed the following results:
1. The longer was the duration of bath, the greater proved the amount of percutaneously absorbed iodine. Likewise, percutaneously absorbed iodine showed a tendency to increase as the temperature of bath water and as concentration of iodine in the bath water increased. It was recognized that iodide absorption continued still after the bath.
2. In relation to the influence of various co-existing ions upon the percutaneous absorption of I¹³¹, additon of sodium chloride, sodium sulfate, calcium chloride, or calcium sulfate at a concentration of 1g/l, showed an inhibition, while sodium bicarbonate, aluminium sulfate or pottasium bromide had a tendency to inhibit but not definitely. Especially, high concentration of sodium sulfate showed a strong inhibition, and in the case of sodium chloride similar finding was noted.
3. Concerning the effect of carbon dioxide and hydrogen sulfide an increase of percutaneous iodine absorption was demonstrated by the former.
4. After the serial daily bathings for 12 to 14 days in sodium sulfate solution, sodium chloride solution, or solution of pottasium iodide plus sodium chloride, respectively, an inhibition of percutaneous absorption of I¹³¹ was observed. As far as sodium chloride solution was concerned, the inhibition increased parallel with the length of the serial bathing period. On the other hand, after the serial bathings for 7 to 14 days in Kusatsu Hot Spring (H₂S-containing acid spring), an inhibition of percutaneous absorption of I¹³¹ was demonstrated, while after 21 days of the serial bathings an tendency of recovery in the percutaneous absorption was observed.
5. In adult rabbits thyroid uptake of iodine, in the amount corresponding to 1.8ml of bath water, was proved 48 hours after bathing for 30 minutes at 40°C in a bath water containing iodine at a concentration of 200μc/l.
6. Oral adminitstration of water containing I¹³¹ (0.25μc/l) resulted in a much more greater absorption than bathing in I¹³¹ added water (500μc/l). Oral administration of sodium bicarbonate or sodium chloride solution following the oral intake of I¹³¹ revealed no remarkable influence upon the absorption of I¹³¹
7. After oral administration of Mobara Mine

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