It was found that no effect of increase in dermal blood flow depended solely on HCO3- or CO32-, but on CO2 gas dissolved in water. The artificial CO2 bath was prepared with sodium bicarbonate and citric acid, mixing simply in plain water at 38-40°C. Thermal efficacy was confirmed by a rise in temperature of oral, finger tip and forehead respectively, and by a high transepidermal water loss (TWL) in consequence of increase in dermal blood flow, compared to a plain bathing. A remarkable effect in artificial CO2 bathing of 400-800ppm has been well-known clinically, however, a substantial increase in dermal blood flow has observed with artificial CO2-bathing of 59.8ppm or greater in this study, so that a CO2-bathing using the preparation would be popularized.
Increase in dermal blood flow by the artificial CO2-bathing was confirmed by means of a Thermocouple flow meter and by a Laser doppler velocimeter. The artificial CO2-bath was prepared with 50g tablet, made from sodium bicarbonate and succinic acid, putting simply in plain water at 38°C. Dermal blood flow was increased nearly 5-fold by the simple bathing, and was further enhanced 1.3-fold by the artificial CO2-bathing. It has been definitely shown by the artificial CO2-bathing that an increase in oral, finger tip, and forehead temperature and transepidermal water loss is significant compared to the plain bathing, so that the thermal effect equivalent to carbon-dioxated spring will be obtained.
Physiological effect of sera obtained from an electrically charged human body on giant axons of squid (Doryteuthis bleekeri) was examined by observing action potentials when the axons were extracellularly perfused with a 2:1 mixture solution of artificial sea water (ASW) and sera. Three kinds of sera were prepared; the one was obtained from a negatively-charged human body and the others were from a positively-charged and a non-electrically charged (or normal) human bodies. Amplitudes of action potentials in axons immersed in ASW containing the sera obtained from a negatively-charged human body first increased with time after the onset of the external perfusion. On the other hand, the amplitudes of action potentials in axons bathed in ASW containing the sera obtained from a positively-charged or a non-electrically charged human body did not increase but became reduced with time after the onset of the perfusion. These suggest that some facters involved in sera obtained from a negatively-charged human body are responsible for improving the function to generate nerve excitation. One of the facters may be increased number of electron charges involved in the sera from a negatively-charged human body since the flocculation number, which has been known to be inversely proportional to the number of electronic charges in the specimen, was experimentally confirmed to be relatively low in the sera from a negatively-charged human body.