All issues
Volume 48 (2009)
- Issue 6 Pages 353-
- Issue 5 Pages 257-
- Issue 4 Pages 159-
- Issue 3 Pages 91-
- Issue 2 Pages 47-
- Issue 1 Pages 1-
Volume 48, Issue 3
Displaying 1-12 of 12 articles from this issue
- |<
- <
- 1
- >
- >|
Original Articles
-
Yusuke MATSUURA, Rie URABE, Seiji KAGAMI, Toshinori KAWAGOE, Naoyuki T ...2009 Volume 48 Issue 3 Pages 91-96
Published: 2009
Released on J-STAGE: August 25, 2009
JOURNAL FREE ACCESSObjective : We analyzed clinicopathological features of women with invasive cervical cancer (ICC) previously undergoing Papanicolaou (Pap) smears.
Study Design : Of 864 patients with cervical cancer seen from 1984 to 2006, we retrospectively analyzed cytopathological results and clinical findings in 70 who had undergone Pap tests within 3 years of treatment.
Results : Among 530 women diagnosed with ICC, 70 (13.2%) had Pap tests within 3 years before cancer was detected. Of the 70, 43 had squamous cell carcinoma (SCC) and 27 non-SCC. The rate of adenocarcinoma and early-stage ICC (stageIb orII) was statistically higher in those undergoing cancer screening. Of the 70, 56 (80%) had atypical bleeding and 40 (57%) visible cervical cancer. Of the 70, 21 had “abnormal” Pap test results and 49 (70%) “negative” cervical smear reports.
Conclusion : False-negative Pap test results occur in about 10% of tested populations and are frequently seen in patients with cervical adenocarcinoma. Liquid-based cytology (LBC) and human papillomavirus (HPV) testing are therefore recommended in cervical cancer screening, as is clinicophysical examination.View full abstractDownload PDF (549K) -
Kikuko HAMANAKA, Fumiaki TEZUKA, Maki TAKAHASHI, Kayo HATAKEYAMA, Kuni ...2009 Volume 48 Issue 3 Pages 97-102
Published: 2009
Released on J-STAGE: August 25, 2009
JOURNAL FREE ACCESSObjective : We discuss the cytological, histological, and immunostaining features of solid pseudopapillary tumors (SPT) without pancreatic cyst formation as they compare to endocrine tumors (ET).
Study Design : Cytological, histological, and immunohistochemical examinations were conducted on surgically resected SPT and ET.
Results : SPTs without cyst formation consisted of small uniformly sized and shaped epithelial cells having solid and pseudopapillary patterns with hypovascular arborizing stroma. Imprint cytology showed 1) numerous small epithelial cells distributed singly and in loose fragments with ill-defined cytoplasm, monomorphic nuclei, and finely granular, even chromatin, and 2) peculiarly shaped fibrillary myxoid stroma substances. Immunohistochemistry showed an SPT phenotype strongly expressing vimentin and α1-antichymotrypsin, but lacking CK/CAM5.2 and chromogranin A expression, unlike the ET phenotype. SPTs also demonstrated intranuclear β-catenin and cyclin D1 accumulation.
Conclusion : Cytological and immunostaining features of pancreatic SPT, being highly specific, are extremely useful in supporting SPT and excluding ET diagnosis.View full abstractDownload PDF (865K) -
Atsushi FURUHATA, Yasuaki HIROOKA, Toshiharu MATSUMOTO, Kazuhisa ISHI, ...2009 Volume 48 Issue 3 Pages 103-109
Published: 2009
Released on J-STAGE: August 25, 2009
JOURNAL FREE ACCESSObjectives : To standardize cytological bile-juice preparation, we tested several preservative fluids using brushed cells and studied fluid effects by examining morphological changes in nuclei.
Study Design : Brushed epithelial cells from 15 gall bladders resected for cholecystolithiasis were suspended in bile juice including an equal volume of different preservative fluids. After reaction times of 5 minutes and, 1, 4, 8, 24, and 48 hours, we stained precipitates with Papanicolaou stain. Preservative fluids used were physiological saline, 20% bovine serum albumin fluid, RPMI1640 medium, 50% ethanol, CytoRich fluid, and YM fluid. We then observed morphological changes and nuclei staining patterns.
Results : High rates of well-preserved nuclei were obtained up to 24h after brushed cells were suspended in bile juice including CytoRich fluid or YM fluid.
Conclusion : CytoRich and YM fluids are highly effective in minimizing morphological changes in brushed cell nuclei. Our results suggest that both fluids are also effective with conventional bile cytology specimens when added immediately to bile juice after collection.View full abstractDownload PDF (643K) -
—Usefulness of multi-sected and Diff-Quik-stained imprint smear—Shunichi KAI, Miho HORI, Hideki GOTO, Kenji KASHIMA, Tsutomu DAA, Naom ...2009 Volume 48 Issue 3 Pages 110-113
Published: 2009
Released on J-STAGE: August 25, 2009
JOURNAL FREE ACCESSObjective : We evaluated the accuracy and usefulness of intraoperative imprint smears stained with Diff-Quik for sentinel lymph nodes (SLN) in breast cancer and the difference in the metastatic rate influenced by tumor size and histological type.
Study Design : Intraoperative imprint cytology of SLN was conducted in 225 patients with breast cancer. SLNs were bisected (125 cases) or multisected (90 cases) and stained with Diff-Quik after cut surfaces were imprinted. We compared the cytodiagnoses of multisected and bisected samples to histological diagnoses.
Results : Specificity, sensitivity, and accuracy in multisected samples were 100%, while in bisected cases, specificity was 92.3%, sensitivity 78.9%, and accuracy 94.1%. No definite relationship was seen between metastatic rate, tumor size, and histological type, but the metastatic rate of invasive lobular carcinoma and micropapillary carcinoma was high, even in small lesions.
Conclusion : Multisected and Diff-Quik-stained imprint smears for intraoperative cytodiagnosis of SLN in breast cancer is fast, cheap, and useful.View full abstractDownload PDF (394K)
Clinical Articles
-
Yuichi KINOSHITA, Tadanori YAMAGUCHI, Ryouichi ARIMA, Keizou NAKAYAMA, ...2009 Volume 48 Issue 3 Pages 114-118
Published: 2009
Released on J-STAGE: December 16, 2010
JOURNAL FREE ACCESSBackground : We report a rare case of neuroendocrine carcinoma arising in the endometrium.
Case : A 66-year-old woman with abdominal pain was found in magnetic resonance imaging (MRI) to have a huge mass in the abdominal and pelvic cavity. Endocervical smears showed round tumor cells isolated or in weakly bound clusters. Immunocytochemically, tumor cells were positive for synaptophysin. Histologically, the tumor shows solid growth with necrosis and a high mitotic rate.
Conclusion : Few reports have been made on neuroendocrine tumor differentiation in primary endometrial cancer, and, more examination is needed.View full abstractDownload PDF (697K) -
Shigeru TSUCHIDA, Yoshimasa NAKAZATO, Harumi KAMIYAMA, Rieko HARAGUCHI ...2009 Volume 48 Issue 3 Pages 119-123
Published: 2009
Released on J-STAGE: December 16, 2010
JOURNAL FREE ACCESSBackground : Follicular dendritic cell (FDC) sarcoma, a rare neoplasm occurring nodally and extranodally, very rarely occurs as primary pulmonary sarcoma. We report imprint-cytology findings in such a case and discuss the difficulties of differential diagnosis.
Case : A 63-year-old Japanese woman with cough, sputum, and pneumonia was found in computed tomography (CT) to have a lower left hepatic-lobe mass 3 cm in diameter. Transbronchial endoscopy showed a polypoid left-lobe mass. Imprint-cytology smears from endoscopic biopsy showed a cohesive group of pleomorphic polygonal cells but few lymphocytes, suggesting possible poorly differentiated squamous cell carcinoma, which was excised. Microscopic examination showed the tumor to congist of oval to spindle-shaped cells in sheets and interlacing storiform fascicles. The excised specimen contained numerous small lymphocytes. Tumor cells showed widespread immunoreactivity to a CD21+CD35 cocktail and anti-FDC antibodies. These findings yielded a definitive diagnosis of FDC sarcoma.
Conclusions : A dual cell population of large tumor cells and a lymphoid component consisting mainly of lymphocytes and plasma cells is cytologically indicative of FDC sarcoma. Our case demonstrates, however, that FDC sarcoma should be included in a differential diagnosis of poorly differentiated lung cancer even in the absence of a dual cell population in cytological specimens.View full abstractDownload PDF (531K) -
Miya NAKAGAWA, Takuya MATSUOKA, Yoshie MIZOKAMI, Keiji TANOUE, Takihir ...2009 Volume 48 Issue 3 Pages 124-128
Published: 2009
Released on J-STAGE: December 16, 2010
JOURNAL FREE ACCESSBackground : Primary thymic adenocarcinoma is extremely rare. We report such a case based on cytological findings.
Case : A 47-year-old woman undergoing regular health screening was found in chest computed tomography (CT) to had an abnormal shadow of the mediastinum. The tumor, 5 cm in diameter, was observed radiologically in the superior mediastinum, suggesting thymoma. Thoracoscopic needle biopsy indicated, malignancy, and the tumor was resected. Imprint cytology showed tumor cells with an increased nuclear/cytoplasmic (N/C) ratio scattered and proliferated in sheets or palisades in a slightly necrotic background. Glandular structures were seen in disordered nuclear arrays. Irregularly shaped nuclei had coarse nuclear chromatin. Focally, the glandular structure had mild atypia. Histologically, tumor cells had nuclear atypia and a disordered proliferated tubular or papillary nuclear arrangement. Thymus tissue with a Hassall body was observed on the cancer periphery. Immunohistochemically, tumor cells were positive for CD5. The definitive diagnosis was adenocarcinoma of the thymus.
Conclusion : Even if mediastinal tumor cytology indicates a glandular structure with mild atypia, thymic adenocarcinoma must be considered when a glandular structure with marked nuclear atypia and disordered nuclear arrays is observed.View full abstractDownload PDF (674K) -
Ayako ARAKAWA, Moriyuki NAKAMA, Yukinori MURATA, Tsuyoshi ISHIDA2009 Volume 48 Issue 3 Pages 129-134
Published: 2009
Released on J-STAGE: December 16, 2010
JOURNAL FREE ACCESSBackground : We report a case of apocrine carcinoma, a relatively rare histological breast cancer type, that shows intracystic male breast growth.
Case : An 83-year-old man noticing a right-breast mass was found in ultrasonography to have a cystic lesion with a solid component. Cytological specimens were taken from mastectomy material by needle puncture from both cystic and solid tumor components. The cystic component contained sheet-like clusters of cells with round nuclei, prominent nucleoli, and abundant vacuolated cytoplasm in a background of scattered foamy histiocyte-like epithelial cells. The solid component contained cohesive clusters of atypical cells showing apocrine features such as large acidophilic granular cystoplasm and snout-like projections. The cytological diagnosis was apocrine carcinoma.
Conclusion : Apocrine features in breast cytology are usually findings of mastopathy but the possibility of apocrine carcinoma should be considered when loose cohesive clusters of apocrine cells and vacuolated cells having round nuclei and thick cytoplasms are found in cytological specimens.View full abstractDownload PDF (771K) -
Rieko NISHIMURA, Norihiro TERAMOTO, Akihiro KAGAWA, Tamami YAMAMOTO, T ...2009 Volume 48 Issue 3 Pages 135-139
Published: 2009
Released on J-STAGE: December 16, 2010
JOURNAL FREE ACCESSBackground : Cell blocks are known to be superior to smears in morphologic interpretation and use for special staining. Their complex preparation and difficulty in collecting sufficient cells, however, have slowed the dissemination of their use. We present a case of thyroid carcinoma in which the cell block was useful in a definitive diagnosis.
Case : A man in his 30s admitted for a neck swelling was found to have a 6.5 cm tumor indicated in the front of the neck that initial fine-needle aspiration cytology was potentially malignant. To ensure appropriate treatment, we had to distinguish among poorly differentiated carcinoma, follicular carcinoma, small-cell carcinoma, and malignant lymphoma. Because the tumor had invaded a large vein, making it dangerous to take a direct biopsy specimen, we conducted aspiration cytology again, making a cell block. Hematoxylin and eosin staining showed several focally follicular epithelial clusters. Individual tumor cells had high nuclear cytoplasmic ratios and neither nuclear grooves nor nuclear inclusion. Immunohistochemical staining showed thyroglobulin to be strongly positive and cytokeratin, vimentin, and thyroid transcription factor-1 to be positive. Specimens were negative for neuroendocrine markers synaptophysin and chromogranin A. A cell block of the aspirate indicated thyroid follicular carcinoma, which was confirmed one month after admission following total thyroidectomy with cervical lymph node dissection.
Conclusion : Cell block preparation proved useful in definitively diagnosing a case of thyroid carcinoma in which lesions such as in the cervical region made a direct biopsy difficult.View full abstractDownload PDF (790K)
Brief Notes
-
Noriko WATANABE, Masaya AOKI, Shigemichi HIROSE, Hiroharu ARAI2009 Volume 48 Issue 3 Pages 140-141
Published: 2009
Released on J-STAGE: December 16, 2010
JOURNAL FREE ACCESSWe report a case of large cell neuroendocrine carcinoma, which is rare and difficult to diagnose using cytology alone.
A 57-year-old woman suspected of having invasive endometrial carcinoma with peritoneal dissemination found at laparotomy underwent total hysterectomy and bilateral salpingo-oophorectomy. An enlarged residual tumor, massive pleural effusion, and ascites subsequently appeared, however, and the woman died three months later. Preoperative endometrial cytology showed clusters of atypical cells with large nuclei and few prominent nucleoli. Histology showed loosely arranged cohesive solid patterns. Immunohistology findings were positive for CD56, synaptophysin, and NSE. The possibility of large cell neuroendocrine carcinoma should thus be considered in the differential diagnosis of cytomorphologically poorly differentiated lesions.View full abstractDownload PDF (292K) -
Yoshiko NAKAYA, Seiichi TAGUCHI, Toshimasa IZUMI, Tamiko UMEI, Ei KAWA ...2009 Volume 48 Issue 3 Pages 142-143
Published: 2009
Released on J-STAGE: December 16, 2010
JOURNAL FREE ACCESSWe report a case of extramammary breast carcinoma of the axilla, diagnosed by (FNA) cytology. A 61-year-old woman with a left axillar mass was found in cytological specimens to have numerous ductal epithelial cells mixed with myoepithelial cells.
Three years later, cytology from the ipsilateral axillar lesion showed higher cellularity.
Papillary cell clusters consist of large atypical cells with coarse chromatin. The axillary tumor appeared to be extramammary breast carcinoma, confirmed by the prior diagnosis of the extrammary breast.View full abstractDownload PDF (329K) -
Jumpei KAWAMURA, Fumikazu KIMURA, Shingo KAMOSHIDA, Takashi OMURA, Sad ...2009 Volume 48 Issue 3 Pages 144-145
Published: 2009
Released on J-STAGE: December 16, 2010
JOURNAL FREE ACCESSWe report immunohistochemical mucin features in a case of intraductal papillary mucinous adenoma (IPMA). Tumor cells were positive for MUC2, CDX-2, MUC5AC, and human gastric mucin but negative for MUC1 and M-GGMC-1. Immunocytochemical mucin-marker analysis proved useful in IPMN subtyping.View full abstractDownload PDF (229K)
- |<
- <
- 1
- >
- >|