All issues
Volume 39 (2006)
- Issue 6 Pages 145-
- Issue 5 Pages 125-
- Issue 4 Pages 103-
- Issue 3 Pages 61-
- Issue 2 Pages 31-
- Issue 1 Pages 1-
Volume 39, Issue 4
Displaying 1-3 of 3 articles from this issue
- |<
- <
- 1
- >
- >|
TECHNICAL ADVANCEMENT
-
Emilie Flaberg, György Stuber, Laszlo Szekely2006 Volume 39 Issue 4 Pages 103-106
Published: 2006
Released on J-STAGE: August 30, 2006
Advance online publication: June 02, 2006JOURNAL FREE ACCESSImaging live cells using laser confocal microscopy requires the use of complex and rather cumbersome incubation chamber systems in order to maintain the correct physiological conditions. The volume of these chambers is in the range of a few hundred microliters. Here we present an easy and convenient alternative in the form of glass capillaries that accommodate volumes of 0.2-10 microliters. The capillaries can be loaded with both suspension and adherent cells. The loaded capillaries are taped on microscope slides and submerged into the immersion oil that covers the objective. The correct temperature is maintained using a thermostat-controlled objective heater. We demonstrate that using microlens enhanced rotating Nipkow disc based confocal illumination, in combination with cold CCD cameras, maximum resolution multicolor time lapse fluorescence images can be obtained from live cells. The images obtained are free from disturbing optical distortions. Imaging in submicroliter volumes allows for fluorescence visualization of very rare cell types isolated using flow or affinity sorting or obtained by fine needle biopsies.
View full abstractDownload PDF (530K)
REGULAR ARTICLE
-
Koichi Suzuki, Fumihiko Takeshita, Noboru Nakata, Norihisa Ishii, Masa ...2006 Volume 39 Issue 4 Pages 107-112
Published: 2006
Released on J-STAGE: August 30, 2006
Advance online publication: June 28, 2006JOURNAL FREE ACCESSMycobacteria have acquired an intracellular lifestyle within the macrophage, which is best exemplified by the enlarged infected histiocytes seen in lepromatous leprosy. To survive within the cell, mycobacteria must escape intracellular bactericidal mechanisms. In a study of Mycobacterium bovis Bacille Calmette-Guérin (M. bovis BCG) infection, it was shown that the host protein, CORO1A, also known as tryptophan aspartate-containing coat protein (TACO), accumulates on the phagosomal membrane, resulting in inhibition of phagosome-lysosome fusion, and thus augmenting intracellular survival. In this study, we show that CORO1A strongly localizes on the membrane of phagosomes that contain Mycobacterium leprae (M. leprae), where Toll-like receptor 2 was also visualized by immunostaining. When cultured macrophages were infected with M. leprae, CORO1A recruitment from the plasma membrane to the phagosomal membrane was observed. Moderate to strong CORO1A retention was observed in late lesions that contained foamy histiocytes, in which M. leprae were difficult to detect by acid-fast staining. These results suggest that components accumulating within the phagosome rather than viable bacilli are responsible for the retention of CORO1A, and that there is also a bactericidal mechanism in the macrophage that might counter the effects of CORO1A.
View full abstractDownload PDF (454K) -
Kiyokazu Morioka, Toshiyuki Matsuzaki, Kuniaki Takata2006 Volume 39 Issue 4 Pages 113-123
Published: 2006
Released on J-STAGE: August 30, 2006
Advance online publication: July 27, 2006JOURNAL FREE ACCESSThe combined effects of myosin II and actin enable muscle and nonmuscle cells to generate forces required for muscle contraction, cell division, cell migration, cellular morphological changes, the maintenance of cellular tension and polarity, and so on. However, except for the case of muscle contraction, the details are poorly understood. We focus on nonmuscle myosin and actin in the formation and maintenance of hair and skin, which include highly active processes in mammalian life with respect to the cellular proliferation, differentiation, and movement. The localization of nonmuscle myosin II and actin in neonatal rat dorsal skin, mystacial pad, hair follicles, and vibrissal follicles was studied by immunohistochemical technique to provide the basis for the elucidation of the roles of these proteins. Specificities of the antibodies were verified by using samples from the relevant tissues and subjecting them to immunoblotting test prior to morphological analyses. The myosin and actin were abundant and colocalized in the spinous and granular layers but scarce in the basal layer of the dorsal and mystacial epidermis. In hair and vibrissal follicles, nonmuscle myosin and actin were colocalized in the outer root sheath and some hair matrix cells adjoining dermal papillae. In contrast, most areas of the inner root sheath and hair matrix appeared to comprise very small amounts of myosin and actin. Hair shaft may comprise significant myosin during the course of its keratinization. These results suggest that the actin-myosin system plays a part in cell movement, differentiation, protection and other key functions of skin and hair cells.
View full abstractDownload PDF (5385K)
- |<
- <
- 1
- >
- >|