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Volume 4 (1971)
- Issue 4 Pages 191-
- Issue 3 Pages 111-
- Issue 2 Pages 65-
- Issue 1 Pages 1-
Volume 4, Issue 1
Displaying 1-5 of 5 articles from this issue
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KUNIO TAKANO, TOSHIO SUZUKI1971 Volume 4 Issue 1 Pages 1-10
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe localization of parotin, which has been believed to have hormonal properties, is demonstrated in bovine parotid gland by means of immuno-histochemical technique.
The bright specific fluorescence, showing the localization of parotin, was encountered in the cytoplasm of supra- and para-nuclear regions in the cells of the duct system, but was not seen in the myoepithelial cells situated at the basal part of the duct. While, there was no specific fluorescence in the cytoplasm of the acinar cells. This fact explained that the presence of parotin was not able to be proved in the cytoplasm of the acinar cells by the immuno-histochemical method. On the other hand, according to the Ogata's report (1939), the parotin produced in the acinar cells was secreted into the lumen of the acinus, and the major part of the secreted parotin was re-absorbed in the apical cytoplasm of the cells of the striated duct. The absorbed parotin was then supposed to remove toward the basal part of the cells and to be oozed out into the interstitial connective tissue. If parotin were taken into the lumen of the lymphatic or blood vessels in such a way as mentioned above, strong specific fluorescence would not be observed at all in the intercalated duct or in the excretory duct. Consequently, parotin may be produced by the duct cells, or little amount of the parotin secreted from the acinus may be condensed in the tubular portion, or the presumable precursor of parotin may be secreted by the acinar cells and transformed into the parotin as soon as the precursor is re-absorbed in the duct cells.View full abstractDownload PDF (5229K) -
KINZI YOSHIKAWA1971 Volume 4 Issue 1 Pages 11-32
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSOsteolathyrism experimentally induced in weanling rats was observed morphologically, enzymatic histochemically and 3H-thymidine autoradiographically in the skeletal and periodontal tissues.
Morphological changes in the epiphyseal plate were an elongation of the plate and disarrangement of the cartilage cells, especially of the hypertrophic cartilage cell layer in the primary stage, and a shortening of the epiphyseal plate in the advanced stage. Histochemical activities for hydrolytic and oxidative enzymes were rather decreasing, but varying in the primary stage, while they were prominently low in the advanced stage.
Exostotic bone formation markedly appeared in the periostal hyperplastic region of active muscular traction. The hyperplastic periosteum was divided into the outer fibrous and inner osteogenic layers, where the enzymatic activities were found different to each other. Autoradiographic labelling in the normal and in initial stages appeared mainly in preosteoblasts of the inner layer and elongated fibroblasts of the outer layer, while active labelling was confined to the preosteoblasts in the advanced stage.
In tooth supporting structures, osteolathyritic changes were prominent in the periodontium, i.e., repeated development of palisade arranged connective tissue cells and eosinophilic amorphous substance. The periodontium, which revealed marked morphological changes, showed low or negative enzymatic ctivities. Mean percentage of 3H-thymidine labelled cells in the periodontium differed in the root apical, the alveolar crest and the intermediated regions. A progressive decreased percentage of labelled cells was observed in the root apical region in the advanced stage.View full abstractDownload PDF (25337K) -
MITSUAKI KODAMA1971 Volume 4 Issue 1 Pages 33-51
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe uridine diphosphate glucose-glycogen glucosyltransferase and phosphorylase activities in blood and bone marrow cells on smears were histochemically demonstrated by use of the improved techniques of Takeuchi's methods. To prevent destruction of cell structure and to keep the enzyme activity, the unfixed smears were immersed in the substrate mixture containing polyvinyl pyrrolidon of molecular weight 30, 000 at 37°C for 50 minutes in the glucosyltransferase demonstration and for 30 minutes in the phosphorylase technique. The substrate mixture was also improved by the fundamental experiments. Differentiation between native polysaccharide and the reaction products was performed by improved techniques.
The glucosyltransferase activity was demonstrated intensely in pseudoeosinophils (Neutrophils), moderately in eosinophils and weakly in thrombocytes, lymphocytes and monocytes in the peripheral blood. It was also demonstrated in these cells in the bone marrow, and it gradually decreased in the immature cells. Megakaryocytes contained this enzyme more richly. The phosphorylase activity was more intensely demonstrated in the blood and bone marrow cells in the same series mentioned above. They were also the same cells which may contain glycogen. The contents of these enzymes and glycogen in the cells of the same series were not always balanced, but the pseudoeosinophils were richer for glycogen metabolism. The reaction products for enzymes appeared in the cell matrices among specific granules in the cytoplasm of granulocytes. No reaction for the enzyme activities occurred in the cells of erythrocytic series in the normal rabbits. The index of cell number for positive reaction was counted for the series of white cells in the peripheral blood. It was greatest in pseudoeosinophils while it was about half in lymphocytes.View full abstractDownload PDF (9733K) -
Shohei YAMASHINA1971 Volume 4 Issue 1 Pages 52-56
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSGlucose oxidase in mold and rat kidney was demonstrated histochemically by means of a combined utilization of ferricyanide and lead ions. Brownish deposits of sulfurated lead ferrocyanide were found on the cell walls of the mold and in glomeruli and intertubular spaces of the kidney. It is presumed that the reducing activity of hydrogen peroxide generated during the enzymatic oxidation of glucose by glucose oxidase changes the ferricyanide to the ferrocyanide ion, which, reacting with the lead ion, makes whitish precipitates in the tissue.
Since the results indicate that hydrogen peroxide can be captured histochemically, the technique may be applicable to the histochemical detection of other kinds of oxidase if suitable substrate substitution are made. Furthermore, it may be expanded to electron microscopic histochemistry.View full abstractDownload PDF (2742K) -
OSAMI NADA, HSIEN-CHANG LAI1971 Volume 4 Issue 1 Pages 57-64
Published: 1971
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe fluorescence histochemical method of Falck et al., ('62) and an electron microscope were used for study on the uptake of exogenous noradrenaline (NA) by the adrenergic nerve terminal after reserpine treatment. The uptake of the NA was carried out during in situ application in the vas deferens of the rat pretreated with reserpine-iproniazid-pyrogallol by intraperitoneal administration. The NA was taken up in the adrenergic nerves, which showed a pronounced appearance of restituted NA fluorescence. Electron microscope studies revealed the reappearance of the small granular vesicles in restituted nerve terminals. Some terminals had almost the same appearance as compared with those seen in the normal tissue. These results indicate the occurrence of the uptake of exogenous NA into the small vesicles, in which the endogenous NA was depleted by reserpine pretreatment.View full abstractDownload PDF (7288K)
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