The homogeneity of
Aspergillus dipeptidase prepared according to the standard method established by us was ascertained by ultracentrifugation and some characteristic properties of the enzyme was further investigated.
Hydrolysis of various dipeptides by the purified dipetidase was tested in the presence of divalent metal ions such as Co
++ or Zn
++, and the characteristics of greatest interest may be enumerated as follows:
(1) The homogeneous dipeptidase requires Zn
++ for activation in the case of the hydrolysis of leucylglycine, leucylalanine leucylleucine, etc.
(2) The homogeneous dipeptidase requires Co
++ for activation in the case of the hydrolysis of glycylleucine, glycylleucine, glycylglycine, glycylphenylalanine, etc.
(3) In the case of the hydrolysis of alanylglycine, alanylleucine, valylglycine, etc., this enzyme does not require any metal ions.
抄録全体を表示