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Shinji UEDA, Tateki HAYASHI, Mitsuo NAMIKI
1986 年 50 巻 1 号 p.
1-7
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
The effect of ascorbic acid (AsA) on autoxidation of fat or linoleic acid was investigated in an oil-casein or cellulose mixture as the food model system; peroxides and free radical species were used as the criteria for autoxidation. While the addition of ascorbic acid suppressed autoxidation in systems of low moisture content (4%), the process was enhanced in systems with high water content (17%). In low-moisture systems, the addition of ascorbic acid at the initial stage of autoxidation produced ascorbic acid-derived free radicals which suppressed the oxidation, but had no effect at a later stage when the maximum peroxide value had been reached. This suggests that the suppression might be caused by the reaction of ascorbic acid with some intermediate product from the initial stage of autoxidation.
The enhancement of autoxidation by ascorbic acid in high-water content systems was suppressed neither by the addition of scavengers for the OH radical, Fe
2+, nor by chelating agents, which suggests non-involvement of active oxygen species, mediated or unmediated by the metal ion, in the effect of AsA.
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Makoto SHIMOSAKA, Yasuki FUKUDA, Kousaku MURATA, Akira KIMURA
1986 年 50 巻 1 号 p.
9-15
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
Two kilobase (2.0kb) DNA fragments bearing a gene (
pyrE) for orotate phosphoribosyltransferase (OPTase) were cloned into pBR322, from both
Escherichia coli K-12 C600 and its derivative, purine-sensitive mutant PS100 (caused, by a
pyrE mutation). Introduction of the resulting hybrid plasmids (designated as pNE24 with DNA from C600, and pNE31 with DNA from PS100) into
E. coli K-12 increased the amount of OPTase in the host cells to 30-80 fold. The PS100 cells with pNE31 overproduced a mutant-type OPTase with a decreased level of activity as compared to the wild-type OPTase. The purine-sensitive phenotype of PS100 was completely suppressed by introduction of either pNE24 or pNE31. In a reaction involving the conversion of orotate to orotidine-5'-monophosphate (OMP) by an
E. coli crude extract, the increased OPTase activity on the use of the hybrid plasmid, pNE24, was favorable for a high conversion rate.
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Hideaki YAMADA, Silvia Susana MIYAZAKI, Yoshikazu IZUMI
1986 年 50 巻 1 号 p.
17-21
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
A glycine-resistant mutant, GM2, showing improved L-serine production was derived from
Hyphomicrobium methylovorum KM 146. The mutant was found to have elevated methanol dehydrogenase and serine transhydroxymethylase activities. Using the mutant GM2, the optimum reaction conditions for serine production by resting cells were determined. The optimum concentrations of glycine, methanol and cells were 100mg/ml, 48mg/ml and 30 mg (as dry matter)/ml, respectively. The optimum buffer was 0.05 to 0.08 M Tris-HCl buffer, pH 10.2. Appropriate aeration was necessary. Methanol feeding and glycine feeding improved the L-serine production. Under the optimum conditions, 32-34 mg/ml of L-serine was produced.
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Mutsuo KANNO
1986 年 50 巻 1 号 p.
23-31
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
An extracellular amylase of
B. acidocaldarius, strain A-2, was purified as an electrophoretically homogeneous protein, and the properties of the purified enzyme were examined. This enzyme behaved like α-amylase (1, 4-α-D-glucan glucanohydrolase, EC 2.3.1.1). Its molecular weight,
Km value, and optimum reaction temperature and pH were 66, 000, 1.6mg starch/ml, and 70°C and 3.5, respectively. More than 70 and 90% of the initial activity remained after 30 min of incubation at pHs 2.0 (70°C) and 4.5 (90°C) in the absence of substrate. The thermal stability under acidic conditions is better than that of other
B. acidocaldarius α-amylases reported to date.
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Shiro SAITO, Yasunori NAGAMINE, Ichiyo OSHIMA, Tomoko KITA, Yoshihiro ...
1986 年 50 巻 1 号 p.
33-39
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
The serum levels of 18 constituents of 153 small cetaceans of both sexes from North-Western Pacific waters were measured. No sex differences were observed in any biochemical parameters in mature animals, but the serum levels of alkaline phosphatase (Alp), P, Na, and Cl were found to be age-related. The increase in serum Alp activity in younger animals seemed to originate from bone. Comparing the levels with those in humans there was (1) no difference in the levels of total protein, albumin, creatinine, total cholesterol, and γ-glutamyltranspeptidase, (2) a lower level of uric acid, and (3) higher levels of serum urea nitrogen, aspartate aminotransferase, alanine aminotransferase, lactic dehydrogenase, Alp, creatine kinase, Na, K, Cl, Ca, and P.
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Masanosuke TAKAGI, Hiroshi HIGASHIOKA, Kiyoka TAMURA, Naofumi MORITA
1986 年 50 巻 1 号 p.
41-47
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
Effects of L-ascorbic acid (AsA) and dehydro-L-ascorbic acid (DHA) on the peroxidation of linoleic acid (LA) were studied in neutral buffer solutions, pH 7, containing alcohol. The peroxidation of LA was accelerated by AsA in a buffer containing 10% EtOH, but suppressed in buffers with 20% or more EtOH, whereas the peroxidation of LA was accelerated after the complete consumption of AsA. Upon the addition of DHA instead of AsA in the same system, the peroxidation was accelerated at all alcohol concentrations tested except 40% EtOH, but with increasing alcohol concentrations, DHA showed a small lag of the peroxidation in the early stages. The appearance of the reaction mixtures was different with different alcohol contents; with 10% EtOH, it was turbid, but with 20% EtOH, translucent, after the mixtures were shaken at 37°C. Superoxide dismutase (SOD) suppressed the peroxidation of LA catalyzed by AsA at the concentration of 20% EtOH, but not at 10%. SOD did not suppress the accelerated peroxidation of LA by DHA.
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Minoru AMEYAMA, Masatsugu NONOBE, Emiko SHINAGAWA, Kazunobu MATSUSHITA ...
1986 年 50 巻 1 号 p.
49-57
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
Purification and characterization of the quinoprotein D-glucose dehydrogenase (EC 1.1.99.17) from
Escherichia coli K-12 were carried out, and the purified enzyme was obtained as the apo-form. The enzyme was purified 1, 400-fold with an overall yield of 28% from the membrane fraction of the organism by a procedure involving solubilization of the enzyme with Triton X-100, ion exchange chromatographies and gel filtration. The homogeneity of the enzyme was confirmed by SDS-polyacrylamide gel electrophoresis and analytical ultracentrifugation, and its molecular weight was estimated to be 88, 000. The purified enzyme itself had no enzyme activity, but it was markedly activated on the addition of pyrroloquinoline quinone in the presence of magnesium ions. The enzyme showed a broad substrate specificity for various monosaccharides including D-glucose, D-fucose, o-galactose, o-mannose and D-xylose. Oxidation of substrates proceeded rapidly at fairly acidic pH with ubiquinone, which may be the intrinsic electron acceptor of the organism. EDTA,
p-benzoquinone, citrate and semicarbazide showed inhibitory effects on the enzyme activity. The stability as to pH and heating was increased when the enzyme was converted to the holo-enzyme.
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Masaaki HIROSE, Hideo OE, Etsushiro Doi
1986 年 50 巻 1 号 p.
59-64
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
It was found that thiol reagents such as 2-mercaptoethanol induce the gelation of egg white at low temperatures that do not induce thermal denaturation. The thiol-dependent gelation was highly dependent on pH. Analyses by polyacrylamide gel electrophoresis revealed that gel matrices formed at pH 7-9 are mainly composed of conalbumin. In addition, purified conalbumin formed a fine gel by incubation with 2-mercaptoethanol at 35°C, and the gelation of both conalbumin and egg white was strongly inhibited by saturating the two iron-binding sites of conalbumin. Therefore, we concluded that conalbumin is a major component in the thiol-dependent gelation of egg white.
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Seiichi TANIDA, Toru HASEGAWA, Hisayoshi OKAZAKI
1986 年 50 巻 1 号 p.
65-70
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
Swimming behavior in
Tetrahymena pyriformis W was affected by calcium-function modulating compounds. Calmodulin antagonists such as phenothiazines, felodipine, clomipramine, dibucaine and W-7 induced an altered pattern of swimming: many cells swam backwards with active rotation. Normal behavior was restored after a few more minutes of incubation. The change in swimming behavior induced by the antagonists was supressed by adding EGTA. Among the calcium channel blockers tested, cinnarizine and nicardipine produced an effect similar to that of the calmodulin antagonists. A calcium ionophore, A23187, also induced the same pattern of swimming in
Tetrahymena, but its effect was not suppressed by EGTA. The transient change in swimming behavior occurred in the presence of BaCl
2, but unlike the case of calmodulin antagonists or A23187, the phenomenon was negated by adding CaCl
2.
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Teruo MIYAZAWA, Tomoko ANDO, Takashi KANEDA
1986 年 50 巻 1 号 p.
71-78
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
Eighteen male guinea pigs were divided into two groups and fed for 2 weeks with a diet containing 284 ppm of vitamin C and 50 ppm of vitamin E. One group received autoxidized linseed oil (40mg/animal/day) three times during the feeding. Compared with the animals not given the oxidized oil, the body weight gains, liver ascorbic acid levels and α-tocopherol contents were significantly decreased and liver lipid peroxidation was greatly stimulated in the animals dosed with the oxidized oil. In the next experiment, forty male guinea pigs were divided into five groups; only one group received a diet containing 1000 ppm of vitamin C and 50 ppm of vitamin E without oxidized oil, and the other four groups received graded diets of 1000-3000 ppm of vitamin C and 50-200ppm of vitamin E for 35 days. To the latter four groups, the oxidized oil (24 mg/animal/day) was orally administered 12 times during the feeding period. The tocopherol contents of the kidney and lung were found to be lower in the animals fed with a 3000 ppm-vitamin C and 200 ppm-vitamin E diet than those fed with a 1000 ppm-vitamin C and 200 ppm-vitamin E diet. The lung ascorbic acid content was lower in animals fed with a 3000 ppm-vitamin C and 200 ppm-vitamin E diet than in those fed with a 3000 ppm-vitmin C and 50 ppm-vitamin E diet. The effect of dietary vitamins C and E on tissue lipid peroxidation was different among the individual organs. From the results obtained by chemiluminescence analyses, the lipid peroxidation caused in the liver, kidney and heart was recognized to be suppressed effectively when 200 ppm-vitamin E diets were given. Antioxidative synergism of vitamin E (200 ppm) and C (3000 ppm) was found in the liver and lung as estimated by the chemiluminescence.
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Yoshimitsu YAMAZAKI, Hidekatsu MAEDA
1986 年 50 巻 1 号 p.
79-87
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
Stereoisomers of 7-carboxy- and/or 7-methoxycarbonylbicyclo[2.2.1]heptan-2-ol, heptan-2-one, hept-5-en-2-ol, and hept-5-en-2-one were prepared as the racemic and enantiomerically pure forms. Gas chromatographic enantiomer separation of these compounds was investigated for their diastereomeric derivatives prepared with eight chiral reagents. Among them, (
R)-(-)-1-(1-naphthyl)ethyl isocyanate and (
R)-(+)-α-methylbenzylamine were the most effective reagents for the hydroxy esters and keto acids, respectively, from the point of complete resolution of the enantiomers on OV-1 capillary columns. The peak components were assigned including the absolute configuration.
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Takuhei KIMURA, Yasuro KAWABATA, Eiji SATO
1986 年 50 巻 1 号 p.
89-94
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
Alcaligenes sp. T501, which has the ability to produce L-malate from maleate at a concentration of 20% in a molar yield of 98.4%, was isolated from soil. Analysis of the reaction mixture components suggested that maleate was converted to L-malate
via fumarate through the action of maleate isomerase and fumarase. These two enzymes were constitutively found in the cells regardless of the carbon source in the medium. The enzyme reaction was significantly enhanced by adding 2-mercaptoethanol, but was not affected by glutathione or L-cysteine.
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Tohru FUSHIKI, Nami YAMAMOTO, Ichiro NAESHIRO, Kazuo IWAI
1986 年 50 巻 1 号 p.
95-100
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
The digestion process for α-lactalbumin was investigated and compared with that of ovalbumin using their antigenicity as an index. α-Lactalbumin did not lose its antigenicity by incubation even at high temperature or acidic pH levels. By measuring the residual antigen, α-lactalbumin is shown to be hydrolysed rapidly by pepsin (1:66) or pancreatin (1:25)
in vitro without any previous denaturation treatment. The α-lactalbumin antigen also rapidly disappeared in the gastrointestinal tracts of rats. One hour after administering 250 mg of whey protein which contained 42 mg of the α-lactalbumin antigen, the α-lactalbumin antigen remaining in the stomach was only 3.95±0.4mg. In the small intestine, a trace amount of the antigen was detected. In more distal parts of the tract, no antigen was found. Rapid gastric emptying was achieved by using α-lactalbumin in a 0.9% NaCl solution without carbohydrate and lipids. However, the antigen recovered from the small intestine remained at a trace level. Thus the capacity for α-lactalbumin digestion in the small intestine may be very large. In the previous examination, a significant amount of the intact ovalbumin antigen was detected in the stomach and small intestine. These results suggest that the digestion of α-lactalbumin measured by the disappearance of its antigen is significantly different from ovalbumin digestion.
抄録全体を表示
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Kenshiro FUJIMOTO, Hiroko OHMURA, Takashi KANEDA
1986 年 50 巻 1 号 p.
101-108
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
The biological antioxidant activities of bromophenols (5-bromo-3, 4-dihydroxy benzaldehyde and 5-bromo-3, 4-dihydroxy benzylalcohol), protocatechualdehyde and vanillyl octadecanamide were compared with α-tocopherol
in vitro and
in vivo. All of these antioxidants significantly inhibited the lipid peroxidation in microsomal suspensions induced by ascorbate/Fe(II) or NADPH. By removing the non-binding free antioxidants from the suspensions, the inhibitory activities markedly decreased in antioxidants other than α-tocopherol; however, considerable activity was still observed. The effects of orally administered antioxidants on the symptoms of vitamin E deficiency were examined using rats fed with a vitamin E-free diet and supplemented with oxidized oils. As a result and contrary to the effects
in vitro, all of the antioxidants were much less effective in liver. However, some of the vitamin E-deficient symptoms such as organic solvent-soluble fluorescent materials in the heart and lung, and oxidative hemolysis were significantly suppressed by some antioxidants.
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Ichiro YAMASHITA, Dai HIRATA, Makoto MACHIDA, Sakuzo FUKUI
1986 年 50 巻 1 号 p.
109-113
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
DNA coding for a secretable acid protease gene of
Saccharomycopsis fibuligera was isolated from a genomic DNA library of the organism.
Saccharomyces cerevisiae cells transformed with a plasmid carrying the cloned gene secreted protease having the same enzymatic properties as those of the
S. fibuligera protease. Southern blot analysis of genomic DNA from
S. fibuligera confirmed that the protease gene was derived from
S. fibuligera.
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Kazuki KANAZAWA, Masato NATAKE
1986 年 50 巻 1 号 p.
115-120
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
Secondary autoxidation products (SP) of [
14C-U]linoleic acid were administered orally to rats and the incorporation of SP into the body was investigated. The radioactivity of SP was incorporated into the liver after 12 hr to the extent of 1-2.5%. An attempt was then made to detect the major low molecular-weight components of SP, 9-oxononanoic acid (9-ONA) and hexanal, in the hepatic mitochondrial and microsomal lipids. The fraction containing SP could be separated from the neutral and phospholipids of the organelles and their peroxides by silica gel column chromatography. The specific activity of the thiobarbituric acid-reactive substances of the fraction approached the value of SP
per se, and with gas chromatographic-mass spectrometric analysis, 9-ONA and hexanal were identified in the fraction. These detected compounds could not be artifacts and, therefore, were originated from the SP orally administered.
抄録全体を表示
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Takashi UTAGAWA, Hirokazu MORISAWA, Shigeru YAMANAKA, Akihiro YAMAZAKI ...
1986 年 50 巻 1 号 p.
121-126
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
A potent antiviral agent, Virazole (1-β-D-ribofuranosyl-1, 2, 4-triazole-3-carboxamide), was synthesized from inosine and 1, 2, 4-triazole-3-carboxamide (TCA) by a two step reaction with purified purine nucleoside phosphoryiase (PNPase) of
Enterobacter aerogenes AJ 11125. The first step involves the production of ribose-1-phosphate (R-1-P) through the phosphorolysis of inosine. The second step involves the production of Virazole through the transribosyl reaction of R-1-P and TCA. The R-1-P produced from inosine was isolated by ion-exchange chromatography and used as a substrate for Virazole synthesis without crystallization. TCA (10 mM) was transformed to Virazole with a 75% yield (molar basis) in the presence of 50mM R-1-P. Enzymatically synthesized Virazole was isolated from a large scale reaction mixture and identified by physicochemical means. The
Km values of PNPase for TCA and hypoxanthine were 167 mM and 5.6 mM, respectively.
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Masaaki UCHIDA, Mitsuyoshi UEDA, Toshitsugu MATSUKI, Hirofumi OKADA, A ...
1986 年 50 巻 1 号 p.
127-134
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
Isocitrate lyase (EC 4.1.3.1) was purified from the peroxisome-containing particulate fraction of an alkane-grown yeast,
Candida tropicalis, which had a conspicuous number of peroxisomes. The properties of the isocitrate lyase, which was induced by alkanes and localized in peroxisomes, were compared with those of the constitutive enzyme purified from glucose-grown cells of the yeast, which contained only a few pre-existing peroxisomes. The molecular masses of both enzymes were estimated to be about 130, 000 daltons by gel-filtration chromatography, and they were both composed of two identical subunits of a molecular mass of 65, 000 daltons. Both enzymes showed similar peptide maps upon partial digestion with proteolytic enzymes and were indistinguishable immunochemically, although there was a slight difference in their amino acid compositions.
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Kousaku MURATA, Yoshiharu INOUE, Kunihiko WATANABE, Yasuki FUKUDA, Tos ...
1986 年 50 巻 1 号 p.
135-142
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
Glyoxalase II, that catalyzes the conversion of S-lactoylglutathione into lactate and glutathione, was isolated from the yeast,
Saccharomyces cerevisiae, and some properties of the enzyme were determined. The glyoxalase II was purified approximately 100 fold from a cell extract with a 20% activity yield. Estimation of the molecular weight of the enzyme by both gel filtration and SDS/polyacrylamide gel electrophoresis gave a value of 1.9×10
4. Among the various thiol esters tested, the enzyme hydrolyzed only S-lactoylglutathione with a
Km=70×10
-6M. The enzyme was most active at low pH (pH 3-4) and was stable even in 0.001 N HCl at 40°C for at least 15 min. The activity of the enzyme was inhibited by various thiol compounds such as glutathione and coenzyme A. Hemimercaptal, a non-enzymatic condensation product of methylglyoxal and glutathione, strongly inhibited the activity of the enzyme.
抄録全体を表示
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Minoru YOSHIDA, Eisaku TAKAHASHI, Takeshi UOZUMI, Teruhiko BEPPU
1986 年 50 巻 1 号 p.
143-149
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
During the screening for inhibitors of K88 antigen synthesis that suppress hemagglutination by
E. coli E68 NIH 457-71, we found
Streptomyces sp. No. 207 produced two active substances, one of which was identified as hygromycin A. Another new compound, named methoxyhygromycin, was very similar to hygromycin A but methoxyhygromycin had a methoxy group on the neoinosamine-2 moiety instead of the methylene dioxy group found in hygromycin A. Although hygromycin A and methoxyhygromycin had almost no effect on the growth of
E. coli E68 NIH 457-71, they inactivated the hemagglutination activity of
E. coli at low concentrations of 12.5 μg/ml and 25 μg/ml, respectively.
抄録全体を表示
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Takashi MISE, Tadahisa SHIMODA, Gunki FUNATSU
1986 年 50 巻 1 号 p.
151-155
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
We analyzed [
125I]iodotyrosyl residues in the B-chain of ricin D iodinated at pH 7.0 and 0°C for 60min in the presence and absence of lactose. Peptides containing such [
125I]iodotyrosyl residues were isolated from the subtilisin digest of iodinated B-chains by gel filtration on Sephadex G-25 followed by paper chromatography and paper electrophoresis. Their locations in the primary structure were assigned based on their amino acid composition.
In the absence of lactose, five tyrosyl residues were susceptible to iodonation, but their reactivities varied; Tyr-248 seemed to be the most reactive, followed by Tyr-148, Tyr-78 (or Tyr125), and Tyr-67; Tyr-176 was the least reactive. In the presence of lactose, however, Tyr-248 was completely protected from iodination, and the reactivity of Tyr-67 increased more than two-fold; that of Tyr-78 (or Tyr-125) decreased by about half.
From these and earlier results, we concluded that Tyr-248 exists in the high-affinity saccharide-binding site of the B-chain. Tyr-67 and Tyr-78 (or Tyr-125) may exist next to each other near the low-affinity saccharide-binding site of the B-chain, and their iodination may cause a loss of lactosebinding ability.
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Akihiko TOJO
1986 年 50 巻 1 号 p.
157-162
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
The structure of the subunit protein of the bipyramidal δ-endotoxin of
Bacillus thuringiensis kurstaki strain HD-1 was analyzed by limited hydrolysis with a gut juice protease (P-III) of the silkworm,
Bombyx mori, and by neutralization tests using subunit protein IgG. The molecular weight of the subunit protein was 130, 000 by SDS-polyacrylamide gel electrophoresis. Hydrolysis of the subunit protein by P-III produced a toxic protein with 60, 000 dalton (P-59) and nontoxic peptides. A preparation of subunit IgG was put through P-59-agarose affinity chromatography, and the adsorbed fraction (TR-IgG) was separated. The unadsorbed fraction was reapplied to a subunit protein-agarose affinity column, and the adsorbed fraction (FR-IgG) was obtained. TR-IgG was capable of reacting to both subunit protein and P-59, and neutralized the toxicity of subunit protein. FR-IgG reacted to subunit protein but not to P-59. The toxicity of subunit protein was not neutralized by FR-IgG. These results indicate that the subunit protein is composed of protease-resistant region with a molecular weight of about 60, 000 and a residual protease-sensitive nontoxic region, which was surmised to be involved in the construction of the crystalline inclusion. Purified P-59 appeared to be a mixture of two protein species with similar molecular weights but with different charges.
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Takeshi YASUMOTO, Daisuke YASUMURA, Yasushi OHIZUMI, Masami TAKAHASHI, ...
1986 年 50 巻 1 号 p.
163-167
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
Two species of xanthid crab,
Lophozozymus pictor and
Demania alcalai, known to cause fatal poisoning when ingested, were collected on southern Negros, Philippines. They were highly fatal by mouse assays. The toxin in both species was identified as palytoxin, 'a highly lethal toxin of zoanthids, by chromatographic and mass spectrometric analyses. The toxin was found in all tissues but the concentrations were higher in the gills, viscera, and eggs.
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Soichi ARAI, Michiko WATANABE
1986 年 50 巻 1 号 p.
169-175
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
We propose a new process for freeze texturing using
Erwinia ananas cells as heterogeneous ice nuclei. The bacterial cells, when added to isotropic aqueous dispersions or hydrogels of proteins and polysaccharides, converted the bulk water into directional ice crystals at a subzero temperature of not lower than -5°C, with formation of anisotropically textured products. Raw egg white, bovine blood, soybean curd, milk curd, aqueous dispersions or slurries of soybean protein isolate, hydrogel of agar, corn starch paste, and hydrogels of glucomannan and calcium-bridged glucomannan were used as materials to be textured. These were each mixed with a small mass of the bacterial cells and slowly cooled to -5°C in an air bath. The frozen materials were vacuum-dried before setting the resulting textures by steaming for 2min at 1 atm if necessary, forming flake-like textures. Slicing the textured products at right angles to the lamellas of the flakes gave fibrous products.
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Teruyuki SAKAI, Kenji MORI
1986 年 50 巻 1 号 p.
177-183
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
(4
E, 8
E)-4, 8-Dimethyl-4, 8-decadien-10-olide
1 (ferrulactone I) and (3
Z, 11
S)-3-dodecen-llolide
2 (ferrulactone II), the macrolide pheromones of the rusty grain beetle, were synthesized starting from (2
E, 6
E)-farnesol and ethyl (
S)-3-hydroxybutanoate, respectively.
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Yukiho YAMAOKA, Osamu TAKIMURA
1986 年 50 巻 1 号 p.
185-186
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Yoshihiro NISHIDA, Masao KONNO, Hiroshi OHRUI, Hiroshi MEGURO
1986 年 50 巻 1 号 p.
187-189
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Yoshihiro NISHIDA, Masao KONNO, Yuko FUKUSHIMA, Hiroshi OHRUI, Hiroshi ...
1986 年 50 巻 1 号 p.
191-193
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Haruhito TSUGE, Toshikatsu ODA, Hideo MIYATA
1986 年 50 巻 1 号 p.
195-197
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Jeng-De Su, Toshihiko OSAWA, Mitsuo NAMIKI
1986 年 50 巻 1 号 p.
199-203
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Yasushi UDA, Yasuhiko MAEDA
1986 年 50 巻 1 号 p.
205-208
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Nobuhiko KOSUGI, Tomoyuki OKAMOTO, Hideshi YANASE, Kenzo TONOMURA
1986 年 50 巻 1 号 p.
209-212
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Katsuzumi OKUMURA, Koji IKURA, Hiroshi SAKURAI, Ryuzo SASAKI, Hideo CH ...
1986 年 50 巻 1 号 p.
213-215
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Mutsuo KANNO, Kyoko TORIYAMA
1986 年 50 巻 1 号 p.
217-218
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
-
Hiroyuki HIRANO, Masakazu IGUCHI, Takao NISHIMURA, Tatsuichi IWAMURA
1986 年 50 巻 1 号 p.
219-221
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
-
Tetsuo TOYOMASU, Takuo MATSUMOTO, Kan-ichi MORI
1986 年 50 巻 1 号 p.
223-225
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Takumi YOSHIZAWA, L.-M. COTE, S. P. SWANSON, W. B. BUCK
1986 年 50 巻 1 号 p.
227-229
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Takemasa KOJIMA, Kengo TABATA, Wataru ITOH, Toshio YANAKI
1986 年 50 巻 1 号 p.
231-232
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
-
Yoshinao KOIDE, Akira NAKAMURA, Takeshi UOZUMI, Teruhiko BEPPU
1986 年 50 巻 1 号 p.
233-237
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
The gene for an endolytic cellulase of
Bacillus subtilis was cloned on pBD64 in
B. subtilis. The recombinant plasmid, pBC5, contained a 4.8-kb fragment derived from the
B. subtilis chromosome, and the transformant overproduced the extracellular enzyme. The inserted fragment was subcloned on pBR322 and the recombinant plasmid, pBRC5, was introduced into
Escherichia coli JM105. The
E. coli transformant produced the enzyme in both the periplasmic space of cells and the cultural broth.
抄録全体を表示
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Masashi AWATA, Mitsuo HAYASHI, Naoki MUTO, Hideo SAKAKIBARA
1986 年 50 巻 1 号 p.
239-241
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Miyuki KURODA, Daisuke YOSHIDA, Shigenobu MIZUSAKI
1986 年 50 巻 1 号 p.
243-245
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Yoshifumi SHINMEN, Sumio ASAMI, Teruo AMACHI, Sakayu SHIMIZU, Hideaki ...
1986 年 50 巻 1 号 p.
247-249
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Khalid K. SADOZAI, Tomoo NUKADA, Yukishige ITO, Akira KOBATA, Tomoya O ...
1986 年 50 巻 1 号 p.
251-253
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Toshiaki SHINOHARA, Yasuo SETO
1986 年 50 巻 1 号 p.
255-256
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Yasutaka TAHARA, Yoshihiro OGAWA, Toyohiko SAKAKIBARA, Yuzo YAMADA
1986 年 50 巻 1 号 p.
257-259
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー
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Tsutomu NAKAYAMA, Motohisa KANEKO, Masahiko KODAMA
1986 年 50 巻 1 号 p.
261-262
発行日: 1986年
公開日: 2006/04/05
ジャーナル
フリー