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Masamichi KOSEKI, Hironobu SEKI, Masayoshi KAZAMA, Naofumi KITABATAKE, ...
1991 年 55 巻 6 号 p.
1441-1448
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
We examined the effect of pectin and lard on the production of short-chain fatty acids in the cecum, on the the growth of colonic bacteria, and on the liver and plasma cholesterol levels in rats. Added lard (12% w/w) in the diet lowered the production of acetic, propionic and butyric acids in the cecum, and decreased the mass of colonic bacteria. The addition of pectin (3%, w/w) to the diet with lard did not reduce the effect of the lard. The cholesterol level in the liver was significantly increased by the addition of 12% (w/w) of lard to the diet. Feeding with pectin did not lower the cholesterol level in the liver, which was correlated negatively with the concentrations of acetic and propionic acids in the cecum. When cholesterol was added to this diet, the liver and plasma cholesterol levels were increased by additing 12% (w/w) of lard to the diet.
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Kazumi YAGASAKI, Kouichi SAITO, Michihito YAMAGUCHI, Ryuhei FUNABIKI
1991 年 55 巻 6 号 p.
1449-1453
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
A possibility of involvement of arachidonic acid metabolism in insulin-stimulated protein systhesis was examined in cultured L6 myocytes, myoblasts and myotubes. L6 myoblasts required ten times as high concentrations of insulin as did L6 myotubes to attain comparable stimulatory actions on protein synthesis to L6 myotubes. The insulin-induced increase in protein synthesis was interrupted by a phospholipase A
2 inhibitor, mepacrine, and a cyciooxygenase inhibitor, indomethacin, in L6 myoblasts and myotubes, while the increase was not diminished at all by a lipoxygenase ihhibitor, caffeic acid, in both muscle cells. These results strongly suggest that arachidonic acid metabolite(s) produced
via cyciooxygenase pathway may be involved in the signaling cascade leading from the binding of insulin and cellular receptor to protein synthesis in L6 myoblasts and myotubes.
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Kiyoshi EBIHARA, Masayasu TAKEUCHI
1991 年 55 巻 6 号 p.
1455-1458
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
The effect of particle size on the surface area, cation-exchange capacity (CEC) and zinc-binding capacity of refined corn hull (RCH) was studied. The surface area was determined by the EGME method, and the specific surface area did not change substantially with particle size. CEC also did not change with particle size, and about half of the cation exchangeable group was strongly acidic. Zinc binding by RCH could be described by the Lungmiur's equation. While the zinc-binding capacity also did not change with particle size, it was highly pH-dependent, being greater at pH 6 than at pH 3.
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Keizou YAMAMOTO, Ken-ichi KOMATSU
1991 年 55 巻 6 号 p.
1459-1466
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
A nitrilase was purified, apparently to homogeneity, from a cell extract of
Acinetobacter sp. AK 226, which converts racemic 2-(4'-isobutylphenyl)propionitrile (Ibu-CN) to
S-(+)-2-(4'-isobutylphenyl)propionic acid (
S-(+)-ibuprofen). The molecular weight of the native enzyme was estimated as 580, 000 upon gel filtration. The nitrilase hydrolyzed many kinds of nitrite compounds such as aliphatic, aromatic, and heterocyclic mononitriles or dinitriles. The amino-terminal amino acids were sequenced and found to be partly homologous to a nitrilase from
Klebsiella pneumoniae subsp.
ozanae. The purified enzyme had a pH optimum of 8.0 and a temperature optimum of 50°C. The enzyme was not affected by chelating reagents, carbonyl reagents, reductants, most metal ions, or thiol reagents except silver ion,
p-chloromercuribenzoate, and phenylmercuribenzoate. The reaction with racemic Ibu-CN resulted in the preferential production of
S-(+)-ibuprofen, demonstrating that the nitrilase is highly enantioselective to
S-(-)-Ibu-CN. In fact, the enzyme showed a 180-fold higher activity for racemic Ibu-CN than that for
R-(+)-Ibu-CN.
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Noboru TAKIZAWA, Hirohumi SHIRO, Takashi HATTA, Kazutaka NAGAO, Hohzoh ...
1991 年 55 巻 6 号 p.
1467-1473
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
The pullulanase gene,
pulA, and the genes for pullulanase secretion factors (called the pullulanase secretion genes) from
Klebsiella aerogenes were cloned into a cosmid vector, pHC79, in
Escherichia coli. Four of one thousand clones isolated secreted pullulanase into their culture broth when the cells were grown with pullulan or maltose. One of the four clones produced much pullulanase extracellularly in a short time, with a level of production eightfold that of the maximum production of the wild-type
K. aerogenes W70. Deletion analysis of the recombinant plasmid showed that the pullulanase secretion genes and
pulA were all in a 22-kb DNA segment derived from the chromosomal DNA of
K.aerogenes.
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Katsumi SHIBATA, Michiko ONODERA, Shigeo AIHARA
1991 年 55 巻 6 号 p.
1475-1481
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
A rapid and convenient measurement of tryptophan in whole blood, serum, liver, brain, urine, and alkaline hydrolysates of proteins and foodstuff was done by high-performance liquid chromatography. The sample preparation was simply homogenized or mixed in a 5% trichloroacetic acid solution and a sample of the supernatant was injected onto a column after filtration with a 0.45-μm filter. The method used a Chemcosorb 5-ODS-H column (particle size, 5 μm, 150 × 4.6 mm i.d.) eluted with 20 mM potassium dihydrogen phosphate (pH adjusted to 3.7 by the addition of phosphoric acid) containing 1 g/l of sodium heptane sulfonate and 3 mg/l EDT A•2Na-acetonitrile (93:7, v/v) at a flow rate of 1.5 ml/min. The tryptophan contents in whole blood, serum, liver, and brain were electrochemically estimated at + 1000 mV
vs. Ag/ AgCl, the detection limit being 0.2 pmol (40.84 pg) at a signal-to-noise ratio of 5: 1. The tryptophan contents in urine, proteins, and foodstuff were fluorometrically estimated with an excitation wavelength of 280 nm and with an emission wavelength of 340 nm, the detection limit being 20 pmol (4.08 ng) at a signal-to-noise ratio of 5:1. Tryptophan was eluted at about 10.5 min. The total analysis time was about 12 min.
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Katsumi SHIBATA, Miyuki SHIOTANI, Michiko ONODERA, Takeshi SUZUKI
1991 年 55 巻 6 号 p.
1483-1490
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
We have reported that the excretion ratio of nicotinamide metabolites, [
N1-methyl-2-pyridone5-carboxamide (2-Py) +
N1-methyl-4-pyridone-3-carboxamide (4-Py)]/
N1-methylnicotinamide (MNA), is used as an index of amino acid adequacy. In this experiment, effects of protein-free diet feeding or starving on this excretion ratio were investigated using rats. The experimental period was 10 days. The urinary excretion of MNA, which is a major metabolites of nicotinic acid and nicotinamide, greatly increased with days, however, the urinary excretion of the MNA metabolites, 2-Py and 4-Py, decreased when rats were fed with a protein-free diet for 10 days. Accordingly, the resulting excretion ratio of (2-Py + 4-Py)/MNA in rats fed with the protein-free diet greatly decreased compared with that in rats fed with the 20% casein diet (control) (control vs. protein-free diet groups was 21.0 ± 3.5
vs. 0.5 ± 0.1 in urine samples on day 9-10). The urinary excretion of MNA did not increase when rats were starved for 10 days compared with that of the control. However, (2-Py + 4-Py)/MNA decreased to 3.6 ± 0.5 in urine samples on day 9-10. This decrease in the excretion ratio was attributed to the decrease in the activity of 2-Py- and 4-Py-forming MNA oxidase. These results further strengthen our claim that (2-Py + 4-Py)/MNA can be used as an index of amino acid adequacy.
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Yutaka HOSHIKAWA, Masako KIJIMA, Minoru YOSHIDA, Teruhiko BEPPU
1991 年 55 巻 6 号 p.
1491-1495
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
The effects of trichostatin A (TSA), a potent and specific inhibitor of mammalian histone deacetylase, on mouse teratocarcinoma cell (F9) differentiation were investigated. TSA caused marked changes in the morphology of cells and stimulated the production of plasminogen activator within 24 hr depending on
de novo synthesis of RNA and protein. During the initial 6 hr of incubation, a significant accumulation of hyperacetylated histone species was observed. These results suggest that histone acetylation due to inhibition of the deacetylase is involved in the early stage of morphological and biological differentiation induced by TSA.
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Nobuhiro KASHIGE, Misao KOJIMA, Kenji WATANABE
1991 年 55 巻 6 号 p.
1497-1505
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
The generation of active oxygen molecules, O
2-, H
2O
2, and •OH, from the aqueous solution of aminosugars, such as D-glucosamine, was confirmed by their actual measurement. Both the C-2 amino and C-1 aldehyde groups in the aminosugar molecules were indispensable for the generation of active oxygen molecules. The introduction of a C-6 phosphate group to D-glucosamine or the simultaneous use of phosphate ion and D-glucosamine heightened the original activity of D-glucosamine to generate these oxygens, especially •OH. Cu
2+, which promoted the DNA-breaking activity of aminosugar most at 1 mM, also promoted the generation of •OH most greatly at the same concentration, but neither O
2- nor H
2O
2 was generated under the same conditions. Superoxide dismutase, catalase, and some radical scavengers inhibited the generation of these active oxygen molecules. Among the active oxygen molecules, only the amount of •OH generated was directly proportional to the DNA-breaking activity of the aminosugar.
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Naoko TERASAWA, Masatsune MURATA, Seiichi HOMMA
1991 年 55 巻 6 号 p.
1507-1514
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
Nondialyzable model melanoidin prepared from glucose-glycine was separated into 6 components by pH gradient elution on a copper chelated Sepharose 6B column. The separated components were measured for copper chelating activity, UV-VIS spectrophotometry, and electrofocusing. The UV-VIS spectra of these components were found to be almost the same, but the melanoidin components with stronger affinity to the Cu column were found to be smaller in
Kd.
Their electrofocusing profiles were similar, and the electrofocused bands were categorized into four major groups with pi 2.5-4.0. Major chelating activity of the melanoidin components was due to a band of pi 2.7 on the electrofocusing.
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Setsuo FURUYOSHI, Jun NISHIGOURI, Nariyoshi KAWABATA, Hidehiko TANAKA, ...
1991 年 55 巻 6 号 p.
1515-1519
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
The production of D-glycerate from L-tartrate was investigated with cells of
Pseudomonas sp. group Ve-2, which produce L-tartrate decarboxylase. The production with intact cells had several advantages over production with the cell extract; no addition of NAD
+ is required, the substrate inhibition for the reaction is diminished, and the reaction can be done at higher temperatures than the reaction with cell extract. The culture conditions and the reaction conditions were optimized. The maximal activity was obtained when the cells were cultured at 30°C for 24 hr in a medium containing 1.0% potassium L-tartrate, 0.05% NH
4NO
3, 0.2% yeast extract, 0.3% K
2HPO
4, 0.01% MgSO
4•H
2O, and trace elements (pH 7.5). The amount of D-glycerate produced reached 106 g/l at 45°C after incubation for 45 hr, with a molar yield of almost 100%.
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Akihiro SHINZAKI, Yasuo AIZONO, Hiroshi YAMAGATA, Teruo IWASAKI, Minor ...
1991 年 55 巻 6 号 p.
1521-1530
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
Serine proteinase inhibitors I, IIa, and IIb were purified from the tubers of five
Solanum species to homogeneity by polyacrylamide gel disc electrophoresis. The purified inhibitors were characterized in terms of chemical, physicochemical, and immunochemical properties. Some slight but clear interspecific differences were noticed among the respective types of inhibitor molecules. Based on the differences, the five
Solanum species were classified into two groups. The first group included three tetraploid species,
i.e.,
S. tuberosum ssp.
tuberosum (common potato),
S. tuberosum ssp.
andigena (an Andean cultivated species), and an artificial amphidiploid of
S. stenotomum ×
S. phureja. The second group consisted of
S. acaule (a wild tetraploid species), and
S. juzepczukii (a natural triploid hybrid between
S. acaule and
S. stenotomum). This classification is in good agreement with the phylogenetic relations so far known among the
Solanum species tested.
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Jin Ye WANG, Teruo MIYAZAWA, Kenshiro FUJIMOTO
1991 年 55 巻 6 号 p.
1531-1536
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
When stored at high relative water activity (
Aw 0.9), zein has a prominent antioxidant activity against the peroxidation of methyl linoleate, while its papain hydrolysate does not. Using a lipid-soluble radical initiator, AMVN [2, 2'-Azobis(2, 4-dimethyl-valeronitrile)], to alter the induction period of peroxidation of methyl linoleate, we found a turning point just at 24 hr where the rate of oxygen absorption declined. The formation of a solid, tightly aggregated outer shield was observed by scanning electron microscopy. A rather amount of embedded substrate oil (methyl linoleate-methyl stearate, 7:3) was obtained when aggregations formed, and there was little change in the level of embedded oil for at least 16 days. On the other hand, a hydrolysate of zein had scarcely any embedding effect. The ratio of methyl linoleate to methyl stearate showed that embedded oil (hexane unextractable) was more protective to oxidation than hexane-extractable oil. These findings indicate that the substrate oil must have been embedded in the shield formed by zein protein at
Aw 0.9 to avoid outer oxygen attack.
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Joji OKUMURA
1991 年 55 巻 6 号 p.
1537-1545
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
Volatile compounds formed from model systems of equimolecular amounts of L-cysteine and propanal reacted at 110°C for 3hr in triglyceride, deionized and distilled water, or their mixtures were investigated. The volatiles were isolated by ether extraction of vacuum steam-distillates of reaction mixtures and analyzed by gas chromatography and coupled gas chromatography-mass spectrometry. Qualitative and quantitative differences in volatiles formed in the systems were observed. The amounts of total volatiles were highly varied according to the composition of the reaction medium. 2-Ethylthiazolidine was the most predominant in the triglyceride system. 2-Methyl-2-pentenal was a secondary dominating product in the triglyceride system and the most predominant in the triglyceride-water and water systems. The amounts of other volatile compounds including a novel compound, 2-ethylthiazolidino[3, 4-
b]thiazolidine (I), were also changed according to the systems. The highest production of I was observed in the triglyceride-water (75:25) system.
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Junji WATARI, Yoshihiro TAKATA, Masahiro OGAWA, Jim MURAKAMI, Shohei K ...
1991 年 55 巻 6 号 p.
1547-1552
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
We have cloned a DNA fragment bearing the putative
FLO1 gene that controls flocculence in
Saccharomyces cerevisiae and showed that the cloned gene is on chromosome I. The flocculation property conferred by the cloned gene in a YCp-type plasmid (single-copy plasmid) was diminished in
MATa/
MATα. cells, which suggests that the expression of the putative
FLO1 gene may be repressed by the
MAT information. Introduction of the DNA fragment in a YRp-type plasmid (multi-copy plasmid) into several non-flocculent industrial yeast strains, including bottom- and top-fermenting brewing yeasts, and those for making whisky, wine, sake, and shochu could convert them to flocculent ones.
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Bunsei KAWAKAMI, Hilzheber CHRISTOPHE, Masataka NAGATOMO, Masanori OKA
1991 年 55 巻 6 号 p.
1553-1559
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
The genes of the
AccI restriction-modification system specific for GT(A/C) (G/T)AC were cloned from the chromosomal DNA of
Acinetobacter calcoaceticus, and their nucleotides sequenced. The restriction and modification genes coded for polypeptides with calculated molecular weights of 42, 494 and 63, 078, respectively. Both the enzymes were coded by the same DNA strand and the restriction gene was upstream of the methylase gene, separated by 2 bp. The restriction gene was significantly expressed in
E. coli cells, so that the
AccI restriction endonuclease could be purified to homogeneity. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration indicated that the catalytically active form of the endonuclease was tetrameric. Sequence comparison with related enzymes indicated that
AccI methylase contained a segment of tetra-amino acids, NPPY, characteristic of N6-adenine methylases. In addition, some homologous regions were found in the sequence of
HincII methylase specific for GT(C/T) (A/G)AC.
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Nobutaka SUZUKI, Taro ITAGAKI, Akio GOTO, Binkoh YODA, Tateo NOMOTO, I ...
1991 年 55 巻 6 号 p.
1561-1564
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
9-Acridone-2-suIfonic acid was shown to react specifically with superoxide to give chemiluminescent light emission in an aqueous media as well as in dimethyl sulfoxide (DMSO). Thus, it supplements the non-specificity of
Cypridina luciferin analogues to singlet molecular oxygen and the superoxide anion radical.
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Jean-Marc BRILLOUET, Pascale WILLIAMS, Michel MOUTOUNET
1991 年 55 巻 6 号 p.
1565-1571
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
An endo-β-(1→6)-D-galactanase has been purified 26-fold (14% activity yield) from a crude enzyme preparation from
Aspevgillus niger. The enzyme was homogeneous on PAGE and SDS-PAGE, with an apparent molecular mass of 60 kDa. It has maximum activity at pH 3.5 on an enzymatically desarabinosylated grape 3, 6-galactan-protein (Gp), being unreactive towards the native grape AGp. The enzyme liberated β-(1→6)-D-galactobiose and galactose in a final 1:0.26 molar ratio, β-(1→3)-D-galactosidic bonds being resistant to the action of enzyme.
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Nobuko MINAGAWA, Shigeru SAKAJO, Akio YOSHIMOTO
1991 年 55 巻 6 号 p.
1573-1578
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
Cyanide-resistant respiration was induced in
Hansenula anomala in the presence of various sulfur compounds, having no inhibitory effect on the normal cytochrome pathway. This induction was observed in the presence of glucose. The mitochondrial 36kDa protein responsible for cyanide-resistant respiration was also detected in the cells where cyanide-resistant respiration was induced by oxidized glutathione, indicating that there might be a common mechanism to initiate the biosynthesis of the protein. All the inducing conditions are deduced to increase the generation of superoxide anion, and some radical scavengers inhibited the induction, suggesting the possible involvement of certain active oxygen species in the initial induction process.
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Hideki OHBA, Nobuyuki YAMASAKI, Gunki FUNATSU
1991 年 55 巻 6 号 p.
1579-1585
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
Chemical modification of tryptophan residues in abrin-a with
N-bromosuccinimide (NBS) was studied with regard to saccharide-binding. The number of tryptophan residues available for NBS oxidation increased with lowering pH, and 11 out of the 13 tryptophan residues in abrin-a were eventually modified with NBS at pH 4.0, while 6 tryptophan residues were modified at pH 6.0 in the absence of specific saccharides. Modification of tryptophan residues at pH 6.0 greatly decreased the saccharide-binding ability of abrin-a, and only 2% of the hemagglutinating activity was retained after modification of 3 residues/mol. When the modification was done in the presence of lactose or galactose, 1 out of 3 residues/mol remained unmodified with a retention of a fairly high hemagglutinating activity. However, GalNAc did not show such a protective effect. NBS-oxidation led to a great loss of the fluorescence of abrin-a, and after modification of 3 tryptophan residues/mol, the fluorescence intensity at 345 nm was only 38% of that of the unmodified abrin-a. The binding of lactose to abrin-a altered the environment of the tryptophan residue at the saccharide-binding site of abrin-a, leading to a blue shift of the fluorescence spectrum. The ability to generate such fluorescence spectroscopic changes induced by lactose-binding was retained in the derivative in which 2 tryptophan residues/mol were oxidized in the presence of lactose, but not in the derivative in which 3 tryptophan residues/mol were oxidized in the absence of lactose. Importance of the tryptophan residue(s) in the saccharide-binding of abrin-a is suggested.
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Hiroyuki KATAOKA, Yukizo UENO, Masami MAKITA
1991 年 55 巻 6 号 p.
1587-1592
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
A sensitive and selective method was developed for the analysis of
O-phosphoserine (P-Ser),
O-phosphothreonine (P-Thr) and
O-phosphotyrosine (P-Tyr) in proteins by gas chromatography (GC). After partial acid and base hydrolysis of the protein samples, the liberated
O-phosphoamino acids were converted into their
N-isobutoxycarbonyl trimethyl ester derivatives and measured by GC with flame photometric detection (FPD-GC), using a DB-1701 capillary column. The calibration lines were linear in the range of 0.1-5nmol for P-Ser and P-Thr, and in the range of 0.05-1 nmol for P-Tyr, and the detection limits for P-Ser, P-Thr and P-Tyr were about 0.26, 0.18 and 0.30 pmol as injection amounts, respectively. The best hydrolysis conditions for the proteins proved to be 6M HCl at 110°C for 2hr to analyze P-Ser and P-Thr, and 5M KOH at 130°C for 1hr to analyze P-Tyr.
O-Phosphoamino acids in the protein hydrolyzates could be selectively determined by the FPD-GC method without any influence from other amino acids. This method was applied to the analysis of
O-phosphoamino acids in several commercial proteins.
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Keiko HATAE, Shugo WATABE, Yasuo OKAJIMA, Masataka SHIRAI, Atsuko SHIM ...
1991 年 55 巻 6 号 p.
1593-1600
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
Live carp was killed instantly, and the sliced muscle was subjected to four different "arai" preparations, that is, washing at 18°C and 49°C in water, and thawing at 4°C and 20°C after freezing at - 20°C. Transmission electron microscopy revealed that the structural integrity of myofibrils decreased in the 18°C and 49°C washed samples and in the 20°C thawed sample. Curved myofibrils appeared in the 18°C washed and 20°C thawed samples, while black and white stripes appeared with the 49°C washed sample, suggesting a drastic change in myofibrillar structure during washing. This degree of change in myofibrillar ultrastructure was related to the level of toughening in texture as well as to ATP depletion.
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Hiroki TOMIOKA, Toshihiko YANO, Yoji TAKADA, Hisami TAKEDA, Naonori HI ...
1991 年 55 巻 6 号 p.
1601-1606
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
Structure-activity relationships of novel 1-alkoxymethyl-2, 4, 5-trihaloimidazole compounds against a wild colony of German cockroaches resistant to pyrethroids (O-colony) are reported. Among them, 1-methoxymethyl-2-bromo-4, 5-dichloroimidazole and 1-isopropoxymethyl-2-bromo-4, 5-dichloroimidazole were very effective by a filter paper contact method. The variation in insecticidal activity is shown to be related to the length and branching of the alkyl chain of the 1-substituents. Above all, 1-(4-bromobutoxymethyl)-2-bromo-4, 5-dichloroimidazole and l-(4-bromo/chlorobutoxymethyl)-2, 4, 5-trichloroimidazole exhibited low acute oral toxicity to mice and good activity by a heating fumigant test, representing one of the practical applications. The trihaloimidazole derivatives had almost two-fold higher activity against O-colony insects than against the susceptible strain.
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Shingo KANOH, Haruhiko MASAKI, Shunsuke YAJIMA, Takahisa OHTA, Takeshi ...
1991 年 55 巻 6 号 p.
1607-1614
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
Colicin E2 is encoded by the SOS-inducible colicin operon of plasmid ColE2-P9. Highly induced colicin E2 is excreted from the
E. coli host cell, accompanied by quasi-lysis and cell death. These three events are all directed by the colicin lysis protein that comprises the signal peptide and a small mature lipoprotein. We constructed a mutant lysis gene encoding its complete signal peptide with addition of two external amino acids at the C-terminus. By using this mutant peptide as a reference, the wild type lysis signal peptide was identified and shown to remain quite stable after the cleavage. This mutant peptide also mimics the cleaved-off signal peptide avoiding the influence of the mature lysis lipoprotein, and suggested that cell death by the lysis protein, but neither colicin excretion nor quasi-lysis, is decided by the nature of its signal region.
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Junya YAMAMOTO, Mikio SHIMIZU, Kunio YAMANE
1991 年 55 巻 6 号 p.
1615-1626
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
A 3.8-kb
EcoRI-fragment containing the
lysA gene [diaminopimelate (DAP) decarboxylase] of
Bacillus subtilis has been cloned into
B. subtilis phage ø105 and its nucleotides sequenced. The nucleotide sequence of a 3, 762 bp stretch contained three open reading frames (ORF1, ORF2, and ORF3) in one orientation and another open reading frame (ORF4) in the opposite orientation. ORF2 coded for the
lysA gene based on the complementation of a
B. subtilis lys auxotroph and on the fact that the predicted amino acid sequence (440 amino acids with a molecular weight of 48, 876) of ORF2 shared a 29.7%, 38.3%, and 32.9% identity with the sequences of
Escherichia coli,
Corynebacterium glutamicum and
Pseudomonas aeruginosa lysA genes, respectively. ORF1, ORF3, and ORF4 did not correspond to
E. coli lysR. Based on the comparison of the
B. subtilis lysA sequence with a sequence of the DAP-decarboxylase gene cloned into pUB110 (Yamamoto
et al., Nucleic Acids Res., 17, 10105 (1989)), it was found that the
lys gene in the plasmid was fused with the
dnaN gene in its COOH-terminal region.
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Masatoshi YAMAMOTO, Toshio MASUI, Kiyoshi SUOIYAMA, Masami YOKOTA, Kaz ...
1991 年 55 巻 6 号 p.
1627-1629
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
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Kazuhisa TOYODA, Kuniyo INOUYE
1991 年 55 巻 6 号 p.
1631-1633
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
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Seiji SUGIMOTO, Yoshiharu YOKOO, Yasuo INUI, Tetsuya HIRANO
1991 年 55 巻 6 号 p.
1635-1637
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Eric D. BROWN, Rickey Y. YADA
1991 年 55 巻 6 号 p.
1639-1641
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Claudio FUGANTI, Giuseppe PEDROCCHI-FANTONI, Stefano SERVI
1991 年 55 巻 6 号 p.
1643-1644
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Hachiro OZAKI, Reiko MAKIHARA, Takao KIDA, Shigeru NAKAMORI
1991 年 55 巻 6 号 p.
1645-1646
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Masaru KITAGAWA, Kimikazu IWAMI, Fumio IBUKI
1991 年 55 巻 6 号 p.
1647-1648
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Masaru KITAGAWA, Kimikazu IWAMI, Fumio IBUKI
1991 年 55 巻 6 号 p.
1649-1650
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Naotoshi MATSUDOMI, Michiya TAKASAKI, Kunihiko KOBAYASHI
1991 年 55 巻 6 号 p.
1651-1653
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Yoshiko KUROBAYASHI, Hidemasa SAKAKIBARA, Tetsuya YANAI, Izumi YAJIMA, ...
1991 年 55 巻 6 号 p.
1655-1657
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Hiroshi KAMITANI, Nobuyoshi ESAKI, Hidehiko TANAKA, Kenji SODA
1991 年 55 巻 6 号 p.
1659-1660
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Tetsuya OGUMA, Mamoru KIKUCHI, Kiyoshi MIZUSAWA
1991 年 55 巻 6 号 p.
1661-1662
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Kunihiko GEKKO, Yuki KUWANA, Takuji SASAKI, Shio MAKING
1991 年 55 巻 6 号 p.
1663-1664
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Atsuyoshi NISHINA
1991 年 55 巻 6 号 p.
1665-1667
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Yoshinobu KATOH, Yoshio SANO
1991 年 55 巻 6 号 p.
1669-1670
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Shonosuke SAGISAKA, Haruyuki KURODA
1991 年 55 巻 6 号 p.
1671-1673
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Yoshiki YAMASAKI, Haruyoshi KONNO
1991 年 55 巻 6 号 p.
1675-1676
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Isao SODA, Tadao HASEGAWA, Takao SUZUKI, Nagao OGURA
1991 年 55 巻 6 号 p.
1677-1678
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Hirofumi SHINOYAMA, Kenro TAKEI, Akikazu ANDO, Takaaki FUJII, Makoto S ...
1991 年 55 巻 6 号 p.
1679-1681
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Shigehiro HIRANO, Hiroaki KANEKO, Motoko KITAGAWA
1991 年 55 巻 6 号 p.
1683-1684
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Takashi EBATA, Hiroshi KAWAKAMI, Koshi KOSEKI, Hajime MATSUSHITA
1991 年 55 巻 6 号 p.
1685-1686
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Hiroshi KAWAKAMI, Takashi EBATA, Hajime MATSUSHITA
1991 年 55 巻 6 号 p.
1687-1688
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Sanetaka SHIRAHATA, Hirofumi TACHIBANA, Tetsuya MORI, Kiyohiko SEKI, K ...
1991 年 55 巻 6 号 p.
1689-1691
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー
-
Sawao MURAO, Homare ITOH, Shigeki FUKUYASU, Kazuo HIRAYAMA, Takashi SH ...
1991 年 55 巻 6 号 p.
1693-1694
発行日: 1991年
公開日: 2006/04/05
ジャーナル
フリー