TIA-1 and granzyme B are cytotoxic granule proteins in T cells and natural killer cells. They have been found to be involved in the induction of apoptosis and are manifested in some type of cutaneous T cell lymphoma. We examined immunohistopathological studies of component subsets of the lymphocytic infiltrates in inflammatory skin disorders, mainly histopathologically characterized lichenoid tissue reaction. A total of 73 skin biopsies were obtained from 13 cases of lichen planus, 5 cases of lichenoid drug eruptions, 14 cases of discoid lupus erythematodes, 4 cases of lupus erythematodes profundus, 12 cases of systemic lupus erythematosus, 11 cases of toxicoderma, 5 cases of psoriasis vulgaris, 7 cases of chronic eczema, and 2 cases of lymphadenitis benigna cutis. The formalin-fixed paraffin-embedded tissues were stained using the labeled streptavidinbiotinylated antibodies method with antisera to UCHL-1, CD 4, CD 8, CD 79 a, CD 30, CD 56, CD 68, S-100, HLA-DR, TIA-1, and Granzyme B. Results : Most cells in the dermal infiltrates stained with UCHL-1 (T cells) antibodies and with CD 8 (suppressor cytotoxic T cell). These cells particularly expressed TIA-1 in lichen planus and lichenoid drug eruption. A double-staining method using antibodies for CD 8 and TIA-1 was performed ; concomitant TIA-1-positive and CD 8-positive cells were found in lesions with severe basal damage.
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