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Volume 27 (1988)
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- Issue 5 Pages 659-
- Issue 4 Pages 449-
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- Issue 2 Pages 161-
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Volume 27, Issue 4
Displaying 1-22 of 22 articles from this issue
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Eiji OHNO, Hiroyuki KURAMOTO1988 Volume 27 Issue 4 Pages 449-458
Published: July 22, 1988
Released on J-STAGE: December 05, 2011
JOURNAL FREE ACCESSIn order to establish the cytological criteria in diagnosing the histological grading of endometrial adenocarcinoma, seventy-one cellular samples (G1 16, G2 35, G3 7 and atypical endometrial hyperplasia 13) were examined retrospectively. All samples were obtained from endometrial cavity by endocyte technique and stained with Papanicolaou method. Positivity in alkaline phosphatase (ALP) staining and nuclear diameters were also evaluated in each group of the differentiation.
1. Cytological findings to differentiate G 1 carcinoma from atypical endometrial hyperplasia (AEH) were realized as follows:(1) cellular cluster with irregular margin just like twigs growing from a tree, (2) cluster with nuclei protruding from the margin, (3) irregularly shaped nuclei, (4) thickening of the nuclear border, (5) unevenly distributed chromatin, (6) multiple nucleoli, and (7) tumor diathesis. Each finding was remarkably predominant in G 1 carcinoma.
2. Differences among G 1, G 2 and G 3 carcinomas were follows;(1) clusterizing rate of malignant cells, (2) anisokaryosis, (3) increased nuclear size, (4) coarse chromatin, (5) unevenly distributed chromatin, (6) whitish euchromatin, (7) large nucleoli, (8) histiocytes, (9) tumor diathesis, and (10) coexisting normal endometrial cells. Items (2) to (9) were remarkable in the more undifferentiated group, whereas items and (10) were conspicuous in the more differentiated group. Anisokariotic tendency in the more undifferentiated group was also confirmed by nuclear measurement.
3. ALP staining revealed positive in 59.0% among whole cases examined, and well differentiated carcinoma and AEH groups showed positive in 80%, whereas poorly differentiated one in 16.7%. Diagnosis of the histological grading of endometrial carcinoma by conventional cytology be more accurate when combined with the cytochemical approach.View full abstractDownload PDF (11595K) -
Kazushi SHIGEMASA, Yoshihide TANIOKA, Hiroshi MATSUDA, Nobutaka NAGAI, ...1988 Volume 27 Issue 4 Pages 459-467
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSImmunocytochemical detection of CA 125 and epithelial membrane antigen (EMA) was performed in cancer cells, mesothelial cells and histiocytes in ascitic fluid specimens obtained from patients with ovarian serous cystadenocarcinomas using the avidin-biotin-peroxidase technique. In addition, immunohistochemical localization of CA 125 and EMA in tissue specimens from the same patients were examined. CA 125 and EMA in mesothelial cells of non-cancerous ascitic fluid were also examined.
The results were as follows;
(1) In the tissue specimens, CA 125 was detected in 9 of 10 cases and EMA was detected in all 10 cases. Both CA 125 and EMA appeared mainly on the luminal surface of the cancer cells. In addition, positive stains of EMA were observed even in the cytoplasm in some cases.
(2) In cancer cells of the ascitic fluid, both CA 125 and EMA were positive in all cytologic specimens. Immunocytochemical double stains with CA 125 and EMA revealed that more cancer cells demonstrated a positive reaction for CA 125 than for EMA in all cases.
(3) Some of the mesothelial cells in both cancerous and non-cancerous ascitic fluid showed a positive reaction for CA 125, and the reactive mesothelial cells showed staining similar to that of cancer cells. EMA was only weakly expressed on a few reactive mesothelial cells.
(4) In inflammatory cells like histiocytes that were present in the ascitic fluid, both CA 125 and EMA were negative in all cytologic specimens.
The above findings suggest that immunocytochemical stainings of CA 125 and EMA in ascitic cytology is useful in detecting cancer cells and differentiating them from reactive mesothelial cells.View full abstractDownload PDF (6844K) -
Satoru MUNAKATA1988 Volume 27 Issue 4 Pages 468-476
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSFlow cytometric measurement requires single cell suspension. We used human squamous epithelium of the uterine cervix, endocervical epithelium and endometrium of 51 uteri to evaluate methods for disaggregating tissues to obtain single cell suspensions. For tissue dispersion both chemical (trypsin, collagenase, EDTA and dispase) and mechanical methods (ultra-disperser, pipetting and syringing) were used. The following methods of tissue dispersion are recommended:(1) cervical squamous epithelium, 2, 000 U/ml dispase for 60 min and 25, 000 r. p. m. ultra-disperser for 30 sec;(2) endocervical epithelium, 1, 000-2, 000 U/ml dispase for 60 min and 25, 000 r. p. m. ultra-disperser for 10 sec;(3) endometrial epithelium, 1, 000-2, 000 U/ml dispase for 60 min and 21-gauge syringing 10 times.
After tissue dispersion, cell suspensions were fixed by 50% ethanol and kept at 4°C until flow system analysis. Before analysis the cell suspension was stained by propidium iodide (PI) and fluorescein isothiocyanate (FITC). All epithelial cells showed the peculiar FCM two-parameter histogram pattern:(1) Squamous cells showed a euploid pattern, and protein fluorescence showed much variety.(2) Endocervical cells showed a diploid pattern, and protein fluorescence was low.(3) Endometrial cells showed a diploid pattern, and protein fluorescence was lower than that of endocervical cells.View full abstractDownload PDF (7101K) -
Immunohistochemical double staining with anti-BrdU monoclonal antibodyMorimasa MATSUTA, Toshihiko IZUTSU, Iwao NISHIYA, Mikiko ASANUMA, Shun ...1988 Volume 27 Issue 4 Pages 477-483
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA patient of ovarian cancer with hyperamylasemia was treated by chemotherapy and surgical specimens were obtained after the labelling in vivo with bromodeoxyuridine (BrdU).
Amylase was detected in the cytoplasm and BrdU was localized in the nuclei of neoplastic cells by immunoperoxidase method (IPX), and amylase positive cells showed no positive image of BrdU.
Labelling index, determined by IPX with anti-BrdU monoclonal antibody, revealed 11.6% and less than 1% before and after chemotherapy, respectively.
Since amylase was considered to express the characteristics of cytoplasm and BrdU showed the function of nuclei, it was seemed amylase and BrdU would be regard as independent parameters in the ovarian cancer. Immunohistochemical double staining, adopted on stamp smear, clarified the relation between the localization of amylase and that of BrdU at a time. Immunohistochemical double staining was regarded as a useful technique on histological and cytological reseaches.View full abstractDownload PDF (11409K) -
Changes in oral mucosa depending on area, sex, age, complete denture, and pregnancyRyukei MIYASHITA, Shigeki YABE, Hideo OSAWA, Takashi JOSHITA1988 Volume 27 Issue 4 Pages 484-497
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThe purpose of this study was to obtain a clearer understanding, from a cytological point of view, of changes in oral mucosa according to area, sex, age, pregnancy, and especially denture use. One hundred thirty-five healthy subjects, including children, adults, and the aged, were investigated. The aged subjects were classified as denture wearers and nonwearers. The following six different areas of oral mucosa were examined from the viewpoint of exfoliative cytology and, to some extent, histology: hard palate, soft palate, cheek, maxillary anterior gingiva, mandibular anterior gingiva, and lower labium.
FINDINGS:
1. A high rate of keratinization was found in areas of hard palate and maxillary and mandibular gingiva regardless of sex or age, while the rate was lower in areas of the cheek, soft palate, and lower labium.
2. A difference in the rate of keratinization due to sex was recognized among adults and aged denture wearers and non-wearers. Similar results were not found in children. Females showed a lower tendency toward keratinization in the gingiva than males. A similar tendency was found in the hard palate of denture wearers. These findings seem to indicate that sex hormones play a role in changes in keratinization in the oral mucosa.
3. Changes in keratinization with age were identical in both males and females. The rate of keratinization in gingiva increased from childhood to adulthood, then decreased with advancing age.
4. A marked decrease in keratinization was observed in the hard palate of both male and female denture wearers. Similar results with a less marked decrease were also obtained for the gingiva.
5. A noticeable decrease in keratinization, mainly in the area of the gingiva, was recognized among pregnant as compared with non-pregnant women.
6. Exfoliative cytological findings were generally consistent with histological findings.View full abstractDownload PDF (15495K) -
Kosaku OMATA, Noboru TANAKA1988 Volume 27 Issue 4 Pages 498-506
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSOne hundred and four asymptomatic papillary carcinomas of the thyroid were incidentally detected in a mass screening using ultrasonic echography. The patients were operated upon and 53 (51%) carcinomas were 1 cm or less in diameter. The nuclear DNA content of 46 specimens was examined by echo-guided aspiration cytology and microfluometry.
These papillary carcinomas were histologically classified into four types according to the degree of fibrous encapsulation and stromal fibrosis; non-encapsulated and slightly sclerosing carcinoma (Type A), non-encapsulated and markedly sclerosing carcinoma (Type B), encapsulated and sclerosing carcinoma (Type C) and slightly encapsulated and nonsclerosing carcinoma (Type D). The mode of distribution of DNA was classified into three groups: Group II a (diploid, less than 10% of over 3C cell), Group II b (diploid, more than 10% of over 3C cell) and Group III (diploid + aneuploid). Most papillary carcinomas with clinical manifestations were Type C or D histologically and showed a Group-III DNA distribution pattern.View full abstractDownload PDF (7390K) -
Hitoshi ITO, Sayeste Demirezen, Reiko SHINODA, Yuko AKATSUKA, Kenichi ...1988 Volume 27 Issue 4 Pages 507-511
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSImmunocytochemical localization of keratin was studied in order to evaluate the difference between cancer cells and non-malignant cells of the breast on cytological smears and tissue sections using indirect immunoperoxidase technique.
In cytological specimens as well as histological specimens adenocarcinoma cells showed distribution of keratin along cell membrane at periphery of the cell, but benign cells had strong lump-like staining for keratin at the luminal side in the cytoplasm. These result suggested that distribution of keratin might be changed by malignant transformation and that the difference of distribution might be helpful in differential diagnosis of aspiration biopsy specimens of the breast.View full abstractDownload PDF (7497K) -
Kinya SAWADA, Kimito MATSUMURA, Hideo IKEDA, Tetsusi HIRATA, Hiroyuki ...1988 Volume 27 Issue 4 Pages 512-517
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSCytological diagnosis of so-called sclerosing hemoangioma of the lung is not well established. The purpose of present study was to correlate the cytomorphological and pathohistological findings of imprinted smears from 7 resected cases.
The cytological characteristics of sclerosing hemoangioma can be summarized as follows:
1. Papillary arrangements showed papillary clumping like adenocarcinoma cells.
2. Dispersed flatty cells were cuboidal and showed a mild degree of atypism.
3. Numerous cell types were differentiated in smears, e. g., papillary inflammatory cells and so on.View full abstractDownload PDF (6325K) -
Junko FUKUTOMI, Takako IWATA, Mutsuo TAKAHASHI, Masaru YAMASHITA, Yosh ...1988 Volume 27 Issue 4 Pages 518-530
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSTissue cultures from body fluids of three cases were studied to clarify morphological characteristics of mesothelial cells in an in vitro system. These materials were obtained from ascites of a patient that had hepatoma with liver cirrhosis, and pleural fluids of a patient with metastatic gastric cancer and one with malignant mesothelioma. Immunocytochemical, enzyme histochemical and scanning electron microscopical studies of the cultured cells revealed the following results:
1) Proliferated cells in vitro were divided into three types; epithelial-like (E-type), fibroblast-like (F-type) and giant-cell types (G-type).
2) With scanning electron microscopy, many microvilli and a few pinocytic vesicles were observed on the surface of cultured mesothelial cells. These findings were in contrast to free-floating mesothelial cells whose surfaces were mainly covered by vesicles or blebs. From the present results, it is suggested that the cultured mesothelial cells, which settled to the glass surface, regained microvilli and pinocytic vesicles like mesothelial cells lining the serous body cavities.
3) The cells from a patient with malignant mesothelioma proliferated easily in vitro and piled up. They exhibited more prominent morphological atypism than normal mesothelial cells in tissue culture.View full abstractDownload PDF (25397K) -
Shinobu NAKAMURA, Yasushi TAKEDA, Kazumi KOBAYASHI, Takashi YOSHIDA, S ...1988 Volume 27 Issue 4 Pages 531-535
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSTo determine the labeling index (LI) of bromodeoxyuridine (BrdU) on human leukemic cells in vivo, six patients with acute leukemia were administrated 200 mg/m2 of BrdU intravenously prior to chemotherapy. After 30 minutes, bone marrow was aspirated and air-dried smears were made. Each smear was stained immunohistochemically using a monoclonal antibody to BrdU and the LI of leukemic cells was estimated.
Results were as follows:
1. LIs of leukemic cells in bone marrow were 3.0% to 7.5%, almost identical to those calculated using tritiated thymidine or a drip infusion of BrdU in vivo. This seems to indicate rapid incorporation of BrdU into S-phase cells, which can be used to analyze cell-cycle kinetics in acute leukemia.
2. BrdU-labeled cells were easily differentiated from unlabeled ones using air-dried smears stained by the indirect immunoenzyme method.
3. In smears counterstained by Giemsa stain, it was possible to identify leukemic cells. However, Giemsa stain did not permit a closer examination of cells.
4. To obtain good specimens, it was essential to dry smears for at least 2 hours.View full abstractDownload PDF (3823K) -
Fujiko ITO, Atuo MORI, Kiichi OGAWA, Motoo HANANOUCHI, Haruo HAYASHI1988 Volume 27 Issue 4 Pages 536-540
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of adenocarcinoma in situ of the uterine cervix in a 32-year-old woman is reported. We found atypical glandular cells on vaginal and endocervical smears which suggested well-differentiated adenocarcinoma. Definitive surgery with modified radical hysterectomy was performed. The pathological diagnosis was adenocarcinoma in situ of the uterine cervix.
The cervical smear showed clusters of tall columnar atypical cells arranged in sheets or palisades. Their enlarged nuclei were oval in shape and slightly hyperchromatic with fi nely to moderately granular chromatin patterns, and displayed inconspicuous nucleoli.View full abstractDownload PDF (4042K) -
Taku KATOH, Hisao TAKAHASHI, Takashi KIYOKAWA, Bin TAKEDA, Fumio HORIU ...1988 Volume 27 Issue 4 Pages 541-548
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSProliferating Brenner tumor of ovary belongs to low potential malignancy and is clinically as rare as malignant Brenner tumor.
Cytological features of this tumor are not yet reported in the previous report. We reported a case of Proliferating Brenner tumor in right ovary with cytological and ultrastructural findings. Epithelial cells appeared in the imprints smear, are atypical transitional cells, columnar cells and squamous metaplastic cells. Immunocytologic staining indicated CEA positive reaction in atypical transitional cells and squamous metaplastic cells, EMA positive in these three kinds of cells.
Macroscopically it is 14×13×12cm sized cystic tumor with intracystic papillary growth and histologically proliferating tumor without stromal invasion and metastasis to other organs.
Ultramicroscopic studies proved consistent figures with above-mentioned three types of cells.
No hormonal influence was found in vaginal squamous cell maturation and in histological endometrial gland in this tumor.View full abstractDownload PDF (17651K) -
Akira TANIMURA, Yasuaki FUJIYOSHI, Kozue MASAIKE, Toyohide YANAI, Tsun ...1988 Volume 27 Issue 4 Pages 549-552
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe presented a case of chordoma with pseudocilia in the brain of 44 year-old male patient.
Cytologically tumor cells of chordoma had long pseudocilia and tumor cells in frozen sections also showed columnar cells in epithelial-like arrangement with pseudocilia. Paraffinembedded sections after formalin fixation showed chordoma with abundant PAS-alcian blue positive rich stroma, but with no pseudocilia as seen in cytology.
Using an immunoperoxidase technique, these tumor cells were positive for EMA (epithelial membrane antigen), keratin and S-100 protein.View full abstractDownload PDF (3844K) -
Kenji TANAKA, Hikotaro KOMATSU, Takashi ISHIHARA, Shokichi TAJIMA1988 Volume 27 Issue 4 Pages 553-557
Published: July 22, 1988
Released on J-STAGE: December 05, 2011
JOURNAL FREE ACCESSA case of chordoma arising from the posterior mediastinum was reported.
A 33 year-old man came to our hospital for evaluation of an abnormality on chest X-ray film.
A thoracic CT scan demonstrated a mass in the superior posterior mediastinum with vertebral involvement.
Under a preoperative diagnosis of malignant mediastinal tumor, the operation was carried out and the majority of tumor was removed.
The cytological findings of imprint smear from obtained tumor revealed many atypical cells were arranged in cords and sheets in a mucinous background and many clear vacuolus in cytoplasm which were so called physaliphorous cells.
Histologically, the cytoplasm of tumor cell showed stronglypositive reaction for PAS staining and in contrast the intercellular space was positive for alcian blue staining.
Ultrastructually, the tumor cells contained many vacuoles, microfilaments, microvilli, desmosomes, rough ER and glycogen granules in cytoplasm.
The histogenesis of the cytoplasmic vacuoles was briefly discussed.View full abstractDownload PDF (10373K) -
Seiichi HIROTA, Yoshiki NAKAGAWA1988 Volume 27 Issue 4 Pages 558-563
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of carotid body paraganglioma was reported. 60 year-old woman had noticed a right neck tumor for two years, and aspiration cytology for diagnosis was carried out. Cytological specimen showed that tumor cells were single or in loosely aggregated clusters.
Some cells were seen in naked nuclei-like appearance and the others were seen with abundant granular cytoplasm. There was no malignant tumor diathesis.
Nuclei were round to spindle with finely granular chromatin and relatively severe anisokaryosis but mitotic figures were absent. Histological examination revealed that the tumor had organoid arrangement of chief cells in “Zellballen” with neuron specific enolase-positive immunohistochemically.
Ultrastructural study showed numerous membrane bound neurosecretory granules in the tumor cells. Carotid body paraganglioma is rare tumor, but for the increasing use of aspiration cytology of neck tumors cytologists will see this tumor with increasing frequency.View full abstractDownload PDF (9253K) -
Toshiro KAWAI, Naohisa TSUNODA, Sachiko KUBONO, Tatsuya SAITO, Ken SAI ...1988 Volume 27 Issue 4 Pages 564-570
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThree cases of thymic carcinoid were reported. A preoperative diagnosis was established by aspiration cytology in one case. Imprint smears were obtained from operation materials in remaining two cases. Cytologically, tumor cells showed same cytological characteristics as those of carcinoids arising in other sites. They had round to oval nuclei with little pleomorphism, finely granular chromatin, relativelyabundant pale cytoplasms and sheet or rossette structure. Histologically, all tumors belonged to atypical carcinoid characterized by “Ball ” structure. Electron microscopic studies revealed neurosecretory granules in all cases, although argyrophilic reaction was positive only in one case. Metastasis was revealed in two cases and one case died of metastasis 9years after the appearance of SVC syndrome.View full abstractDownload PDF (16298K) -
Noboru FUKUNAGA, Noriaki KAMEDA, Akira FUJIOKA, Toshie SHIMOZEKI, Haru ...1988 Volume 27 Issue 4 Pages 571-578
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA 49-year-old female noticed a small, non-painful nodule below the left nipple. There was no history of trauma or inflammation in this area.
Mammographic and ultrasonic investigations revealed a nodule of 1.0cm in diameter with a dumbell-shaped calcification.
Aspiration cytology showed scattered and clustered atypical cells on Pap. smears, suggesting a scirrhous type carcinoma. The patient received atypical mastectomy with resection of regional lymph nodes.
Pathological study of the resected breast showed a fairly well demarcated, gritty, white nodule, which was characterized by a zonal arrangement of atypical osteoid, chondroid and fibrosarcomatous tissue, resembling so-called myositis ossificans, although the cells comprising the tumor were atypical and pleomorphic.
A detailed analysis of the previous cytosmears further illustrated the presence of a few different cell types. Some were found to have irregular cell boundary, eccentric, round to oval nuclei with coarse chromatin clumps and condensed nuclear membrane, which were compatible with those of osteoid cells. Others were those of chondroid with round, concentric nuclei with fine chromatin granules and smooth, thin nuclear membrane. Occasional vacuoles were included in their cytoplasm. In addition, some atypical multinuclear giant cells were also encountered.
Ultrastructural investigation of these cells demonstrated more details of cellular structures and characteristics.View full abstractDownload PDF (11101K) -
Fumio MURAYAMA, Eiichi AKAOGI, Yumiko MURAYAMA, Masataka ONIZUKA, Haru ...1988 Volume 27 Issue 4 Pages 579-583
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThe cytological manifestations of a pulmonary blastoma in the left lung of a 68-year-old male are reported. Before operation various kinds of tumor cells were obtained by fine lung needle aspiration. They showed epithelial and mesenchymal differentiation. Some epithelial tumor cells which resembled adenocarcinoma cells had foamy cytoplasm and a round nucleus. Other cells that had on irregular nucleus and scanty cytoplasm seemed to be large cell carcinoma cells. Mesenchymal elements were also various. Those that had an elliptic nucleus and fibrous cytoplasm with a stream-like arrangement were similar to fibroblasts. Those that had a spindle-shaped nucleus and irregular foamy cytoplasm were considered malignant. The cytological features of each group were compatible with the histology of pulmonary blastoma. Pulmonary blastoma seems to be difficult to diagnose diagnose from cytological examination alone. When both carcinomatous and sarcomatous elements are observed in one cytological specimen, it is necessary to consider pulmonary blastoma as a differential diagnosis.View full abstractDownload PDF (10759K) -
an autopsy case reportKyoko HARA, Masayuki YAMAUCHI, Takako KAMEI, Yoko YAMAMOTO, Syosuke MO ...1988 Volume 27 Issue 4 Pages 584-588
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA 75-year-old man presented with cough and epigastralgia. A chest X-ray showed a mass in the lower lobe of the right lung. Bronchial brushing specimens showed tumor cells which had relatively abundant cytoplasm and oval nuclei with one to three prominent nucleoli. The cytological diagnosis was class V and large cell carcinoma was suspected.
At autopsy a solid tumor (10×10×7 cm) with sharply circumscribed edges was found to occupied most of the lower lobe of the right lung. The cut surface was yellowish white and had scattered hemorrhagic and necrotic areas. The histological diagnosis was pulmonary blastoma because the tumor consisted of glandular epithelial elements, immature sarcomatous cells, skeletal muscle cells and chondroid cells.View full abstractDownload PDF (8029K) -
Double primary cancer consisting of occult lung cancer and carcinoma in adenoma of the sigmoid colonHiroyuki MIURA, Harubumi KATO, Yoshihiro HAYATA, Reiko MIURA, Akiko YO ...1988 Volume 27 Issue 4 Pages 589-592
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA 60 year-old male was admitted to another hospital on the suspicious of tuberculosis of the lung because of pleural effusion. Though no sign of tuberculosis was detected from sputum and pleural effusion, sputum cytology revealed class V, squamous cell carcinoma. After he was reffered to us, no further sign of cancer was obtained from sputum cytology or bronchoscopic examination except of the sputum cytology at the first time.
The sputum cytology after 11 months since his first examination was positive, and the 6th bronchofiberscopic examination revealed polypoid tumor located at the orifice of B5a, but without any abnormal shadow at that portion on the chest X-ray film. Left upper lobectomy was performed and the pathological findings showed pT1NOMO, stage I, squamous cell carcinoma. During follow-up, barium enema and colon fi berscopic examination were performed because of bloody stool and polypectomy was performed for polyps located in the sigmoid colon. The pathological examination revealed carcinoma in adenoma.
In cases of occult lung cancer, the frequency of multiple lung cancer or synchronous or metachronous cancer of other organs was high. So we must follow up carefully by sputum cytology and bronchofiberscopic examination and be on the look-out for subtle signs of cancer of other organs.View full abstractDownload PDF (6819K) -
Tei-ichi MOTOYAMA, Noriko ISHIHARA, Ken-ichi FUJITA, Ken-ichi TOBITA1988 Volume 27 Issue 4 Pages 593-601
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSEach three subtype of Merkel cell carcinoma, trabecular type, intermediate cell type and small cell type was studied in histological, cytomorphological, ultrastructural and immunohistochemical aspects.
Cytologic examinations were more easy and more reasonable than histologic examinations in the subclassification of tumors based on cell size. We would like to propose a tentative plan as follows: When the slide stained by Papanicolaou's method shows that the tumor cells with large nucleus more than 10μ in diameter count for more than 70% of all tumor cells, the tumor should be classified as a trabecular type. When the tumor cells with small nucleus less than 7.5μ in diameter comprise more than 50%, the tumor should be named small cell type.
Although electron microscopy is necessary for a definitive diagnosis of Merkel cell carcinoma, it is not always easy to detect neurosecretory-like granules. Immunohistochemistry for neuron-specific enolase (NSE) is very helpful to screen such tumors. NSE in Merkel cell carcinomas was markedly decreased in immunoreactivity by formalin fixation, while ethanol fixation for cytologic examination did not significantly influence the NSE immunoreactivity. Our data indicate that cytologic specimens are appropriate to NSE immunostaining.View full abstractDownload PDF (14252K) -
Keizo FUKUMA, Hidetaka KATABUCHI, Shunichi FUJISAKI, Hitoshi OKAMURA, ...1988 Volume 27 Issue 4 Pages 602-603
Published: July 22, 1988
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (3298K)
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