Young unripe persimmon fruit is a waste material and environmental burden. We studied whether fermentation using
Aspergillus awamori and
Lactococcus lactis subsp.
Lactis would be effective for utilization of immature
Diospyros kaki ‘Fuyu’ fruit. These microorganisms caused an increase of inhibitory activity against β-lipase. An aqueous fraction of material fermented with
A. awamori showed the highest activity against β-lipase (IC
50=16 μg/mL) at day 7, whereas an aqueous fraction of material fermented with
L. lactis showed the highest anti-β-lipase activity (IC
50=29 μg/mL) at day 14. Additionally, material fermented with both
A. awamori and
L. lactis showed increased activity against angiotensin converting enzyme. The fruit of ‘Fuyu’ contains sucrose and citrulline, and these compounds disappeared by day 7 of fermentation. The fermentation products of
A. awamori differed from those of
L. lactis; mannitol was identified from the former, but not from the latter. These results indicate that
A. awamori and
L. lactis produce different compounds that are involved in inhibitory activities. Therefore, fermentation of immature fruit of the persimmon variety ‘Fuyu’ using
A. awamori and
L. lactis increases its functionality, suggesting that such immature persimmon could be utilized as a new material for functional foods.
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