We have already developed the electrical sterilization method with the metal coating hollow fiber membrane. The sterilization mechanism was imagined as follows : the leakage of intracellular contents was confirmed when bacteria were sterilized by a direct electric current. This leakage proved indirectly that the cell membrane was partially broken.
In this paper, the leakage phenomenon and the mechanism on the sterilization were focused and the possibility whether the metal coating hollow fiber membrane became a new device of gene extraction was examined. After the direct electric current to the membrane surface trapped
Escherichia coli O 157 was passed, the gene was checked. As a result, the vero toxin type 1-gene could be detected. The detection sensitivity on the gene extraction was mainly depended on the concentration of sodium dodecyl sulfate (SDS) which was an anion surfactant and the value of the direct electric current. The highest sensitivity in the extraction was obtained under 5 % SDS and 300 mA. In addition, the gene was extracted under
Escherichia coli O 157 of 10
7or 10
8CFU/m
l contained in other bacteria of 10
8CFU/m
l.
This system needed the time to extract the gene only in 10 minutes. The system was also simpler equipment which consists of the perista-pump and the power supply except the membrane.
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