巻号一覧
43 巻 (2018)
- 6 号 p. 252-
- 5 号 p. 194-
- 4 号 p. 122-
- 3 号 p. 80-
- 2 号 p. 34-
- 1 号 p. 2-
43 巻, 2 号
選択された号の論文の8件中1～8を表示しています
- |<
- <
- 1
- >
- >|
巻頭言
特集 : 医療現場で活躍する膜様組織－細胞シートと生体膜－
-
堺 裕輔, 江口 晋2018 年 43 巻 2 号 p. 34-39
発行日: 2018年
公開日: 2018/04/05
ジャーナル オープンアクセスTreatment of digestive organs with cell sheets is widely performed from basic research to clinical application. The treatment strategy greatly differs between the gastrointestinal tract (e.g. esophagus, stomach, and intestine) or other digestive organs (e.g. liver and pancreas). For the esophagus and small intestine, rapid tissue repair was performed by the cell sheet transplantation to prevent postoperative strictures and perforation. The tissue repair processes were well controlled by cytokine and collagen productions from the cell sheets. Sixty percent of patients who underwent complete circular or semicircular endoscopic submucosal dissection (ESD) did not cause postoperative strictures due to endoscopic transplantation of autologous oral epithelial cell sheets that had been transported by air. On the other hand, regenerative treatment for the liver and pancreas is underway from an approach based on organ fabrication. In order to carry out safe and rapid liver regenerative treatment, we developed engineered hepatocyte/fibroblast sheets (EHFSs) that were composed of two layers of fibroblasts and hepatocytes. The EHFSs developed into functional vascularized subcutaneous human liver tissues (VSLTs) in the mouse. These regenerative treatments using cell sheets will definitely be one of the treatment options in the future.抄録全体を表示PDF形式でダウンロード (939K) -
松本 卓子, 神崎 正人2018 年 43 巻 2 号 p. 40-43
発行日: 2018年
公開日: 2018/04/05
ジャーナル オープンアクセスThe pleura which is membrane is covering of thoracic cavity. A defect or injury of visceral pleura causes air leakage from lung parenchyma, the presence of air in the thoracic cavity lead to lung collapse. Several methods including the use of various sealants are ineffective in the closure of air leaks (ALs), and various surgical techniques result in pleural adhesion which is negative consequences. We previously reported a novel method of cell–sheet engineering of lung AL sealants using cell sheets harvested from temperature–responsive culture dishes. Tissue engineered cell sheets is closing ALs, respond according to the dynamic movements of the lungs during respiration, and avoids pleural adhesion. Additionally, host mesothelial cells migrate to cover the transplanted cells sheets. Therefore, tissue engineered cell sheets are expected to become new material for therapy of pleural defect or injury.抄録全体を表示PDF形式でダウンロード (927K) -
澤 芳樹2018 年 43 巻 2 号 p. 44-49
発行日: 2018年
公開日: 2018/04/05
ジャーナル オープンアクセスCellular sheet transplantation is a fundamental technique of regenerative medicine, which is widely applicable to the whole cellular sources including skeletal myoblasts, cardiac myocytes, embryonic stem cells (ES) and induced pluripotent stem cells (iPS). In 2007, this author succeeded withdrawal from left ventricular assist system (LVAS) in a patient with severe dilated cardiomyopathy requiring cardiac transplantation by using this cellular sheet transplantation, and confirmed that this patient is fine at present. After obtaining this clinical evidence of the first successful case in human, more than 50 cases of end–stage heart failure were treated with this technique, and two of them were capable of rehabilitation at home without any support of LVAS. This line of evidence verified clinically that cellular sheet transplantation technique is a safe and effective treatment strategy for advanced heart failure by improving symptoms, cardiac function and life prognosis. According to the technological diffusion, a health insurance publication was approved after completing the company clinical trials with respect to the cellular sheet transplantation applied to the patients with severe ischemic cardiomyopathy. Furthermore, this author commenced two doctor–oriented clinical trials concerning the cellular sheet transplantation for adults and children with idiopathic dilated cardiomyopathy, respectively.抄録全体を表示PDF形式でダウンロード (857K) -
前原 美樹, 豊田 恵利子, 高橋 匠, 佐藤 正人2018 年 43 巻 2 号 p. 50-55
発行日: 2018年
公開日: 2018/04/05
ジャーナル オープンアクセスWe are undergoing clinical research for regenerative medicine using chondrocyte sheets for the treatment of articular cartilage. We previously concluded a successful clinical study using autologous chondrocyte sheets and are now conducting our second clinical study using allogeneic chondrocyte sheets. Currently, allogeneic chondrocyte sheets are fabricated from chondrocytes derived from the surgical remains of juvenile polydactyly patients, which show promise as a new cell source. The use of allogeneic chondrocytes allows the fabrication of chondrocyte sheets for transplantation from those cells whose safety and quality have been established prior to fabrication. However, to further accelerate joint treatment with chondrocyte sheets, the development of a practical system for storing a readymade supply of chondrocyte sheets, i.e. a preservation system, is indispensable from the viewpoint of cost reduction and stable supply. Here, we report the development of a vitrification method for chondrocyte sheets that allow the long–term cryopreservation of transplantation–ready chondrocyte sheets. This technology will accelerate the popularization of chondrocyte sheets used in articular cartilage regeneration.抄録全体を表示PDF形式でダウンロード (975K) -
鬼塚 理, 岩田 隆紀2018 年 43 巻 2 号 p. 56-62
発行日: 2018年
公開日: 2018/04/05
ジャーナル オープンアクセスPeriodontitis is a chronic inflammatory disorder that causes deterioration of the periodontal tissues that support teeth. Several biomaterials, such as bone graft materials, barrier membranes and protein products, have been developed and used clinically to repair periodontal tissue defects clinically. However, it is difficult to regenerate the complete periodontal tissue structure. Recently, regenerative therapies using multipotent mesenchymal stromal cells (MSC) have been introduced to overcome the limitation of conventional procedures. These cytotherapies have been shown to be safe and effective for the treatment of periodontal tissue defects. In order to achieve more effective treatment, our strategy has been to integrate this stem cell biology and cell sheet engineering. This cell sheet technology has already been employed in clinical trials (one of these has already approved), confirming the safety and efficacy of the treatment method. In this review, we introduce recent progress in the engineering of cell sheets and review the potential of cell sheet technology for periodontal regenerative medicine.抄録全体を表示PDF形式でダウンロード (800K) -
武田 志津2018 年 43 巻 2 号 p. 63-66
発行日: 2018年
公開日: 2018/04/05
ジャーナル オープンアクセスGreat hopes are being placed on regenerative medicine providing the next generation of therapies that can treat patients with incurable disease. At present, culturing of cells for regenerative medicine is mainly performed manually. Automated culture of cells for medical use is the greatest challenge in safe and large–scale production toward wider adoption of regenerative medicine. The closed systems for automated culture equipment developed by Hitachi can produce cell sheets with quality equivalent to that of manual culture. Hitachi has also developed cell sheet transportation container that can maintain sterility, constant temperature and pressure. These researches and developments can facilitate the spread of regenerative medicine to deliver high–quality cells to patients around the world.抄録全体を表示PDF形式でダウンロード (890K)
原著
-
Ting Wu, Kazuhiro Tanaka, Xiangshu Chen, Izumi Kumakiri, Hidetoshi Kit ...2018 年 43 巻 2 号 p. 67-73
発行日: 2018年
公開日: 2018/04/05
ジャーナル フリーAEI–type AlPO–18 zeolite membranes were prepared on macroporous mullite and two–layer α– Al2O3 tubes with less expensive N, N–diisopropylethylamine (DIPEA) as a single template. The obtained membranes by single DIPEA template showed both high CO2/CH4 and N2/CH4 perm–selectivities and fluxes. The membrane prepared on two–layer α– Al2O3 support showed a CO2 single gas permeance as high as 4.2 × 10–6 mol m–2 s–1 Pa–1 with the perm–selectivity of 90 for CO2/CH4 at 298 K and 0.11 MPa. Besides, a compatible N2/CH4 perm–selectivity of 8.1with N2 single gas permeance of 1.5 × 10–7 mol m–2 s–1 Pa–1 at 298 K and 0.11 MPa was obtained from the membrane prepared on macroporous mullite support. Although a few of AFI type AlPO–5 impurities were formed on the surface of those AlPO–18 zeolite membranes, the AFI impurities didn’t lower the gas permeation performance.抄録全体を表示PDF形式でダウンロード (713K)
製品 & 技術
-
森本 昌文2018 年 43 巻 2 号 p. 74-77
発行日: 2018年
公開日: 2018/04/05
ジャーナル 認証ありIn the gas separation process using a membrane, it is important to quantify the microporous structure (pore size, pore volume, specific surface area, etc.) of the membrane. In addition, most of the separation process is a dynamic process, and it is not necessarily said that its performance depends on the structure of the membrane. Differences in the diffusion coefficient of guest molecules may play an important role in separation performance, or mixed gas always exists in the separation process and the selection system of mixed gas may have an important influence on membrane separation performance. In order to evaluate the nanoporous structure, gas adsorption measuring apparatuses are commercially available and used. Many commercially available gas adsorption apparatuses measure highpurity nitrogen, argon, carbon dioxide, etc. to evaluate the pore structure and it was impossible to evaluate the dynamic process. Evaluation of dynamic process using mixed gas is a situation where users are experimenting with the system which they made their own. In this paper, we introduce the outline of dynaSorb BT, a multicomponent gas adsorption apparatus using the dynamic flow method in commercial equipment.抄録全体を表示PDF形式でダウンロード (729K)
編集後記
- |<
- <
- 1
- >
- >|