巻号一覧
8 巻 (1983)
- 6 号 p. 328-
- 5 号 p. 258-
- 4 号 p. 194-
- 3 号 p. 130-
- 2 号 p. 66-
- 1 号 p. 2-
8 巻, 5 号
選択された号の論文の8件中1～8を表示しています
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矢島 由紀子, 斉藤 寿一1983 年 8 巻 5 号 p. 258-267
発行日: 1983/09/01
公開日: 2011/03/04
ジャーナル フリーThe structural requirement for full biological activity of parathyroid hormone (PTH), on 84-amino acid single-chain polypetide is satisfied by the 34-amino acid fragment at its NH2-terminal. The procedure to obtain a radioligand with both high specific radioactivity and bioactivity includes a gentle iodination using electrolysis or lactoperoxidase. The sulfur-free analogue [Nle8, Nle18, Tyr34] bPTH (1-34) amide, is stable during the oxidation and is also used as a radioligand. The PTH receptor in plasma membrane of chicken renal cortex is a single component with high affinity to bPTH (1-34), (Kd=7 to 10nM). Guanyl nucleotides stimulate not only PTH dependent adenylate cyclase activity but also the dissociation of 125I-bPTH (1-34) from a plasma membrane of canine renal cortex.
Pseudohypoparathyroidism Type 1 (PHP-1) is an inherited disease characterized by its resistance to stimulation of adenylate cyclase by PTH. The erythrocytes of patients with PHP-1 show partially deficient activity of a guanyl nucleotide regulatory protein that is required for a functional coupling of hormone receptors and catalytic adenylate cyclase. It is also reported, however, that some of the clinically evident PHP-1 patients are possessed of quantitatively and qualitatively normal N-protein activities in erythrocytes. These findings suggest the biochemical and genetic heterogeneity of the disorder classified as PHP-1.抄録全体を表示PDF形式でダウンロード (1422K) -
筋小胞体ベシクルの研究から布垣 一幾, 葛西 道生1983 年 8 巻 5 号 p. 268-277
発行日: 1983/09/01
公開日: 2011/03/04
ジャーナル フリーApplication of four fluorescence techniques to the measurement of the passive ionic permeability in biomembrane was demonstrated with the examples in the investigation of sarcoplasmic reticulum vesicles, in which fluorescence technique has widely been employed as well as other techniques, e. g., radio isotope tracer method, volume change-light scattering method, and conductance measurement using artifitial planar bilayer membrane.
First, the chlortetracycline method was described. The change in the amount of the intravesicular divalent cations can be followed by measuring the fluorescence of the dye, which increases upon binding to diamagnetic cations such as Ca2+ and Mg2+ on membrane surface. Secondly explained was the technique using anilinonaphthalenesulfonate, whose fluorescence change arises from its binding to the membrane that depends on the membrane surface potential, and indicates the change in the ionic compositions outside and inside the vesicles. Thirdly, application of the slow response of the potential-sensitive dyes was described. Permeability of ions can be determined from the amplitude and its dissipation rate of transmembrane diffusion potential due to ionic concentration gradient. Lastly, the fluorescence quenching method was shown to give the highest time resolution. The decrease of the fluorescence of the water soluble dyes entrapped in the vesicles due to the influx of ionic quenchers is analyzed to yield the ionic permeability of the vesicles.抄録全体を表示PDF形式でダウンロード (1419K) -
野中 勉1983 年 8 巻 5 号 p. 278-286
発行日: 1983/09/01
公開日: 2011/03/04
ジャーナル フリーThis review is organized under the following headings with 79 references.
1. Preface-General surveys
3. Preparative methods, kinds, structures, and characters of the polymer-coated electrodes
2.1 Preparative methods
2.2 Kinds and structures
2.3 Characters
3. Electrode processes at the Polymer-coated electrodes
4. Applications of the polymer-coated electrodes
4.1 Electrolyses a. Inorganic b. Organic
4.2 Cells
4.3 Analytical chemistry a. Electrode kinetics b. Reference electrodes c. Potentiometry and pH sensors
4.4 Others a. Electrochromism-display devices b. Electroluminescence c. Photoresponse
5. Conclusion
References抄録全体を表示PDF形式でダウンロード (1347K) -
矢原 一郎1983 年 8 巻 5 号 p. 287-295
発行日: 1983/09/01
公開日: 2011/03/04
ジャーナル フリーSeveral topics on cytoskeletal structures are discussed particularly in relation to dynamics of the plasma membrane based on our recent observations. The mobility of surface receptors of lymphocytes is both positively and negatively regulated by cytoskeleton. Conversely, surface events induced by ligands caused reorganization of cytoskeletal structures which consist of microfilaments and microtubules. A study on ligand-independent capping induced in hypertonic medium disclosed a problem as to how suprastructures of filamentous actin are disorganized and reorganized during surface capping. The mechanism of the actoin of cytochalasin, a probe for elucidating the function of microfilaments, is presented. Finally, we demonstrate a cell-cycle dependent alteration of microtubule structures of mouse lymphoma cells.抄録全体を表示PDF形式でダウンロード (2118K) -
Manabu Igawa, Taka-aki Tachibana, Masao Tanaka, Manbu Sew1983 年 8 巻 5 号 p. 297-299
発行日: 1983/09/01
公開日: 2011/03/04
ジャーナル フリーA circuit current was effectively generated through a macro mosaic membrane, which was composed of separated membranes of a cation exchange membrane and an anion exchange membrane. Under a concentration gradient of non-electrolyte, up-hill transport of potassium chloride was observed with this macro mosaic membrane.抄録全体を表示PDF形式でダウンロード (297K) -
Masayuki Nakagaki, Ryosuke Takagi1983 年 8 巻 5 号 p. 301-310
発行日: 1983/09/01
公開日: 2011/03/04
ジャーナル フリーExcitation of membranes is usually interpreted by two kinds of theory. One of them is the change of permeability coefficient of Hodgkin & Katz. The other is the opening and closing of ionic channels. If these two theories are to be compatible, the permeability coefficient of Hodgkin & Katz ought to be correlated with the opening and closing of ionic channels. In this paper, the correlation between them are theoretically discussed and a study of the excitation of membrane is developed.
As the result, it is pointed out that the Goldman-Hodgkin-Katz equation fails to explain the experimental results on the excitation of membrane (for example, excitation of squid giant axon), if the membrane model which is usually used as the theoretical model since Teorell and Meyer & Sievers, bulk soln. //membrane// bulk soln., is used as the theoretical model. We, therefore, propose a sublayer model of membrane, bulk soln. /sublayer//membrane//sublayer/bulk soln., together with the following assumptions : i) The membrane has ionic channels. ii) The membrane has electric charge and its density is θM (algebraic). iii) A Donnan equilibrium condition is set up between the sublayer and the bulk solution. iV) Ions in the sublayer are adsorbed on the surface of specific ionic channels and permeate through these channels. V) The electric potential in the sublayer is equal to that at the membrane surface.
On the basis of this sublayer model, we conclude that the permeability coefficient of Hodgkin & Katz is related to the number of open ionic channels, so that the permeability coefficient changes with opening and closing of the ionic channel.抄録全体を表示PDF形式でダウンロード (1116K) -
Xie-qing Xu1983 年 8 巻 5 号 p. 311-314
発行日: 1983/09/01
公開日: 2011/03/04
ジャーナル フリーThe permeation and retention characteristics of polysulfone resin membranes in separation of protein composition from its aqueous solution were investigated by changing the additive reagents of the casting solution of membranes. These reagents were the polyethylene glycol (PEG), polypropylene glycol (PPG), polyvinyl pyrrolidone (PVP) and ethoxy ethyl acetate (EEA). The value of water permeability were influenced remarkably by the additive reagents.抄録全体を表示PDF形式でダウンロード (362K) -
上釜 兼人, 入江 徹美, 小田切 優樹, 星野 輝彦, 山田 義次, 大谷 淑郎1983 年 8 巻 5 号 p. 315-321
発行日: 1983/09/01
公開日: 2011/03/04
ジャーナル フリーThe protective mechanism of cyclodextrins (α-, β-, and γ-CyDs) on the imipramine (IMP) -induced hemolysis was discussed on the basis of inclusion complexation. Inclusion complexation of IMP with CyDs in isotonic solution was assessed by ultraviolet and circular dichroism spectroscopies. CyDs were found to protect the human erythrocytes from the hemolysis and shape changes induced with IMP, depending upon the magnitude of stability constant of IMP-CyD complexes (β->γ->α-CyD). The uptake of IMP into erythrocytes was also reduced by the addition of CyDs in the order of, β->γ->α-CyD. The affinity of the complexed form of IMP to erythrocytes was proven to be negligibly small compared with that of the free IMP. The poor affinity of the complex can be explained by the decrease in surface activity and/or membrane permeability of IMP by encapsulation in hydrophilic and bulky CyD molecule. These results suggest that the protective effect of CyDs on the IMP-induced hemolysis is mainly caused by the reduction in the effective hemolytic concentration of IMP through inclusion complexation rather than the stabilizing action of CyDs on the erythrocyte membrane.抄録全体を表示PDF形式でダウンロード (1227K)
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