Twelve male rats were individually housed in separate metabolic cages. Body weight and urinary catecholamines of the rats were measured during nine days of the isolation. Mean body weight of the rats decreased soon after transfered into the metabolic cages and thereafter increased steadily. Mean urinary level of ad-renaline was high during the early days of the isolation. The rats could be classified into two groups according to their body weight changes at the early stage of the isolation; one group showed a decrease in the body weight and the other showed a steady increase in the weight. The group with loss of the body weight showed a higher level of urinary adrenaline in the early days of isolation than that of the group with no weight loss. These results may suggest that the new environments involved in the individual caging were stressful to most of the isolated rats, and that there was an individual difference in the susceptibility to the isolation stress.
The purpose of this study was to investigate relation between skin temperatures and plethysmograms, in addition to differencs of skin temperatures between sexes. Subjects consisted of 80 male and 276 female office workers. Thermistors of Bio-electric Thermographic Computor placed on the forehead, right 3rd ventral fingertip and plantar toe in the room, where ambient temperatures were adjusted to approx-imately 23.0°C. The data indicate that average finger skin temperature of male workers was significantly higher than that of female workers. On the contrary, average toe skin temperature was 25.0°C in the former and 26.0°C in the latter. One explanation stemmed from the fact that young female workers put on their boots outdoor in winter. While, among female workers, the group of Type 1 digital plethysmogram presnted normal skin temperatures of finger and toe as compared to Type V or VI group, which contained a large proportion of those with less than 25.0°C as the finger skin temperture. Moreover, in screenings of peripheral blood circulation, the standard values are considered to be 27.028.0°C for the female finger skin temperature, and 67°C for the difference between the forehead and the finger skin tempertures.
A metallothionein-like protein was identified from a mono-cellular organism Tetra-hymena pyriformis after incubation of organisms in the medium containing cadmium. Sulfhydryl group (SH) contents in the fractionated metallothionein-like protein in-creased up to twenty-four times of the control after exposure to cadmium. The increase of the protein was significantly correlated with the cadmium concentration in the medium (0.97). Molecular weight of the protein was estimated to be 59, 000 by sodium dodecyl sulfate gel electrophoresis and incorporation of 35S-cysteine into the protein. Zinc or calcium added in the medium showed no effect on the amounts of metallothionein-like proteins induced by cadmium exposure. Increase of SH group content as a biological response to the cadmium exposure might be a common phenomenon observed in a variety of animal species.
Effect of manganese administration, on the morphology and metal concentration in various tissues of rats fed 10% casein diet, were investigated. Daily intra-peritoneal administration of MnC12•H2O (15 mg/kg) to the rats fed 10% casein diet for a period of 45 days produced almost identical increase in metal concentration of various tissues as after similar treatment to the animals fed basal diet. Protein deficient diet did not alter the extent of manganese induced morphological lesions in various tissues of rats. The data indicated that 10% casein diet to the adult rats did not make them more susceptible to manganese poisoning.
A method to construct a permeation tube of vinyl chloride was described. The constructed tube was held in a water jacket, temperature of which was controlled by circulating water from a thermoregulated water bath, and a clean air diluent flew through the jacket to prepare air-gas mixture stream of known concentration. The concentration of vinyl chloride in the stream was determined gas chromato-graphically and a fairly good linearity was observed between the calculated con-centration and chromatographic peak height. An apparent activation energy of permeation was calculated from temperature dependency of permeation rate.