A simple analytical method was developed to determine
p-nitrochlorobenzene (
p-NCB) in working environmental air by reversed-phase high-performance liquid chromatography (HPLC). Nitrobenzene, nitrotoluene isomers, and NCB isomers were separated and
p-NCB was determined by reversed-phase HPLC as follows: column; Hitachi gel #3056 (4.0mm I.D. x 150mm), mobile phase; 2-propanol: water (40:60, v/ v) with a flow rate of 0.8ml/min, column temperature; 50°C, detector; UV detector at 270nm. Nitrobenzene,
o-,
p-nitrotoluene, and
o-,
m-NCB were separated from
p-NCB, but
m-nitrotoluene could not be completely separated from
p-NCB. The limit of detection (signal-to-noise ratio of 3:1) of
p-NCB was 0.3ng. Repeatability of the HPLC method was excellent. The HPLC method enabled to determine one-tenth of the administrative level (1mg/m
3) of
p-NCB, since the lower limit of determination was 0.05mg/m
3. Therefore, the present HPLC method proved to be applicable to the determination of
p-NCB in working environmental air.
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