Based on a possibility of the phase separation of methane fermentation connected the two fermenters in series which had different hydraulic retention times, the experiment of the two-phase methane fermentation was performed with the substrate of the mixture of swine feces, and swine feces and urine. As the results, it became possible to charge higher loadings of organic matter than that of the former conventional methane fermentation, and digested gas production rate was maintained high as the digested gas yield was increased by 20-30%. It was found that the loading of organic matter (Lvs) of 7.5kg-vs/m
3/d was the optimum for the purpose of recovering the energy, and the Lvs of 5.0kg-vs/m
3/d was optimum operation condition in case of considering for the quality of digested liquid, such as the ratio of volatile solid removal, volatile acid removal, and NH
4+. In addition, each methane gas production rate is 1.596, 1, 447Nm
3-CH4/m
3/d, respectively.
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