Outbreak of animal infectious diseases damages not only animal production but also the entire regional economy. It is necessary to raise the level of biosecurity in animal farms as to produce safe animal products. And It is necessary to reduce microbe concentration in livestock houses as much as possible in order to reduce disease risk. Therefore, the present study was carried out to elucidate the relationship between aerosol concentration and airborne microbe concentration in enclosed weaning pig houses in order to aim to develop estimation method of airborne microbe concentration with aerosol that can be measured in real time as a index. In addition, we established the gene quantification analysis of PCV2 and PRRSV, we examine the relation of pathogenic microorganisms concentration and aerosol concentration. As a result, the strong correlation between aerosol concentration and viable airborne microbe concentration was found in the aerosol particle size more than 5.0 μm. And, as for pathogenic microorganism concentration, PCV2 was below the detection limit even in the specimen either during the measurement period. However, PRRSV was detected 0.132 TCID
50/m
3 from air samples and 0.138 TCID
50/m
3 from oral fluid in October 3 when aerosol concentration was the highest during measurement period. It was examined that there were some correlation between aerosol concentration and PRRSV concentration.
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