In this report, the examples of the development of the region using the Ecosystem of Satoyama, Satochi and Satoumi have been shown.
In Satoyama, the forest resources are degrading due to the decline in working population and the maintenance needs of the forest are not being met. Some of the methods used to prevent the degradation includes encouraging and facilitating the children and grandchildren’s return to the region. Facilitating the exchange of people from urban area who are interested in utilising the ecosystem is found to be useful in the development as well.
In Satochi area, increasing number of farmland is being abandoned because of aging population among farmers and lack of successors in the region. In order to maintain Satochi and its ecosystem, it is critical to develop eco-friendly agriculture and to develop a network of farmers or group farming system that can develop regional farming in sustainable manner.
As for Satoumi of Tohoku region （where it was severely damaged by the Great East Japan Earthquake in 2011） it is critical to re-develop marine industry, that includes fisheries and marine product processing industry, which can support the reinvigoration of the fisheries industry as a whole. Current agendas are to overcome shortage of labour and re-development of the fishery product market outlet. Agendas in the medium to long term include developing attract regional products and to increase the interaction of people by utilising the regional educational facilities and functions.
For the removal of boron in liquid, N-methylglucamine （NMG） as a chelating moiety was immobilized to the poly-glycidyl methacrylate chain grafted onto a rayon fiber irradiated by means of electron beam. The density of the NMG immobilized was 1.9 mmol/g of the product. The resultant fiber was compared to a 6-nylon-based chelating fiber with an NMG density of 1.9 mmol/g that was derived from an electron-beam-irradiated 6-nylon fiber. The equilibrium binding capacity agreed with both fibers at 15 mg-B/g. The dynamic binding capacities of the rayon -and 6-nylon-based chelating fibers for boron were 9.4 and 4.5 mg-B/g, respectively, determined from respective breakthrough curves at a space velocity of 40 h－1 of 150 mg-B/L solution flowing through a 10 mm-high fiber-packed bed.
The particles of microscopic ice （Nano-ice : NI） produced by nanoICE machines using molecular ice technology are less than one micron in size. Nano-ice has greater heat-uptake surface area and therefore induces chilling faster than conventional ice methods. This study examined the effect of NI storage on several freshness indicators for Japanese horse mackerel, Trachurus japonicus, compared with sea water ice （SI） or refrigerated （R） storage. Fish were obtained at the fishing port of Onagawa-cho in Miyagi prefecture. In SI storage, a higher C-value for the abdominal skin and a lower K value for the dorsal common muscle were present compared to those in R storage, though lowered a- and C-values for the red muscle were found. Nano-ice storage showed higher L-values for the dorsal skin and higher C-values for the abdominal skin, while reducing the increase in K value for the dorsal common muscle. Lowered levels of volatile basic nitrogen were found in NI storage on day 1. On day 7, the NI storage group had lower peroxide values than the SI and R storage groups. In early stages of storage, NI treatment also showed a tendency for a decrease in the number of bacteria attached to the gill surfaces. These results indicate that storage in Nano-ice has the effect of suppressing an increase in K values and lipid peroxidation on the dorsal common muscle, as well as maintaining fresh skin and red muscle color of the Japanese horse mackerel. It can be concluded that Nano-ice used for fish storage was effective in maintaining high quality and high freshness of the fish.
A gene for halophilic thioredoxin （TrxA） was isolated from extreme halophile, Halobacterium sp. NRC-1. The TrxA was highly soluble with or without high salt concentrations. Circular dichroism and thiol-disulfide oxidoreductase activity demonstrated that TrxA unfolded at low salt concentrations, and folded and was active at high salt concentrations. Folding/unfolding of TrxA was clearly salt dependent, and highly reversible. This halophilic TrxA was employed to develop a solubility-enhancing partner protein for the expression of fusion proteins. TrxA-amylase fusion protein was expressed in Escherichia coli in soluble form with a～ 2.5-fold higher amount than the β-lactamase-amylase fusion protein previously reported.