All issues
Volume 14, Issue 1
Displaying 1-5 of 5 articles from this issue
- |<
- <
- 1
- >
- >|
-
[in Japanese]1964 Volume 14 Issue 1 Pages 1-11
Published: February 29, 1964
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSDownload PDF (1637K) -
C. A. EVANS1964 Volume 14 Issue 1 Pages 13-25
Published: February 29, 1964
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSDownload PDF (2808K) -
Saiji YOSHII1964 Volume 14 Issue 1 Pages 27-37
Published: February 29, 1964
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSMyxovirus parainfluenzae 1 (HVJ) multiplied readily and could be passaged serially in the cells of an established strain, PS cell. The viral growth in PS cells was recognized easily by marked cytopathic changes, production of hemagglutinin, and positive cyto-hemadsorption. The infectivity titers of HVJ in PS cell were almost the same as EID50 titers, even when the egg-adapted virus was tested. It was concluded that PS cell was one of the most suitable host systems for HVJ.
The intracellular development of viral antigen in HVJ-infected PS cells was studied by the fluorescent antibody technique. Specific viral antigen was first found as fine granules in the perinuclear area. The antigen steadily increased in quantity and was observed as numerous granules distributed throughout the cytoplasm. Later, the fluorescent material began to form larger aggregates and, in some cases, such large masses occupied most of the cytoplasm. No fluorescent material was found in the nucleus at any stage of the infection. Eosinophilic intracytoplasmic inclusion bodies were found in the cells infected with HVJ. The nature of this inclusion body is now under investigation.
Viral carrier culture was obtained by prolonged cultivation of the cells which had survived HVJ-infection. The culture continued to produce virus over a period of many months. Immunofluorescent staining revealed that about 30% of cells in the culture appeared to contain viral antigen. Varying amount of viral antigen was contained in each of these cells. Thus, the cells which possessed very small amount of antigen as fine granules and the cells which contained large masses of fluorescent material were found simultaneously in the same culture. The nature of this viral carrier state was discussed in the light of the results obtained.View full abstractDownload PDF (4029K) -
Heihachi ITOH, Yoshio MORIMOTO, Haruo HASHIMOTO1964 Volume 14 Issue 1 Pages 39-44
Published: February 29, 1964
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSProcedures of the newly devised mouse potency test of inactivated poliovirus vaccine are as follows: Mice are immunized intraperitoneally with two doses of each dilution of test vaccine separated by 3 days, They are challenged intraperitoneally with a fixed large dose of each type poliovirus after 4 days of second vaccination. Approximately 6 hours after the virus challenge they are bled by heart puncture. The individual heparinized blood is frozen-stored until tested, when the thawed whole blood is inoculated undiluted into each of two monkey kidney tissue culture tubes in 0.1ml amount. The tubes are incubated at 37°C and observed for specific CPE, final reading is made on 7 th day. Under these conditions virus titer of mouse blood in the unvaccinted control group used to fall in the range of 102 to 103 TCD50per 0.1ml. For the immunized mice, when neither of the inoculated tubes shows CPE, the mouse blood is considered as virus-negative. A ratio, number of mouse virus-negative vs. number of mouse tested, is obtained for each of vaccine dilutions. To indicate potency of vaccine 50% Antigenic Extinction Limiting Value is calculated by Reed & Muench's method using above-mentioned ratios.
This mouse potency test can considerably curtail time and labor for the work. In a number of experiments including repeated tests with same lots or vaccine this test has been shown to be an acceptable rapid method measuring immunogenicity of poliovaccine, especially, for orientation purpose of a study. Discussions on the sensitivity of the test have been given from our data described.
As for lyophilization of vaccine, a freeze-dried product having good potency as can be used as a reference purpose has been prepared by concentration of vaccine using Carbowax, dialysis against hypotonic salt solution and addition of Polyvinylpyrrolidone, Glycine and L-Hydroxyproline before lyophilization. Increase of thermostability of the lyophilized vaccine as compared with the original fluid vaccine has been demonstrated for Type 1 component in the 50°C heating test.View full abstractDownload PDF (850K) -
Ryochi FUJII, Mikio MINAMITANI, Tatsuhiko SHINOZAKI, Ken NAKAMURA, Hit ...1964 Volume 14 Issue 1 Pages 45-51
Published: February 29, 1964
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSA serologic survey of RS infection by complement fixation test was conducted on 305 cases of acute respiratory illness and 76 nonrespiratory control cases at our hospital in Tokyo from May, 1960 to May, 1961, and from April, 1962 to January, 1963. Twenty cases (6.5%) were serologically diagnosed as having RS infection in the respiratory disease group. Those are the first cases of RS infection to be reported in Japan. The majority of the cases were found from September to January. RS infection was more frequent among the ward patient (10.3%) than the out-patients (6.5%).
In two cases of 79 controls inapparent infection with RS virus were found.
Multiserologic responses to certain other respiratory viruses accompanied with antibody response to RS virus were experienced in 12 cases, in which simultaneous rise to adenovirus was most frequent. The age distribution of RS complement fixing antibody among healthy children in the Tokyo area was similar to these observed in U.S.A. and indicated that a high percentage of children aquired RS infection at preschool age, the antibody incidence as high as 58% at five year of age.
The clinical signs and symptoms of RS virus infection were nothing particular from other common viral infections of the respiratory tract. Young children seemed to be liable to show low respiratory tract involvement.View full abstractDownload PDF (1512K)
- |<
- <
- 1
- >
- >|