[in Japanese]

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An outbreak including aseptic meningitis and acute febrile illness took place in a small village; Wakinosawa, Aomori Prefecture of Northern Japan, between June and August of 1964. Virological and serological studies revealed that the epidemic was caused by Echovirus type 4. Echovirus types 4 was isolated from 7 (16%) of 43 cerebrospinal fluids, 16 (35%) of 46 throat swabs and 41 (43%) of 95 faeces obtained from the patients in acute stage within 6 days after onset. Paired sera of 23 patients, 18 with aseptic meningitis and 5 with acute febrile illness, were examined for heutralizing antibody against the Du Toit strain of Echovirus type 4. In all of them, a significant rise of antibody titer was shown. However, neutralizing antibody titer obtained with the current strain (W-45-64) was much lower than that with the Du Toit strain.
Outbreaks due to Echovirus type 4 has been reported in many regions of Japan in the summer and fall of 1964 (HINUMA and OHI: Igaku no Ayumi, 51, 498, 1964) and the outbreak reported here is considered to be comprised in the wide-spread occurrence.

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1. UV-treated φx174 is reactivated upon infecting E. coli C treated with mitomycin C or with UV.
2. E. coli 15T-D3, which has some kind of inducible phage, can show same reactivation as observed in E. coli C. However, after induction of the phage, this ability disappered rapidly.
3. It is supposed that the UV-damage on the single stranded phage DNA is repaired after it becomes double stranded form (RF).

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Inhalation and intranasal spraying methods were compared in administration of live influenza vaccine to human subjects. Both methods were useful in live influenza vaccination, but with respect to economy and conveniency, inhalation method was better.
Three inhalation methods were compared. Deep breathing, urgent breathing and normal breathing in 10 seconds inhalation made no remarkable differences in rate of antobody response, but normal breathing seemed to be the best.
No remarkable differences were observed between the group inhaled for 10 seconds and for 60 seconds, but severe reactions such as fever were recognized more frequent in the group inhaled for 60 seconds. No irregularity in antibody response was observed by 10 second inhalation method.
Experiments in white mice show that only 3 seconds of inhalation time is enough to provoke regular antibody response and even one second resulted in no marked irregularity in antibody response.
Inhalation time and ability to make antibody response was lineally proportionate in very wide range at least from 3 seconds to 50 minutes.

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Eight strains of influenza virus and one strain of HVJ (Parainfluenza 1 virus) were tested for pathogenicity and immunogenicity for human volunteers. only one strain of influenza B virus was pathogenic and all other strains were not pathogenic and they preserved immunogenicity even after many egg passages.
Influenza A2, the Okuda and the Kinugasa strains, which were pathogenic in the 3rd and the 7th egg Passages, did not show pathogenicity in the 22nd and the 17th generations and they preserved immunogenicity in the same degree after 269 and 231 egg passages respectively.

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