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Volume 15, Issue 3
Displaying 1-5 of 5 articles from this issue
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Akiko OKADA1965 Volume 15 Issue 3 Pages 55-64
Published: June 30, 1965
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSA Viral growth enhancing factor (enhancer) was produced in the chorioallantoic membranes of eggs infected with influenza virus (PR8) and was released into the allantoic fluids. Enhancer was detected by its activity to enhance HA production by HVJ in roller tube cultures of shell-membrane pieces. The enhancement of HA production was obtained either by preincubation of the cultures with enhancer at 37°C for 24hours before challenge of HVJ, or by its addition into the cultures simultaneously with challenge.
The production and release of enhancer occurred in the early stages of infection with PR8 virus, Enhancer activity of the allantoic fluid and chorioallantoic membranes declined rapidly after 24hours of infection and was followed by the appearance of interferon activity. The time when enhancer activity was increasing and maintained a high level corresponds with the time when virus was growing approximately at a linear slope. Interferon activity first appeared in the chorioallantoic membranes and allantoic fluids at 36hours after infection with PR8 virus, when virus growth had reached a maximal level. Subsequent rise in interferon titers was associated with decline of cellular virus titers.
Interferon blocked enhancer activity when added to cultures of shell-membrane pieces before or after the enhancer, as well as when added at the same time as enhancer. Alternatively, enhancer blocked to some extent interferon activity when added before or after the interferon, as well as when added at the same time as interferon.View full abstractDownload PDF (1418K) -
Jutaro TAWARA, Jun SHIBATA, Kouzou FUJITA, Sakae MURAKAMI1965 Volume 15 Issue 3 Pages 65-74
Published: June 30, 1965
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSComparative observations were made by electron microscopy and light microscopy with adjoining sections prepared serially from the same HeLa cell infected with adenovirus type 12. Eosinophilic granules uniformly distributed in the nucleus at the initial stage of virus infection proved to be condensed masses of karyoplasm and subsequently tne peripheral region of the nucleus became more transparent with a deep purplish red inclusion body in the center. The inclusion body was shown to be a conglomeration of eosinophilic structureless granules, karyoplasm and crystalline arranged virus particles. The inclusions gradually became condensed as infection advanced, and finally the entire nucleus became transparent, containing a few electron dense structureless eosinophilic granules with smooth margin, sparsely scattered. Since these granules increased in number and size along with the increase in the number of virus particles, they are regarded as metabolic products accompanying virus replication. The densely stained inclusion body in the center of nucleus contained groups of crystalline arranged virus particles and the transparent portion was replete with numerous virus particles in noncrystalline arrangement. In either arrangement, the virion measured 65-70mμ in diameter, consisting of an electron dense core and a limiting membrane, which corresponded capsid shown by negative staining. The virion is confirmed to be of icosahedron.View full abstractDownload PDF (6103K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...1965 Volume 15 Issue 3 Pages 75-88
Published: June 30, 1965
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSDownload PDF (2158K) -
[in Japanese]1965 Volume 15 Issue 3 Pages 89
Published: June 30, 1965
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSDownload PDF (124K) -
[in Japanese], [in Japanese]1965 Volume 15 Issue 3 Pages 89a-92
Published: June 30, 1965
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSDownload PDF (558K)
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