Uirusu
Online ISSN : 1884-3433
Print ISSN : 0042-6857
ISSN-L : 0042-6857
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Volume 31, Issue 2
Displaying 1-7 of 7 articles from this issue
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  • Clinic and Epidemiology of Human Cytomegalovirus (Review)
    Shunzo CHIBA
    1981 Volume 31 Issue 2 Pages 111-121
    Published: December 01, 1981
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.31.111
    JOURNAL FREE ACCESS
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  • Tumorgenesis due to onco virus
    special reference to avian sarcoma viruses (Review)
    Kumao TOYOSHIMA
    1981 Volume 31 Issue 2 Pages 123-144
    Published: December 01, 1981
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.31.123
    JOURNAL FREE ACCESS
    Download PDF (5847K)
  • Production of Antibodies against Measles Virus and HBs Antigen by Use of the Mouse Hybridoma Technique
    Takehiro TOGASHI, Shigeru KUWAJIMA, Takeyasu TAKEBAYASHI, Hiroyoshi IT ...
    1981 Volume 31 Issue 2 Pages 145-152
    Published: December 01, 1981
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.31.145
    JOURNAL FREE ACCESS
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    Several mouse hybridoma cell lines producing antibodies against measles virus and HBs antigen were made by fusion of spleen cells, which were obtained from BALB/c mice immunized with these antigens, with P3X63Ag8 myeloma cells. The production of antibodies was screened by a radioimmunoassay using antigenprecoated polystyrene balls as a solid phase.
    Twenty three hybridomas producing anti-measles antibody and 5 anti-HBs antibody were obtained as ascites form after intraperitoneal inoculation of hybridomas.
    Out of 23 hybridomas producing antibodies against measles virus which showed very high but different values between HI and ELI antibody titers, 10 were producing antibodies against 79k measles hemagglutinin polypeptide. Cell membrane and cytoplasma of human B cell line persistently infected with measles virus (NC-S) showed a specific fluorescence when stained by hybridoma ascites and FITC labelled anti-mouse sera.
    Hybridoma ascites producing anti-HBs antibody showed PHA titers as high as 400-320, 000fold. By Sephadex G-200 filtration and Ouchterlony technique it was found that antibodies from 4 hybridomas belonged to IgG class and those from one to IgM class. Subtype of HBs antigen was determined by R-PHA using sera of which subtypes of HBs antigen were already known and sheep erythrocytes coated by 3 hybridoma ascites, 2 of which were anti-r and one was anti-common determinant(s) including subtype a.
    The present study suggests that these specific antibodies made by hybridoma technique will be useful for determining the localization of both measles virus and HBs antigen on the pathological materials obtained by biopsy and autopsy.
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  • Isolation of Human Rotavirus in Cell Cultures and its Antigenic and Physicochemical Properties
    Kunihiko SATO
    1981 Volume 31 Issue 2 Pages 153-163
    Published: December 01, 1981
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.31.153
    JOURNAL FREE ACCESS
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    Five cytopathic rotavirus strains were isolated in MA104 cells from fecal specimens of pediatric patients with acute gastroenteritis. In these experiments the virus was treated with trypsin before inoculation and inoculated cultures were rotated in maintenance medium containing a small amount of trypsin. In neutralization tests on acute and convalescent sera from patients with acute gastroenteritis significant titer rises were shown with all the isolates. Rotavirus particles were shown by electron microscopy in cultures infected with the isolates. The isolates showed a close antigenic relationship with the Wa strain of human rotavirus and some antigenic relationship with the Lincoln strain of bovine rotavirus. The isolates were shown to be RNA virus, because their replication was inhibited with 5-iodo-2′-deoxyuridine. The infectivity of the isolates was stable at pH3, resistant to chloroform, ether and sodium deoxycholate, relatively heat-stable and were not stabilized in the presence of 1-M MgCl2.
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  • A new method to stabilize erythrocytes for hemagglutination and hemagglutination inhibition test
    Shosaku MOTODA, Seiya SATO, Isao IWASE, Hiroshi OCHIAI, Seihachiro NIW ...
    1981 Volume 31 Issue 2 Pages 165-166
    Published: December 01, 1981
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.31.165
    JOURNAL FREE ACCESS
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  • Variation of diarrhea viruses detected in Japan in special reference to spherical viruses other than rotaviruses
    Nakao ISHIDA, Tasuke KONNO, Shozo URASAWA, Toru NAKAO, Shigeo MATSUNO, ...
    1981 Volume 31 Issue 2 Pages 167-170
    Published: December 01, 1981
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.31.167
    JOURNAL FREE ACCESS
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  • Round Table Discussion on Influenza Virus Vaccines
    1981 Volume 31 Issue 2 Pages 171-178
    Published: December 01, 1981
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.31.171
    JOURNAL FREE ACCESS
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