Uirusu
Online ISSN : 1884-3433
Print ISSN : 0042-6857
ISSN-L : 0042-6857
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Volume 18, Issue 1
Displaying 1-8 of 8 articles from this issue
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  • COMPARISON OF BASE COMPOSITIONS OF RNA FROM VARIOUS RNA PHAGES
    Satoshi OSAWA, Tohru NISHIHARA, Itaru WATANABE
    1968 Volume 18 Issue 1 Pages 1-5
    Published: 1968
    Released on J-STAGE: November 29, 2010
    DOIhttps://doi.org/10.2222/jsv.18.1
    JOURNAL FREE ACCESS
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    Six coliphages containing RNA, MS2, MY, GA, SD, Qβ and VK, were purified and base ratios of their RNA were determined by paper chromatography. RNA from these RNA phages contained uracil in mole percent, 24.1, 24.6, 27.8, 28.1, 29.3, and 29.3 for MS2, MY, GA, SD, Qβ and VK, respectively. The ratios, A/U and A+C/G+U were 0.98, 1.00 for MS2; 0.95, 0.97 for MY; 0.86, 0.94 for GA; 0.84, 0.92 for SD; 0.79, 0.87 for Qβ, and 0.78, 0.88 for VK.
    From these results, it was shown that these six RNA phages were separated in three distinct groups (MS2, MY: GA, SD: Qβ, VK). These results were in good accordance with groupings by a serological method and a Millipore filtration method. These facts suggest that RNA from the phages of these three groups were characteristic to each group.
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  • ANTIGENIC ANALYSIS OF JAPANESE ENCEPHALITIS VIRUS BY CROSS NEUTRALIZATION TEST
    Chikara AIZAWA, Isao YOSHIOKA, Hiroo YAMAGISHI, Shiro KASAHARA
    1968 Volume 18 Issue 1 Pages 6-10
    Published: 1968
    Released on J-STAGE: November 29, 2010
    DOIhttps://doi.org/10.2222/jsv.18.6
    JOURNAL FREE ACCESS
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    Ten strains of Japanese encephalitis virus isolated in Japan, Taiwan and Thailand, were investigated by plaque reduction neutralization method.
    Cross neutralization tests with rabbit earlyimmune sera demonstrated that all the viruses are divided into three groups. However, those three groups are not independent, but relativeclosely related each other.
    Nakayama-NIH strain is closely related to Nakayama-Yakken and Warren strains which are sublines of Nakayama-NIH strain, but not related to other viruses.
    Geographic variation in the antigenic character of Japanese encephalitis viruses was not observed by our cross neutralization tests.
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  • HARUNA VIRUS, A GROUP A ARBOVIRUS ISOLATED FROM SWINE IN JAPAN
    Tatsuo MATSUYAMA, Tadayoshi NAKAMURA, Keiko ISAHAI, Akira OYA, Masayos ...
    1968 Volume 18 Issue 1 Pages 11-14
    Published: 1968
    Released on J-STAGE: November 29, 2010
    DOIhttps://doi.org/10.2222/jsv.18.11
    JOURNAL FREE ACCESS
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    During June and September, 1965, 14 agents were recovered from blood samples of swine collected in Gumma Prefecture.
    Thirteen of the isolates were Japanese encephalitis virus, and one strain (JaGAn 18565) was identified as group A arbovirus.
    This virus was referred to as Haruna virus because it was isolated from swine at Haruna area.
    Haruna virus shared a common antigen with group A arbovirus and it was closely related antigenically to Getah virus recovered from mosquitoes in Malaya.
    Sucrose-acetone extraction of infected suckling mouse brains with Haruna virus yielded hemagglutinins and antigens additionally treated with protamine sulfate were more potent than untreated antigens. Haruna virus produced the most potent hemagglutinin than any Getah complex viruses.
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  • A CONVENIENT AND EXPLICIT METHOD FOR HEMAGGLUTINATION AND HEMAGGLUTINATION-INHIBITION REACTION OF RUBELLA VIRUS
    Morihiro MORITA, Tsunehisa SUTO, Tomiko TSUTAYA, Yorio HINUMA, Nakao I ...
    1968 Volume 18 Issue 1 Pages 15-22
    Published: 1968
    Released on J-STAGE: November 29, 2010
    DOIhttps://doi.org/10.2222/jsv.18.15
    JOURNAL FREE ACCESS
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    The previous procedures of hemagglutination (HA) and hemagglutination inhibition (HAI) reactions with rubella virus was evaluated and an improved method giving more explicit results was established.
    Baylor strain of rubella virus (MOI=about 1/2000) was inoculated in BHK-21 cell monolayer cultures with Eagle's minimum essential medium in Hanks' BSS without serum and incubated at 33°C using a roller drum.
    High titer (32 to 128) of HA in the culture fluid was obtained continuously from 4 days after the infection for 6 days when the medium was exchanged every day. The harvested fluid was treated with Tween 80 and ethyl ether and resulted 2 to 8 folds increase of HA titer.
    The improved procedures for HA test using Microtiter was as follows: The diluent for every factor was a phosphate buffered saline of pH 6.6 suplemented with 0.1% bovine plasma albumin and 0.2% goose erythrocyte suspension was used. Incubation was performed at 4°C for at least for 90 minutes. For HAI test, the sera to be examined were pretreated with 25% acid-washed kaolin and then absorbed with goose erythrocytes.
    By the procedure, the convalescent sera from rubella patients usually showed the HAI antibody titer of 512 or more.
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  • [title in Japanese]
    1968 Volume 18 Issue 1 Pages 23-40
    Published: 1968
    Released on J-STAGE: November 29, 2010
    DOIhttps://doi.org/10.2222/jsv.18.23
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1968 Volume 18 Issue 1 Pages 41-53
    Published: 1968
    Released on J-STAGE: November 29, 2010
    DOIhttps://doi.org/10.2222/jsv.18.41
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1968 Volume 18 Issue 1 Pages 53-72
    Published: 1968
    Released on J-STAGE: November 29, 2010
    DOIhttps://doi.org/10.2222/jsv.18.53
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1968 Volume 18 Issue 1 Pages 73-96
    Published: 1968
    Released on J-STAGE: November 29, 2010
    DOIhttps://doi.org/10.2222/jsv.18.73
    JOURNAL FREE ACCESS
    Download PDF (3831K)
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