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Volume 30, Issue 2
Displaying 1-4 of 4 articles from this issue
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Takashi Kitamura1980 Volume 30 Issue 2 Pages 87-97
Published: December 01, 1980
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDownload PDF (1715K) -
Yoshimi Kawade1980 Volume 30 Issue 2 Pages 99-109
Published: December 01, 1980
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDownload PDF (1803K) -
Toru SHIMOMURA, Yuko OHASHI, Nakao ISHIDA1980 Volume 30 Issue 2 Pages 111-118
Published: December 01, 1980
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSRoss (1961) reported the acquired resistance to virus infection in plants, which was thought to be similar to the antiviral activity induced by interferon in animal cells. In this study we aimed to know the mechanism of the acquired resistance in plants by using 9-methylstreptimidone (9-MS) which specifically inhibits the virus multiplication in infected animals.
This antibiotic greatly inhibited the number of local lesions produced on leaves of ‘Samsun NN’ tobacco inoculated with tobacco mosaic virus (TMV). The size of the local lesions significantly decreased in leaves treated with 9-MS. The inhibitory effect of 9-MS was shown even when TMV was inoculated on the lower surface of leaves and then 9-MS was rubbed on the upper surface. This suggests a possibility that 9-MS may penetrate into leaf tissues. By the leaf disc method, the effect of 9-MS on TMV multiplication was confirmed in ‘Samsun’ and ‘Bright Yellow’ tobaccos, both of which were systemic hosts of TMV. No remarkable inhibition of lesion formation was observed in leaves inoculated with the mixture of TMV and 9-MS, suggesting no direct effect of 9-MS on TMV. In addition, 9-MS was found to inhibit the synthesis of nucleic acid and protein in the TMV fraction prepared from TMV-infected leaves, but that in ribosomal fraction was not so much inhibited under the same condition. High temperature (30°C) or actinomycin D, which blocks the induction of acquired resistance in plants, did not affect the 9-MS induced resistance.
Similar inhibitory effect of 9-MS was also observed in cowpea leaves inoculated with cucumber mosaic virus.View full abstractDownload PDF (2581K) -
Haeng Duk HAN, Tetsuo KATOH1980 Volume 30 Issue 2 Pages 119-124
Published: December 01, 1980
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSA microplaque assay for morbilliviruses was developed by using carboxymethyl cellulose overlay and Vero cells, Microplaques formed by the strains of measles virus used in this experiment were distinct and easily countable with the naked eye. There was a linear relationship between the numbers of microplaques and virus concentrations. Plaque titers determined by this assay were almost the same as those obtained by the conventional macromethod. The microplaque titrations performed on measles virus were highly reproducible. The microplaque assay was applied to neutralization tests by means of the serum-dilution using a set of microdiluter and microtransfer plate. Neutralizing antibody titers against measles virus determined by this test were four times higher than those measured by the macro-CPE method and corresponded to those by the macro-plaque method. The diameters of microplaques formed by rinderpest and canine distemper viruses were 0.1 to 0.5mm and 0.1 to 0.3mm, respectively. The micro-method for the plaque titration of both viruses was shown to be less sensitive than the macro-method. It was suggested, however, that the sensitivity of the method for these viruses might be increased by improving the overlay medium.
The present technique would be applicable to other viruses by a little modification of composition of media.View full abstractDownload PDF (1912K)
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