Uirusu
Online ISSN : 1884-3433
Print ISSN : 0042-6857
ISSN-L : 0042-6857
Volume 35, Issue 1
Displaying 1-7 of 7 articles from this issue
  • I. In vivo studies
    Masao YAMADA, Shiro NII
    1985 Volume 35 Issue 1 Pages 1-10
    Published: June 01, 1985
    Released on J-STAGE: March 16, 2010
    JOURNAL FREE ACCESS
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  • II. In vitro studies on virus-cell interactions
    Masao YAMADA, Shiro NII
    1985 Volume 35 Issue 1 Pages 11-15
    Published: June 01, 1985
    Released on J-STAGE: March 16, 2010
    JOURNAL FREE ACCESS
    Download PDF (664K)
  • Koki TANIGUCHI, Shozo URASAWA
    1985 Volume 35 Issue 1 Pages 17-34
    Published: June 01, 1985
    Released on J-STAGE: March 16, 2010
    JOURNAL FREE ACCESS
    Download PDF (4172K)
  • An Analysis Based on Publications
    M. Hamaguchi, Y. Iwakura, Y. Fukumi, A. Ishihama
    1985 Volume 35 Issue 1 Pages 35-46
    Published: June 01, 1985
    Released on J-STAGE: March 16, 2010
    JOURNAL FREE ACCESS
    Download PDF (1054K)
  • Yoshiya YOSHIDA, Yumiko FURUYA, Masayasu INOUE, Akiyoshi MATSUSHIMA, K ...
    1985 Volume 35 Issue 1 Pages 47-51
    Published: June 01, 1985
    Released on J-STAGE: March 16, 2010
    JOURNAL FREE ACCESS
    Several monoclonal antibodies were produced against purified HBs antigens, subtype adr adw and ayw. The subtype specificity of these nine antibodies was characterized by the passive hemagglutination inhibition method. The specificity of these anti-HBs monoclonal antibodies was analyzed with use of 31 HBs antigen panels. Five of the nine monoclonal antibodies had a specificity against the group a-determinant of HBs antigen. Two antibodies were demonstrated to have anti-r specificity and another two were anti-w.
    One of these anti-a monoclonal antibodies, in its reaction pattern, resembled anti-a polyclonal antibady derived from human serum. This anti-a monoclonal antibody was used as the standard to determine HBs antigen subtyping, The results nearly agree perfectly with results obtained when anti-a polyclonal antibodies were used as the standard.
    Using these monoclones, we analysed HBs antigen subtypes of asymptomatic carriers. Although the antigen titer changed at various time in each parson, the ratio of each subtype determinant was unchanged. However, three anti-a monoclonal antibodies showed a different reaction pattern against “a” determinant of individual carriers, and therefore “a” determinant of HBs antigen might be subdivided, and should be analyzed in more detail by monoclonal antibodies.
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  • Keiko SHIMOKAWA, Tsugiya MURAYAMA, Toru FURUKAWA
    1985 Volume 35 Issue 1 Pages 53-61
    Published: June 01, 1985
    Released on J-STAGE: March 16, 2010
    JOURNAL FREE ACCESS
    Replication of human cytomegalovirus (HCMV) was inhibited by 0.2μg/ml of tunicamycin (TM), which blocks the synthesis of glycoproteins. In the presence of TM, HCMV infected cells failed to permit viral DNA replication, expression of viral late antigens and formation of viral particles. Nevertheless, early cell rounding and the development of early immuno-fluorescent antigens were expressed in the presence of TM. Removal of TM resulted in restoration of virus titer and DNA synthesis within 72h. SDS-PAGE analysis revealed that TM-treated cells synthesized most early proteins at levels comparable to levels of synthesis in untreated cells.
    The results suggest that TM blocks mainly late stage of the replicative cycle, and it may related with failing to synthesis a glycoprotein (s) appeared in the early phase of infection.
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  • 1985 Volume 35 Issue 1 Pages 63-64
    Published: June 01, 1985
    Released on J-STAGE: March 16, 2010
    JOURNAL FREE ACCESS
    Download PDF (191K)
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