Uirusu
Online ISSN : 1884-3433
Print ISSN : 0042-6857
ISSN-L : 0042-6857
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Volume 21, Issue 2
Displaying 1-5 of 5 articles from this issue
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  • STUDIES ON COMPLEMENT FIXATION TEST OF AVIAN SERUM
    Akiko FUKUDA
    1971 Volume 21 Issue 2 Pages 41-49
    Published: July 01, 1971
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.21.41
    JOURNAL FREE ACCESS
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    Various test conditions were examined to establish the complement fixation (CF) test procedures of chicken sera for practical application. From the experiment in which fresh duck, goose, and pigeon sera were tested for their cofactor (avian C′1) activity that is essential for CF reaction of avian antibody, duck serum was proved to be most suitable as cofactor. Thus, standard CF procedure was established by employing a given amount of fresh duck serum in the reaction system, and was shown to have higher reproducibility and to be more practical than the other methods previously reported. The procedure was successfully applied for detection of antibody to Newcastle disease virus in convalescent and hyperimmune chicken sera, and antibody to Mycoplasama galisepticum in convalescent chicken sera.
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  • SOME PHENOMENA OBSERVED IN SYSTEMIC INFECTION OF ALFALFA MOSAIC VIRUS
    I. Factors concerned with necrotic symptom development
    Tadao MISAWA, Akira KUDO
    1971 Volume 21 Issue 2 Pages 50-54
    Published: July 01, 1971
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.21.50
    JOURNAL FREE ACCESS
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    Necrotic symptom occurs on broad bean by local and systemic infection caused by alfalfa mosaic virus. Some factors concerning the occurence of this necrotic symptom were observed.
    The results are presented in this paper.
    1) Occurence of necrotic symptom of inoculated leaf is closely associated with the degree of multiplication of the virus. In an inoculated young leaf which the virus increases more rapidly than in an old lower leaf, necrotic symptom appears more sever.
    2) Wherever inoculation is done on any part (leaf, leaf-petiole and stem), if the virus multiplies sufficiently there, necrosis develops always almost concurrently on systemically infected apical leaves. When lower leaves and lower part of stem were inoculated, necrosis of apical leaf is difficult to appear.
    3) The virus concentration in the inoculum influences remarkably the development of necrosis on apical leaves. When dense inoculum was used, the virus multiplies much more in inoculated leaf and necrosis of apical leaf appears in higher rate.
    4) Appearence of necrosis of apical leaf relates with the growth stage of upper leaves. Leaves which exhibit necrosis due to systemic infection are limited to unexpanded apical leaves smaller than about 24mm in length at the time of inoculation.
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  • A MICROTITER METHOD FOR ASSAY OF NEUTRALIZING ANTIBODIES AGAINST GROUP B ARBOVIRUSES
    Nobuo HASHIMOTO, Kenichiro YAMADA, Masatsugu KANAMITSU
    1971 Volume 21 Issue 2 Pages 55-59
    Published: July 01, 1971
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.21.55
    JOURNAL FREE ACCESS
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  • SMON (Subacute Myelooptico-neuropathy)
    [in Japanese]
    1971 Volume 21 Issue 2 Pages 60-61
    Published: July 01, 1971
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.21.60
    JOURNAL FREE ACCESS
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  • Abstracts of the 8th Annual Meeting of the Kyushu Branch, the Society of the Japanese Virologist
    1971 Volume 21 Issue 2 Pages 62-71
    Published: July 01, 1971
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.21.62
    JOURNAL FREE ACCESS
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