Uirusu
Online ISSN : 1884-3433
Print ISSN : 0042-6857
ISSN-L : 0042-6857
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Volume 24, Issue 4
Displaying 1-5 of 5 articles from this issue
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  • APHID TRANSMISSION OF PLANT VIRUSES
    Tadao MISAWA
    1974 Volume 24 Issue 4 Pages 291-299
    Published: December 31, 1974
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.24.291
    JOURNAL FREE ACCESS
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  • STUDIES ON HEMAGGLUTINATION AND HEMAGGLUTINATION INHIBITION REACTION OF ECHO-6 ISOLATES PASSED THROUGH HUMAN EMBRYONIC LUNG OR KIDNEY CELL CULTURES
    Muneyuki MATSUDA, Koji KATORI, Mitsue MIYAHARA, Toshiko HASHIZUME, Ken ...
    1974 Volume 24 Issue 4 Pages 300-308
    Published: December 31, 1974
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.24.300
    JOURNAL FREE ACCESS
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    The hemagglutination (HA) of ECHO-6 virus and the utilization of hemagglutination-inhibition (HI) for diagnosis were investigated with virus isolates and sera from some patients involved in an epidemic of infection with this virus in Toyama in 1965.
    1. MK-passed in virus showed a maximum hemagglutination and cytopathic effect (CPE) 5 or 6 days, and infectivity 2 days after inoculation, while HEL- or HEK-passed virus showed a maximum hemagglutination and infectivity only 1 or 2 days and CPE 3 days after inoculation.
    2. The loss of hemagglutinating activity and, in general, quite a distinct variation in HA titer were observed among such strains as passed through MK cell cultures. Only 28 strains (58.33%) out of 48 showed positive HA after 3 passages in MK cell culture. In contrast, no loss of HA was observed among strains passed through HEL or HEK cell cultures. The rate of HA-positive strains increased gradually with the advance in passage. Finally only 2 out of the 48 strains were negative for HA after 3 passages in HEL or HEK cell culture.
    3. The mean HA titer after 3 passages in MK cell culture was only 3 times that of the primary culture, whereas that after 3 passages in HEL or HEK cell culture was 15 times.
    4. Fifteen HA-positive and 3 HA-negative strains were identified as ECHO-6 virus by the hemagglutination-inhibition and the neutralization and the neutralization test, respectively.
    5. The hemagglutination-inhibition test with patient's sera was a useful method for diagnosis in the early stage, since relatively high HI titers were observed in convalescent sera.
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  • ELECTRON-DENSE PARTICLES APPEARED IN PERIPHERAL HUMAN LEUKEMIC CELLS TREATED WITH 5-BROMODEOXYURIDINE
    Yoh TANAMI, Yoshitsugu YAMADA
    1974 Volume 24 Issue 4 Pages 309-320
    Published: December 31, 1974
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.24.309
    JOURNAL FREE ACCESS
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    In an electron microscope study, it was demonstrated that particles resembling the type C particles had been produced in peripheral leukemic cells or leukocytes obtained from five patients with leukemia and one with reticulum cell sarcoma when these cells had been exposed to 5-bromodeoxyuridine in a short-termed culture. They had electron-dense sphere cores surrounded by a single nuit membrane and varying from 60 to 100nm in outer diameter. Fairly frequently, they appeared in clusters in intracytoplasmic vesicles formed in leukemic myelogenous cells or leukocytes. They were found neither in untreated leukocytes nor in drug-treated lymphocytes or eosinocytes obtained from the patients.
    Similar electron-dense particles were also demonstrated in peripheral myelogenous leukocytes obtained from three of 10 healthy persons examined after exposure to the drug under the same experimental conditions as mentioned above. No biological activity of these particles was clarified.
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  • [title in Japanese]
    1974 Volume 24 Issue 4 Pages 321-326
    Published: December 31, 1974
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.24.321
    JOURNAL FREE ACCESS
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  • Virus cell interaction
    1974 Volume 24 Issue 4 Pages 327-390
    Published: December 31, 1974
    Released on J-STAGE: March 16, 2010
    DOIhttps://doi.org/10.2222/jsv.24.327
    JOURNAL FREE ACCESS
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