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Volume 24, Issue 2
Displaying 1-7 of 7 articles from this issue
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Katsuro KAWAKAMI1974 Volume 24 Issue 2 Pages 145-156
Published: August 01, 1974
Released on J-STAGE: March 16, 2010
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Masao UMETSU, Shunzo CHIBA, Setsuko OGAWA, Tooru NAKAO1974 Volume 24 Issue 2 Pages 157-163
Published: August 01, 1974
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSIt was reported previously that serum antibodies against Mycoplasma pneumoniae mainly belonged to IgG and IgM and that these immunoglobulins appeared subsequently to infection with this organism. The ratio of IgG to IgM antibodies showed a considerable individual variation even when compared at approximately the same time after the onset of illness. There was no definite explanation for this individual variation.
The present study was carried out on the serum immunoglobulin response of children with M. pneumoniae infection, especially the chronological change of specific IgG and IgM titers measured by the indirect immunofluorescent (IMF) technique. The chronological change of IgG and IgM IMF titers was compared with that of complement-fixing (CF) and cold hemagglutination (CHA) antibody titers. Many patients showed an apparent subsequent appearance of IgG IMF antibody, and this appearance was similar to that of CF antibody. An individual variation was observed in specific IgG IMF antibody response and in the persistence of this antibody.
There were some patients who had no detectable IgM IMF antibody. All of them exhibited a significant rise in CF and IgG IMF titers. These results agreed with those obtained from some cases which responded to M. pneumoniae infection with the production of antibodies of IgG class almost exclusively. All those patients presented a significant change in titer of CHA antibody which belonged to the IgM class. The results agreed with those reported by previous authors that some patients showed an increase of nonspecific serum IgM level during infection with M. pneumoniae, whereas they had specific antibodies belonging almost exclusively to the IgG class. The patients studied in the present investigation included some who possessed preexisting specific antibody. An individual variation in IgM IMF antibody response was also proved in these patients. It was considered that the experience of reinfection with M. pneumoniae might have no important effect upon the individual variation, and that after the secondary immune response to M. pneumoniae infection IgM antibody might be detectable in some patients.View full abstractDownload PDF (948K) -
Yoh TANAMI, Yoshitsugu YAMADA1974 Volume 24 Issue 2 Pages 164-169
Published: August 01, 1974
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSIn an electron microscopic study it was demonstrated that type C particles (L cell virions, LCV) were produced at a relatively high frequency in Earle's L cells (929 subline) when these cells had been incubated in the presence of 5-bromodeoxyuridine (BuDR) at a concentration of 10-4M. LCV appeared within the intracytoplasmic vesicles of L cells at times later than 20 hours after the initiation of BuDR treatment. LCV particles were produced from the limiting membrane of the vesicles by the budding process toward the inside of the vesicles. Each of them had an electron-dense, coiled core 128±31nm in outside diameter coated by a single unit membrane (envelope). Such knobs as noted by some previous authors were not recognized on their surface.View full abstractDownload PDF (9518K) -
Shinji KAJITA, Chiaki MATSUI1974 Volume 24 Issue 2 Pages 170-174
Published: August 01, 1974
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSModifications of tobacco mosaic virus and its RNA caused by treatment with sodium bisulfite were examined. After this treatment, of all the four basic components of naked viral RNA, cytidine residue decreased by about 9.6% and uridine residue increased by about 9.8% but guanosine and adenosine residues underwent no changes at all. On the other hand, no changes occurred to any basic component of RNA extracted from treated virus particles. In a sucrose density gradient made with 0.02M tris-HCl buffer, sedimentation velocity was slower in sodium bisulfite-treated viral RNA than in untreated viral RNA. In a sucrose density gradient made with tris-HCl buffer containing 0.1M NaCl, however, the sedimentation velocity of the treated RNA was the same as that of the untreated RNA. The sodium bisulfite-treated viral RNA decreased rapidly in infectivity. Although the infectivity of the virus particles did not change after sodium bisulfite treatment for 30 minutes, it decreased by about 30% after the treatment for 2 to 48 hours.View full abstractDownload PDF (656K) -
Kenkichi SHINKAI, Takuzo NISHIMURA1974 Volume 24 Issue 2 Pages 175-180
Published: August 01, 1974
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSStudies were carried out to demonstrate some difference in antiinfluenza virus activity between Virazole (Vz) and adamantadine hydrochloride (Ad) by means of the quantitative hemadsorption (QHA) technique. The results obtained are summarized as follows.
(1) Vz completely suppressed the growth of the B/Lee strain of influenza virus. It reduced the growth of five strains of the type A viruses by 45-80% at a concentration of 33μg/ml. On the other hand, Ad markedly inhibited the growth of the A/AA 2/60 (H2N2), A/Japan 305/57 (H2N2), and A/Hong kong (H3N2) strains, but had little inhibitory effect on the multiplication of any other strain of the influenza viruses.
(2) When the drugs were added to viral cultures as late as 60 minutes after infection with the A/AA/2/60 (H2N2) strain, Vz reduced the hemadsorption activity of infected cells by 77%, while 10μg/ml of Ad exerted no significant inhibition upon viral growth. Neither drug, however, affected the adsorption of the influenza A strain onto chick embryo cells.View full abstractDownload PDF (719K) -
Kenkichi SHINKAI1974 Volume 24 Issue 2 Pages 181-183
Published: August 01, 1974
Released on J-STAGE: March 16, 2010
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1974 Volume 24 Issue 2 Pages 184-191
Published: August 01, 1974
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSDownload PDF (1162K)
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