Uirusu

Host immune response to herpes simplex virus infections

K. Hayashi

1989 Volume 39 Issue 1 Pages 1-19
Published: June 01, 1989
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.2222/jsv.39.1

JOURNAL FREE ACCESS

Download PDF (3021K)
Antibody-dependent viral infection in macrophages

Hiroshi OCHIAI, Masahiko KUROKAWA

1989 Volume 39 Issue 1 Pages 21-28
Published: June 01, 1989
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.2222/jsv.39.21

JOURNAL FREE ACCESS

Download PDF (1147K)
Antigenic structures of human parainfluenza viruses

Y. Ito, M. Tsurudome, H. Komada, H. Tomoto

1989 Volume 39 Issue 1 Pages 29-45
Published: June 01, 1989
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.2222/jsv.39.29

JOURNAL FREE ACCESS

Download PDF (8793K)
Changes in cerebral level of monoamines by Japanese encephalitis virus infection

Yoshiaki Yamashita, Tomiyoshi Ito, Iwao Hashimoto, Akio Ohyama, Kinya ...

1989 Volume 39 Issue 1 Pages 47-54
Published: June 01, 1989
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.2222/jsv.39.47

JOURNAL FREE ACCESS

Show abstractHide abstract

The changes in monoamine levels of different brain regions following Japanese encephalitis virus (JEV) intraperitoneal inoculation were examined in experimentally JEV-infected mice. In addition, virus distribution was studied using infectivity assay and immuno-histochemistry of viral antigen.
1) The level of monoamines in brain tissues was not affected by 48 hours after viral inoculation, but marked effects were elicited at 96 hours after the inoculation. The cerebral concentration of 5- hydroxyindole-3-acetic acid (5 HIAA) was increased, while that of dopamine (DA) showed a decrease. Especially these alteration were observed in the cerebral cortex, but not in the cerebellum.
2) The viral growth in the brain was observed at 48 hours after the inoculation. The growth in the cerebellum, however, was found to be lower than those in other cerebral regions.
3) The viral antigen was detected in the cerebral cortex, hippocampus, mesencephalon and diencephalon in addition to the substantia nigra and striatum.
From these results, it is presumed that clinical manifestation of JEV infection may involve the changes in the metabolism of neurotransmitter, especially those of DA and serotonin in the brain.

View full abstract

Download PDF (3190K)
Preparation of monoclonal antibodies against Dusk hepatitis B core antigen and analysis of the manocloual antibodies

T. Saito, K. Tachibana, K. Mogi, H. Mizuo, Y. Ito, M. Imai

1989 Volume 39 Issue 1 Pages 55-60
Published: June 01, 1989
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.2222/jsv.39.55

JOURNAL FREE ACCESS

Show abstractHide abstract

Mice were immunized against duck hepatitis B virus core (DHBc) particles isolated from the liver of asymptomatic carrier ducks of duck hepatitis B virus (DHBV) by ultracentrifugation. Their spleen cells were fused with mouse myeloma (NS-1) cells, and 12 clones of hybridoma cells secreting antibodies against DHBc (anti-DHBc) were isolated. According to the reactivity to core particles and core peptide obtained from DHBc particles treated with SDS-2 ME, the 12 antibodies were classified into two groups. Two monoclonal antibodies reacted against both core particles and core peptide (B-type), the other ten monoclonal antibodies reacted against core particles but did not react against core peptide obtained from DHBc particles treated with SDS-2 ME. (A-type).
Solid phase enzyme immuno assay (EIA) using these two types of antibodies could detect core antigenisity not only in the liver homogenate but also in the DHBV infected serum. Sucrose gradient analysis and gel filtration analysis revealed this DHBc antigenisity in the serum is not carried by core particles but carried by core peptide, equivalent to HBe antigen in the serum of Hepatitis B virus (HBV) carrier.
This EIA may provide sensitive test monitoring both serum DHBe antigen levels and DHBc antigen levels in the liver during DHBV infection.

View full abstract

Download PDF (3259K)
The 22th Summer Symposium of Hokkaido Branch, Society of Japanese Virologists

1989 Volume 39 Issue 1 Pages 61-64
Published: June 01, 1989
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.2222/jsv.39.61

JOURNAL FREE ACCESS

Download PDF (575K)
The 24th Congress Meeting of Hokkaido Branch, Society of Japanese Virologists

1989 Volume 39 Issue 1 Pages 65-74
Published: June 01, 1989
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.2222/jsv.39.65

JOURNAL FREE ACCESS

Download PDF (1355K)
The 26th Congress Meeting of Kyushu Branch, Society of Japanese Virologists

1989 Volume 39 Issue 1 Pages 75-81
Published: June 01, 1989
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.2222/jsv.39.75

JOURNAL FREE ACCESS

Download PDF (964K)
The 24th Meeting of Ecological Study Group of Japanese Encephalitis Virus

1989 Volume 39 Issue 1 Pages 83-95
Published: June 01, 1989
Released on J-STAGE: March 12, 2010

DOIhttps://doi.org/10.2222/jsv.39.83

JOURNAL FREE ACCESS

Download PDF (3323K)

Register with J-STAGE for free!