Basic experiments of ELISA using glass beads as solid phase were thoroughly performed for the application to detect human rota virus from stools. A batch of human rota virus was obtained from a patient stool with infantile gastroenteritis of white colored stool. Antibody was made by immunization of a guinea pig with the highly purified human rota virus. The most important keys to remove nonspecific reaction are the purity of antibody to coat the beads and addition of homologous normal serum to the 2nd phase of ELISA system. Sixty eight cases (26.9%) were positive for human rota virus after application of this system among 253 rectal swabs of diarrheal children obtained at Kawasaki Munincipal Hospital in 1980. On the other hand IAHA method was able to detect the virus in 12% of the same specimens. It is concluded that the ELISA is much available for detection of human rota virus because of short time requirement (4 hours) for the entire procedure and of no necessity of special equipment and finally of its high sensitivity.