The growth rate of Bacillus sp. TSK2 increased when Mg
2+ was externally added to LB medium at 25 ℃ and showed the maximum value, 0.82 (Doubling time h
-1), at 50 mM. While the growth rate was repressed to 0.12 in the presence of 1.0 M NaCl in the medium, the value increased to 0.59 at the same concentration of NaCl, and the growth ability expanded to 3.0 M when 50 mM Mg
2+ was added to the medium. These results indicate that the strain possesses transport system (s) with relatively low affinity to Mg
2+, and a concentration of Mg
2+ similar to that in seawater plays an important role in growth under high NaCl stress.
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