巻号一覧
13 巻 (1984)
- 5 号 p. 237-
- 4 号 p. 183-
- 3 号 p. 121-
- 2 号 p. 59-
- 1 号 p. 1-
前身誌
13 巻, 2 号
選択された号の論文の9件中1～9を表示しています
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宇佐美 英治, 瀬山 義幸, 山下 三郎1984 年 13 巻 2 号 p. 59-65
発行日: 1984/06/25
公開日: 2012/11/27
ジャーナル フリーElastasea ctivity obtained from normal healthy human serum was subjected to CM Sephadex column chromatographic and chromatofocusing separations, and there were two kinds of activities, that is, pancreas and leukocyte originated ones.
Pancreatic elastase activity was suppressed and leukocytic elastase activity was activated by the addition of 0.5M NaCl.The followingfindings were obtained using the discriminating assay method of the pancreatic and leukocytic elastase activities.
The ratio of pancreatic and leukocytic elastase activities in the serum total elastase activity was different in age and physiological Conditions, that is, the serum total elastase (elastin hydrolyzing) activity was low in the patient with hypothyroidism but the ratio of leukocytic elastase was found to be higher.
The ratio of the pancreatic to the leukocytic elastase activities in young (<10 years old) and in old (>70 years old) subjects were examhd, and the ratio of leukocytic dastase activity was fo und to be highr in young subjects.
Elastase activity ih the serum may have different functions, in thelr origins, pancreatic or leukocytic, to regulate metabolic activities in the connective tissues.抄録全体を表示PDF形式でダウンロード (732K) -
, , , , , ,1984 年 13 巻 2 号 p. 66-73
発行日: 1984/06/25
公開日: 2012/11/27
ジャーナル フリーThis paper describes an automated, sensitive method for determination of polyamine in urine.lt is based on luminol-chemiluminescent assaying of hydrogen peroxide generated from polyamine in the presence of putrescine oxidase.
The standard curve obtained with putrescine solutions was linear in the range between 680 pmoles and 20.4 nmoles. The within-assay CV was less than 2%.The results obtained with this method correlated well with those obtained with an enzymatic method based on spectrophotometry of hydrogen peroxide (n=70, y=1.15x-1.77, r=0.994) and with those obtained on high performance liquid chromatography (n=24, y=0.908x+6.41, r=0.995).
The normal range of the polyamine/creatinine ratio in 24 hr urine was less than 34 (μmole/g) for males and less than 50.9 for females. The polyamine/creatinine ratio was elevated remarkably in somepatients with malignant proliferative diseases.抄録全体を表示PDF形式でダウンロード (912K) -
中西 康雄, 五十嵐 富三男, 中山 年正, 北村 元仕1984 年 13 巻 2 号 p. 74-78
発行日: 1984/06/25
公開日: 2012/11/27
ジャーナル フリーThe total calcium values of some post-hemodialysis plasmas measured by two different chelation procedures, the o-CPC colorimetric and the calcein fluorometric titration methods, decrease markedly when samples are stored in a refrigerator.After one day storage, the decrease was found for 9% of all plasmas examined (9 out of 100).Extention of storage time and increases in temperature and pH magnified the decrease.The extent of the decrease (ΔmEq/l) correlated well with the concentration of free fatty acids in the plasma (corrrelative coefficient r=0.81).Treatment of the plasmas whose calcium values had dropped during storage with an inorganic acid (volume ratio of 1: 20 for plasma to 50mmol/l HCl) solved this problem practically.These findings suggest that this phenomenon is due in part to complex formation between calcium and free fatty acids.抄録全体を表示PDF形式でダウンロード (575K) -
Determination of Hemoglobin Using 3-Methyl-2-Benzothiazolinone Hydrazone (MBTH) and Formaldehyde,1984 年 13 巻 2 号 p. 79-84
発行日: 1984/06/25
公開日: 2012/11/27
ジャーナル フリーA sensitive and simple method fon color-producing detenmination of hemoglobin is described. The method is based on oxidative coupling of3-methyl-2-benzothiazolinone hydrazone (MBTH) with formaldehyde-ln the presence of hydnogen penoxide, hemoglobin shows a pseudopenoxidase effect and catalyzes the formation of a deep blue dye as the MBTH for maldehyde coupled, which has a broad absorption between 570 and 700nm with apeak at 635nm. ln the proposed method using the water-methanol (1: 1 by vol.) solvent system, blood components-denived interferences were elimirlated.The method was suited for the measurement of hemoglobin at concentnations of 1-20g/dl.The within-run pnecision (C.V.) was 2.03% with the recovery of 102.6%.A close correlation was found between this and the other methods.抄録全体を表示PDF形式でダウンロード (605K) -
石黒 伊三雄, 太田 好次, 佐々木 恵美, 伊藤 宜則, 篠原 力雄1984 年 13 巻 2 号 p. 85-92
発行日: 1984/06/25
公開日: 2012/11/27
ジャーナル フリーThis study was designed to evaluate changes in the supenoxide dismutase (SOD) quantity in human erythrocytes based on aging and sex differences.
The recovery rate of SOD quantiy in the supernatant after removing hemoglobin with organic solvent (ethylalcohol: CHCl3=5: 3) from whole blood was 96%.SOD activity in whole blood was stable for at least 6 days in refrigerator and SOD activity in the supernatant aften removing hemoglobin was not changed for 5 hours at room temperature.
Both SOD quantity in whole blood of nonmal human subjects and its variations with regand to sex and age were detected.The SOD quantity per 1 ml of whole blood was69.5±15.1μg in males and62.8±11.5μg in females, a significantly lower value in females. However, SOD quantity per hemoglobin (g) was 512.7±104.2μg in males and508.9±93.2μg in females.The SOD quantity per1010RBC was154.9±44.5μg in males and152.6±28.1μg in females.There was a tendency for the SOD quantity in whole blood to decreasewith aging, However, no changes secondary to aging was noted in relation to hemoglobin (g) and erythrocytes.抄録全体を表示PDF形式でダウンロード (889K) -
三輪 一智, 奥田 潤1984 年 13 巻 2 号 p. 93-101
発行日: 1984/06/25
公開日: 2012/11/27
ジャーナル フリーA new method for determining α-amylase isozymes in senum was developed.This method needs the measurement of α-amylase activity in a shgle sample by two different assay methods for the erlzyme.ln one of the two methods, γ-cyclodextrin was used as a substnate.The principle of this differential assay for α-amylase isozymes was based on the fact that γ-cyclodextrin is hydrolyzed by pancreatic α-amylase 3 times mone rapidly than by salivany a-amylase provided that the activities of the two isozymes are of the same value in the other method employing chnomogenic amylose as a substnate.
Analysis of sena from 16 nonmal subjects by the present method showed that pancreatic α-amylase constitutes45.6±10.8% (mean±S.D.) of the total serum α-amylase.This value was in good agreement with those (-50%) reported by others.抄録全体を表示PDF形式でダウンロード (912K) -
武田 立守, 吉田 滋, 織田 佳代子, 広瀬 信吾1984 年 13 巻 2 号 p. 102-108
発行日: 1984/06/25
公開日: 2012/11/27
ジャーナル フリーFifteen kinds of bile acids in serum are determined simultaneously by high-performance liquid chromatography (HPLC) using a new automated pretreatment system.The eluate from separation column (Bile pak; Nihon Bunko) was mixed with NAD and fluorescence intensity of NADH produced by enzymatic reaction with immobilized 3α-hydroxysteroid dehydrogenase was measured.The automated pretreatment system consists of reversedphase column (Serumout-25;Sekisui Chemical Co.), line filter, three ports valve for degas, five ports valve for injector, six ports valve for sampler and pump.With use of this system, most of interfering substances to determine bile acids by HPLC, that is water-soiuble protein, peptide, ion etc, are eliminated with in2-5min.The determination of individual bile acids can be done with 5-10μl of serum without loss or dilution.The recovery rates of cholic acid, glycocholic acid and taurocholic acid added to serum ranged101±2,100±3 and 102±3%, respectively and coefficients of variation ranged from 2.0 to 5.5% (n=5).抄録全体を表示PDF形式でダウンロード (808K) -
, ,1984 年 13 巻 2 号 p. 109-113
発行日: 1984/06/25
公開日: 2012/11/27
ジャーナル フリーA simplified fluorometric microassay of DNA in mononuciear cell preparations using ethidium bromide is described.Optimal assay conditions are as follows;sodium dodecyl Sulfate (0.015%)(2ml) was added to mononuclear cells as a detergent, then ethidium bromide (8μg/ml)(2ml) was added as a fiuorescent intercalating agent.Fluorescence intensity was measured with an excitation at 538nm and an emission at 586nm.Cellular DNA are determined in the range of 1×105-4×106cells.This fluorometric assay is simpie, rapid, highly sensitive, and directly applicable to mononuclear cell preparations.抄録全体を表示PDF形式でダウンロード (546K) -
, , , ,1984 年 13 巻 2 号 p. 114-120
発行日: 1984/06/25
公開日: 2012/11/27
ジャーナル フリーA report is presented on the development of a routine GC/MS assay for urinary steroids screening.Urine is enzymatically hydrolyzed with glusulase, and extracted steroids are converted to their corresponding MO-TMS derivatives and quantitated with a quadropole GC/MS system using SIM (Selected lon Monitoring).Up to thirteen steroid smay be simultaneously quantified.
Data from a few of abnormal urine specimens which 17-Ketosteroids total is presented high or low lelvels are reported and compared to healthy urine specimen.
This method is simple, sensitive and specific and is suitable for following the metabolic profiling of steroids in human urine specimens.抄録全体を表示PDF形式でダウンロード (615K)
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