1) A male patient aged 49 years with pretibial myxedema was examined. He showed hyperthyroidism accompanied with exophthalmus and th roid acropathy. LATS was intensively positive in the blood, but negative in the local tissue. 2) Accumulation of mucopolysaccharides, especially hyaluronic acid, and high content of water were observed in the local tissue. Decrease of chondroitin sulfate B content was also demonstrated. 3) Hyper-activated synthesis of acid mucopolysaccharides in the local tissue was evidenced. Accordingly, it is suggested that the highly activated hyaluronic acid synthesis might result in relative lowering of synthetic activity of chondroitin sulfate B.
Detectable activities of aldolase and of glyceraldehyde phosphate dehydrogenase in human erythrocyte ghost could be varied considerably depending on the conditions of preparing the ghosts which affect adsorption of the enzymes to the membrane or which influence permeability of the membrane to the enzyme substrate. The ghosts prepared in hypotonic veronal buffer contain approximately half of the respective enzyme activity found in intact erythrocytes, which is sometimes not fully measured due to the membrane permeability barrier against the externally-added substrate. These enzyme activities in the membrane, however, could be eluted away by homogenizing the ghost in isotonic saline. The membrane fragments which had been prepared by direct homogenization of intact cells in isotonic saline or by homogenization of ghosts obtained by saponin hemolysis in isotonic medium, contained only a very small part of these enzymes. Therefore, it is suggested that these enzymes are originally not the membrane-bound enzymes but may be the ones artificially adsorbed from cytoplasmic fluid onto inner surface of the plasma membrane when ghosts are prepared under a hypotonic condition.
A one-tube method for measuring both the serum iron concentration and iron-binding capacity is described. Iron is released from transferrin in an acid solution and combined to TPTZ. The color intensity is proportional to the serum iron concentration. An alkaline buffered solution is added to the reaction mixture to make the contents alkaline, dissociate the iron from TPTZ-iron complex, and permit to recombine with transferrin. Subseqently, a known excess of iron is added to the test tube to saturate the iron-binding protein. The surplus unbound iron in solution is determined by complexing it with SBP (Sodium Bathophenanthroline Sulfonic Acid), and this color formed iron-SBP chelate is very stable above pH 9.0. The unsaturated iron-binding capacity of the serum specimens is calculated by the determination of the difference between the quantity of iron added to the test solution and the amount of iron that complexes with SBP.