Analysis of phospholipids e, tracted from the erythrocytes of patients with hereditary spherocytosis revealed that neither the total phospholipid content nor the phospholipid class composition of the membrane differs significantly from what was obtained of normal erythrocytes. The phospholipid class composition of the blood serum of the patients was also normal. A marked decrease in the serum level of β-lipoprotein as well as the lipid components (cholesterol, triglyceride and phospholipid) was noted. The decrease, however, tended to be restored partially after splenectomy. The significance of such hypolipidemia of these patients was discussed.

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As a Technicon's SMA 12/60 (Survey type) Auto Analyzer was introduced to our clinical chemistry laboratory to perform a major role in our routine works, a point was raised particularly from the pediatric department where the amount of serum required (2 ml) was considered to be oo great. In order to solve the problem and improve precision of the re- sults, the following modifications of the SMA 12/60 system have been accomplished.
1. Introduction of predilution system for all channels and ultra violet methods to LDH, GOT and GPT (substituted for glucose channel) using automatic blank correction without a dialyzer, as previously reported by us.
2. Substitution of the bromocresol green method for the HABA method in albumin channel to minimize interference by bilirubin in serum.
3. Removal of a preheating bath from uric acid channel to decrease carry-over contami- nation and addition of a time-delay coil to cholesterol channel to increase the sensitivity of the method.
As a result of the modifications, the sample volume per profile was reduced to 0.4 ml without any sacrifice in speed, accuracy or precision, and the devised system has been successfully performed in our laboratory over 16 months.

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Procedure for the determination of alkaline phosphatase activity in human serum by adaptation of the histochemical method for demonstrating phosphatase activity with alpha naphthyl phosphate as the substrate was described.
The alpha naphthol produced by enzymatic hydrolysis, was converted to a purple azo dye by coupling with Fast blue B salt. Although the azo dye was an insoluble product, it was dissolved with sodium lauryl sulfate solution, and then its optical density was measured colorimetrically.
It may be useful in the diagnosis of liver and bone diseases to determine on serum alkaline phosphatase by this method combined with the zymogram method. The procedure that is adopted are outlined as follows; Determinations are made in duplicates. With a 0.05 ml pipette. serum is transferred into each of two test tubes, which contain 3.0 ml of substrate buffer solution (pH 10.4). Two tubes containing 3.0 ml of the substrate buffer solu- tion without serum are used as a blank.(see to table 1)
These test tubes with or without serum are incubated at 37°C for 30 minutes. 1.0m1 of inhibitory solution is then added to each test tube which is alkalic condition. Then 0.05 ml of serum is added to each tube without serum. And 1.0ml of Fast blue B salt solution is added to all test tubes. The solution is prepared by dissolving 40mg of Fast blue B salt and 5g of sodium lauryl sulfate in 100m1 of citrate buffer (pH 4-8), immediately before use. Each Purple pigment solution obtained from the above reaction, is transferred into a colorimeter tube. Color density is then determined with a photoelectric colorimeter through a 540mμ filter.

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広く用いられている血糖測定用のo-TB試薬中の氷酢酸, H₂O含量を変え, その時の呈色反応の様相を解析した成績を基にして改良o-TB 試薬を調製し, 次の諸成績を収めた。
(1) 高H₂O含有 (35%) o-TB試薬を用い 15分加熱反応することにより反応発色後の呈色の安定化ができた。しかし発色が Plateauに達しない時 (7分) に加熱を中止すると呈色は不安定であった。
(2) この改良試薬を用いて直接法で測定すると, 蛋白質に起因する混濁を生じ高目の血糖測定値が得られた。
(3) しかしin vitroでAlbumin, γ-Globulin を添加しても混濁を生じなかった。
(4) 試薬中に, 或いは反応後にDetergent その他の各種試薬を添加しても混濁を防止できなかった。
(5) o-TB試薬中の氷酢酸, H₂O含量を75, 10% (o-Tは15%) にし, 15分加熱後流水中冷却させると呈色度は若干低目であるが, 呈色が安定化し, 同時に混濁を防止することができた。
(6) 検量線は直線性を示し, 測定精度は充分満足すべきものであった。
(7) 従来のo=TB試薬による血糖測定値とは広い濃度範囲にわたってほぼ完全に合致した成績を示した。
(8) この改良o-TB試薬は従来の試薬の欠点の一つを解決したものとして血糖検査には適切な方法であると思われる。

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This report presents a simple photometric method of serum albumin using buffered citrated solution of B C G.(pH4.0). Color development is stable and has a linearity with respect to albumin concentration over the range of 0 to 6.0g/dl. Precision in dupplicated and day-to-day measurments was good. Further studies with hyperbilirubinemic, lipemic and hemolytic sera showed no interference with this method. This procedure is simple and available for routine laboratory examination and also applicable to either manual or automatic analysis.

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The determination of serum glucose with o-toluidine using a glacial acetic acid medium is sensitive, precise and accurate enough for the requirements of a clinical laboratory. It is not convenient for a routine work because g acial acetic acid has irritant vapor.
Therefore, a new method of determination of serum glucose with o-toluidine using no glacial acetic acid (TGBM method) is presented. The procedure employs a mixture of glycollic acid, benzyl alcohol and methyl cellosolve instead of glacial acetic acid.
The new reagent (TGBM reagent) is non irritant, colorless, and stable for at least several months when stored at 4° in a brown bottle.
The coefficient of variation was ±2% by the TGBM method; recovery experiments gave a mean yield of 100%. Good agreement was observed between the TGBM method and the UV method using hexokinase-G-6 PDH or OTB method.

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