Age differences in cortisone induced changes of mitochondria, microbodies and endoplasmic reticulums of the mice hepatic cells and in the recovery process from them were studied electron microscopically and ultracytochemically, using female mice of SM strain. In case of cortisone treatment, the mitochondria decreased in number and increased in size. In the old aged, the changes were more markedly noticed but the recovery from them was slower compared with other younger groups. The cytochrome c oxidase activity was ultracytochemically demonstrated in the membrane system of the enlarged mitochondria in all age groups. After cortisone treatment, microbodies increased in number. In the old aged, the increase was more markedly noticed than other younger groups. Endoplasmic reticulums were decreased in number after cortisone treatment; in the old age group, the decrease was more marked and recovery from it was slower. Some interesting differences between mitochondria and microbodies in their changing pattern in case of cortisone treatment were discussed from various points of view.
Electrocardiographic abnormalities with advancing age were observed on 1937 men and 1794 women past 39 years of age, who visited the outpatient clinic of Department of Geriatrics, Hospital of Tokyo University. The tracings were classified according to the Minnesota Code. The subjects were devided into five groups by blood pressure on initial examination. Relationship between frequency of electrocardiographic abnormalities and blood pressure were studied in each decades for both sexes. The rate of abnormal Q was 1.5% in male and 0.4% in female. Left axis deviation (code 2-1) was more prevalent among males and its frequency increased with advancing age. There was no correlation between LAD and blood pressure. Prevalence of high amplitude R waves (code 3-1) were more common among males, but increase of rate with age was observed only among females. Frequency of high amplitude R waves and ST segment depression were associated with high blood pressure. Prevalence of ST segment depression was independent of serum cholesterol level. First degree of A-V block, right bundle branch block and premature beats among both sexes and atrial fibrillation among males increased in frequency with age and they were not associated with blood pressure. The relationship of left axis deviation, conduction defects and arrhythmia to latent coronary heart disease was discussed. It seemed unlikely that such non-specific changes frequently represent signs of asymptomatic coronary disease.
The present study was conducted to elucidate the effect of age on arterial blood gas and its Postural change in relation to obesity. The microanalysis of blood gas on capillary blood from ear lobe was proved to be valid in evaluating the arterial blood gas in the aged. While the PCO2 and pH in healthy subjects showed no variation with age, the regression of PO2 on age was significant with the regression line of PO2=106-0.41× age. Mean PO2 for 50-69 years of age was 79±4.8mmHg and for over 70 years 78±4.9mmHg. Both the young and the aged demonstrated slightly higher PO2 on sitting than on supine Position, but the difference between the two group was insignificant. In contrast to this, obese patients with their degree of obesity over 20% of standard weight showed a significant fall in PO2 with shift of Posture from sitting to supine. It is concluded that obesity has more notable effect than aging on Postural change in PO2.