It was reported that Mono Dansyl Cadaverine (MDC) inhibited ADP induced platelet aggregations and also inhibited Factor XIII, transgulutaminase, in plasma. We tried to investigate the effect of MDC on the erythrocyte deformability and blood coagulation system in this paper. In this study, we found that MDC inhibited platelet aggregations induced by collagen, noradrenalline, arachidonic acid and thrombin, and prolonged the euglobulin lysis time when it was added after forming of the fibrin clots. And we also found that MDC increase the osmotic fragility of erythrocyte. We supposed as these mechanisms that they were due to the inhibition of transglutaminase (Factor XIII) of erythrocyte membrane and plasm.
Platelet aggregation induced by thrombin was found to be specifically inhibited by water soluble substance extracted from Cortinellus Shiitake. Effective substance in this extract may be identified with Guanosine-5'-mono phosphate (GMP). This substance almost completely supressed blood platelet aggregation by thrombin in vitro, and it also inhibited ADP aggregation in vivo as same as in vitro. From our investigations for its various derivatives, it was hypothesized that both Guanosine and phosphate were necessary. Since the substance could inhibit aggregation without preincubation with the platelet, was able to speifically inhibit platelet aggragation by thrombin in vitro and could even prolong thrombin induced coagulation time, it was considered to have inhibiting effect through deactivation of thrombin by binding to thrombin and/or by supressing the thrombin receptor.
Ursodeoxycholic Acid (UDCA) 300mg/day was given to 31 aged subjects and 12 young subjects. The levels of serum lipids and serum bile acids were observed contunuatively for 4 weeks. We named the cases which showed decreases of Atherogenic Index (AI: Total cholesterol-HDL cholesterol/HDL cholesterol) 2 weeks after UDCA administration “Type UA”, and the others “Type UB”. The young subjects belonged mostly to “Type UA” (75%). On the other hand, the aged subjects who belonged to “Type UA” was 45.2%. In the aged subjects, 12 persons had cerebral infarction as past history. Ten of them belonged to “Type UB” and 2 of them belonged to “Type UA”. Serum bile acid was measured with gas liquid chromatography. In the “Type UA” group, the levels of chenodeoxycholic acid (CDCA) were increased significantly, but in “Type UB” group, they were not increased. There was significant negative correlation between the levels of serum CDCA and the levels of AI(R=0.609, p<0.05). The reasons why AT was decreased and CDCA in serum was increased in “Type UA” were not obvious. It is suggested that intestinal bacteria may play one of the important roles of bile acid metabolism.
Twenty three patients aged above 60 years who had been noticed hypertensive (systolic BP>160mmHg, diastolic BP>105mmHg) on admission were subjected in this study. In 13 patients of the subjects the blood pressure (BP) spontaneously lowered to normal range by 2 weeks after admission (NBP group), and the BP of another 10 patients still remained hypertensive at that time (HBP group). Assessments of the physical findings and hypertensive complications, and blood chemical and endocrinological examinations were made to compare the Pathophysiological conditions between the 2 groups. The mean age of the HBP group was slightly younger (p<0.05) than that of the NBP group. There were no differences in the obesity index and the general condition between the 2 groups. With regard to complications, complicating frequencies of diabetes mellitus, cerebrovascular troubles and hypertensive involvements of retina were somewhat greater in the HBP group compared with those in the NBP group, while there were no differences in terms of ECG change and the degree of cardiomegaly between the 2 groups. There were no apparent differences in the serum electrolyte levels, lipid levels and renal function between the 2 groups. In endocrinological examinations, the plasma renin activity and serum prolactin level tended to be increased in HBP group, and the plasma aldosterone concentration was significantly (p<0.05) greater than that in NBP group. There were no differences in the urinary catecholamine levels between the 2 groups. From these results, no apparent differences were observed in the blood pressure on admission and laboratory and physical examinations run in this study between the 2 groups except the points that the reninangiotensin system was slightly increased and complicating rates of retinal involvements and cerebrovascular troubles tended to be high in the HBP group compared with the NBP group. While there have to be many differences in pathophysiological condition and treatment between the 2 groups, it is considered to be difficult at the present time to differentiate one from the other in the 2 groups with means other than the observation of the transition of blood pressure.