To elucidate the mechanism of granulopoiesis in the aged, we studied granulo-macrophage (GM) colony formation in 13 healthy aged persons and 8 young adult volunteers.
The results obtained were as follows:
1. GM colony formation was observed when 2×10
5 marrow cells were treated by the addition of 10% human placental conditioned medium after 7 days culture. The aged group formed 113±19 colonies. Compared with 118±19 colonies formed in volunteer group, no significant difference could be found.
2. The concentration of colony stimulating factor (CSF) revealed no difinite influence to GM colony forming cells in both aged and volunteer group. However, to a same concentration of CSF, the aged group showed slightly depressed responsiveness.
3. Components of GM colony forming cells were differentiated by combined non-specific esterase staining.
In aged group, GM colony forming cells consisted of 91±3% naphthol AS-D chloroacetate esterase positive colonies (granulocyte colonies), 9±3% alpha-naphthyl acetate esterase positive colonies (monomacrophage colonies) and 4±3% both positive colonies (granulocyte-macrophage colonies), In volunteer group, there were 87±6%, 9±3% and 9±3% respectively. Again, no significant difference could be found.
From above findings, we assume that marrow function in the aged, compared with that of the young adults, was maintained normally with respect to colony formation and components of GM colony forming cells. On the contrary, it showed depressed responsiveness to CSF in the aged.
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