This report is the result of pyridinolcarbamate treatment of atherosclerosis of cholesterol-fed rabbits. 124 healthy albino male rabbits which had bred in our own farm and had the same age of 6 months, were kept on pellets contaning 1% cholesterol for 15 weeks for the production of atherosclerotic rabbits. At the end of this period, cholesterol pellets were taken off and they were returned to a normal diet. These 124 atheromatous rabbits were divided into two groups (Placebo control group and pyridinolcarbamate group). The placebo control group received gelatin capsule containing potato starch as a placebo, and the treated group recieved gelatin capsule containing pyridinolcarbamate in a daily dose of 10-20-30mg/kg by mouth. Each same number of animals (5 or 7) of both groups were sacrificed on the 4th, 7th day, 3rd, 6th, 10th, 20th and 30th week of the treatment under normal diet. The aorta was examined histologically and biochemically under the double blind technique.
Results: During this experiment, there was no statistically significant difference between the placebo control and pyridinolcarbamate group in daily food consumption, body weight and serum total cholesterol level.
Histological observations: In the placebo control group, an abundant accumulation of fatty substances and a scantiness of regressive changes were characteristic and these features still remained even 10-20 weeks after the withdrawal of cholesterol. Thirty weeks after the withdrawal of cholesterol, fibrous caps were produced covering atheromatous masses, but there are abundant sudanophilic substances in the central portion of the atheromatous lesions covered by the fibrous cap.
In treated group, the rapid disappearance of edema and the appearance of smooth muscle fibers inside the atheromatous lesion was characteristic in the short term of pyridinolcarbamate treatment and after the 10 weeks of pyridin olcarbamate treatment atheromatous lesions were almost completely replaced with the regenerated smooth muscle fibers.
Biochemical measurement of cholesterol content of arterial walls: In rabbits treated by pyridinolcarbamate more than 6 weeks the cholesterol content of aortic wall was significantly lowered in the pyridinolcarbamate group than placebo control group (
P<0.05). There was found no apparent toxic effect in the biological and histological analysis in animals received pyridinolcarbamate.
The administration of pyridinolcarbamate in a daily dose of 10 to 30mg has been shown to promote significantly the naturally occuring healing in atheromatous lesions of cholesterol-fed rabbits and the replacement of atheromatous masses with regenerated smooth muscle fibres has been noted as a highly important finding in pyridinolcarbamate treatment of atheromatous lesions.
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