The purpose of investigation isto determine the effect of the anabolic steroid upon total serum cholesterol level. This study was done with the double blind method in 94 atherosclerotic patients who were hospitalised in the Koseiso Sanatorium. These patients were divided into 2 groups, and one received 1.5/mg/day of the anabolic steroid and the other received its placebo during a period of 1 year. The results of this study was clearly demonstrated that total serum cholesterol level in the group receiving the anabolic steroid became greatly depressed within 1 month after the administration, and was kept to be low throughout the period of administration. Twenty-nine out of 46 cases showed the cholesterol level to be 20% less than the original level. The difference between the 2 groups in the cholesterol level was significant statistically with a risk of 5%. The cholesterol level returned to the original after the discontinuation of the drug. In the group receiving the anabolic steroid, S-GOT and S-GPT showed increase of more than 40 units in 15 out of 46 cases, but they were within normal limits in the rest of the cases. S-LDH, icterus index, ZTT and serum alkaline phosphatase activity remained unchanged in all cases throughout this study. Thus, it is concluded that total serum cholesterol level is significantly decreased during the administration of the anabolic steroid. Upon a long-term administration of the drug, however, the patients should be followed up carefully as to the occurrence of side effects.
In the previous paper, the authors showed the influence of aging on the nucleic acid metabolism induced by experimental burn in male mice of DDY strain. Estimating from results of the determination of nucleic acid concentration and 32P incorporation in-vivo, the increaced turn-over of nucleic acid metabolism in response to surgical stress appears to be less pronounced in liver tissue of aged animals than in the younger. However, to get any speculation about the nucleic acid metabolism in human liver, it might be necessary to get some informations cocerning the age-related changes of the concentration of nucleic acids and the related enzymes in human liver. Therefore, the authors have determined quantitatively the concentrations of protein-nitrogen, nucleic acids (DNA and RNA) and the related enzymes of liver tissue in various age. Liver specimen was obtained during laparotomy in 14 cases (3 cases with gastric ulcer, 2 cases with cholelithiassi and 10 cases with non-advanced gastric cancer). The range of age in years was from 24 through 76, and none of all cases showed any hepatic disorder or imparied hepatic function. No clear change was found in the concentration of protein-N, DNA, alkaline ribonuclease and alkaline phosphomonoesterase between patients of the 3rd and 8th decades, and on the other hand, the concentration of RNA, acid ribonuclease, and acid phosphomonoesterase per wet weight were decreased in livers of patients above the 7th decade. The RNA: DNA ratio seemed to be, also, decerased in livers of aged patients. The results could not mean the exact age-related change of nucleic acids in healthy human because the authors could not definitely exclude the influence of the malignant or benign lesion on the concentrations of nucleic acids or enzymes in the data described above. However, some age-related changes might be, possibly, reflected to the differences in the concentrations of nucleic acids and enzymes among those patients.
So far, the assessment of aging process has been studied in various methods. As one of them, Hinohara devised a simple one assessing of balance ability named “Single-foot standing test with eyes closed”. We write down its method and the result as follows. THE METHOD: To make the exminee stand up on one foot, to make him close his eyes and to time how long can he keep standing balanced. To try the same test to both feet alternately and to count out the average time by second. The average time of ten seconds do not require further test. THE EXAMINEE: The number of the examinees is 756 (M: 523, F: 233) and they are apparently healthy persons of third to nineth decades, and who are living ordinary social lives. THE RESULT: For both sexes over 40-year-old, the time of “Single-foot standing test with eyes closed” gets shorter with aging and it does not depend on neither the height nor the sport of the examinee. According to the “Single-foot standing test with eyes closed”, the balance ability of over 40-year-old persons descend with aging and the descending rate for women is less than that for men. From the above, our test can be recognized as one method assessing the aging process and the result told us that women is slower of aging than men.
Three hypercholesterolemic patients (Type 3) were subjected to receive carbohydrate rich diet (as sucrose or starch) for the purpose of analyzing the effect of the kinds of carbohydrate on the plasma lipids. During the cource in which patients were administered with sucrose rich diet, triglyceride concentration in the plasma increased more remarkably, and palmitic acid as well as oleic acid which can be both synthesized in the body increased, and linoleic acid decreased. Dogs' experiments were carried out to evaluate whether glucose or fructose which can be split from either starch or sucrose would be responsible for the increase of plasma lipids. The group administered with fructose rich diet showed higher triglyceride concentration in the plasma and in the liver. This group also showed the increased palmitic and oleic acid, and decreased linoleic acid in the triglyceride fraction of the liver. These results indicate that fructose might be more responsible than glucose for the increment of the plasma lipids by way of converting itself into lipids.