It has been assumed that vitamin E suppressed an increase of superoxides in tissues and organs in aging process. The authors intended to clarify the relationship between the aging of “fluorescent granular perithelium (F.G.P.)” and vitamin E in this study. By the authors' investigation, the granular perithelial cells were distributed in cerebral fine vessels, especially, in bifurcating regions of them, and contained large fluorescent inclusion bodies. The F.G.P. changed in shape and contents in aging process. In order to investigate the effect of vitamin E on F.G.P., two groups of rats fed with vitamin E deficient or vitamin E rich (Juvela-nicotinate) chow were studied at light and electron microscopical levels. According to the previous reports of the authors, it had be ascertained that the granular perithelial cells could incorporate efficiently exogenous substances (horseradish peroxidase, trypan blue and ferritin), if administered intraventricularly, and were facilitated in the uptake capacity with administration of vasopressin. Subsequently, the F.G.P. were interpreted as a kind of scavenger cells in a central nervous tissue. If they were fallen into a degeneration, waste products in a central nervous tissue would be piled up without any degradation. In this study, the perithelial cells in rats which were fed with vitamin E deficient chow for eight to ten months tended to take a regressive change. That is, in general, the contrast in electron opacity of cytoplasmic organelles of F.G.P. became low and especially, limiting membranes of intracellular granules became obscure. Concomitantly, mitochondria were swollen and their cristae were arranged irregularly. Histochemically, acid phosphatase activity was hsgh and was detected through the cytoplasm. Occasionally, degenerating F.G.P. with dark matrices appeared between endothelium and nervous tissues. Further, it was also noticeable that after the degeneration of perithelial cells, collagen fibers came out in the intercellular spaces between degenerating perithelial cells and nervous tissue. On the contrary, the perithelial cells in rats which were fed with the chow containing Juvela-nicotinate did not reveal distinct regressive signs but some exceptions. Intracellular granules changed in profiles and contents, and occasionally took honeycomb forms, but the other signs for regressive changes did not appear in cytoplasmic organelles. Therefore, the perithelial cells in rats fed with vitamin E deficient chow for a long period tended to go down in their function, and the regression could induce a disorder of an uptake capacity for metabolic wastes in a central nervous tissue, and enhance a fibrosis around cerebral fine vessels. In other words, it could be said that vitamin E was useful for maintaining a function and morphology of the granular perithelial cells in aging process.
We have already reported, not only, several cases with myocardial infarction which were caused by some kinds of disturbance in gall bladder (G.B.), but also, some ones with arrythmias and/or angina pectoris; of course, they have been suffering from coronary sclerosis. In this paper, the authors want to represent high incidence of complication with myocardial infarction (MI) and G.B. disturbance and the possibility which G.B. disfunction would contribute the patients with ishemic heart diseases progress to MI. Fourty seven cases in sixty three with MI, 74.9% of the patients, including six cases of autopsies, showed abnormalities on oral cholecystographic examination. Thirteen cases (20.3%) had gall stones, and the other cases showed abnormalities of morphology and/or disfunction of G.B. contraction. As for gall stone, statistical difference revealed high frequency appearance in female than in male, but concerning comparison with the position of MI, and/or age, no difference were showed. High incidence of contraction abnormalities revealed in the cases with atypical MI. than typical MI. which were classified by clinical and laboratory findings, though no difference of serum cholesterol and triglycerides levels between the patients with abnormal oral cholecystography and normal ones. This investigation revealed the possibility which G.B. disturbance would contribute the patients with ishemic heart diseases developed to atypical MI, but it is seldom to be seen the possibility which disturbance of G.B. should be a factor of coronary sclerosis.