The first symptoms of this disease appear on the leaf of teosinte (Euchlaena mexicana SCHRAD.) as small water-soaked dots, which gradually grow into oval diseased spots, gray in the interior, and brown in the margin. They, then, continue to get larger to stripes, the interior of which shows dirty white or light brown color, the margin being purplish brown or dark brown. The length of the stripe ranges from several milimeters to half of one leaf-length. This disease can be observed during the months from July to October, but in July and August it is most severe. As it has been found from most parts of Japan, it can be believed as a common disease of teosinte. The pathogen under consideration resembles Pseudomonas andropogonis in bacteriological characters, except that its colony is not viscid but butyrous and that it reduces nitrates. Spray inoculation of the isolate causes damage on Sudangrass and corn and the symptoms are the same as those by P. andropogonis on the above same plants. Therefore, the author has identified it as P. andropogonis (E. F. SMITH) STAPP, considering its bacteriological and parasitic characters. And it is the first time that P. andropogonis has been found to attack teosinte, so he has named the disease "bacterial stripe of teosinte". The bacteriological characters of the pathogen are as follows. It is a rod of 0.5×0.9 to 2.9μ in size, with 1 to 3 monopolar flagella and a few fimbriae. It is aerobic, Gram-negative and without capsules. On agar, the colonies are grayish-white, round, convex, smooth, glistening and butyrous. Green fluorescent pigment is not produced in. KING, WARD and RANEy agar. Gelatin is not liquefied. Milk is slowly peptonized, and both litmus and nitrates are reduced. Neither indole nor hydrogen sulphide develops, but ammonia is formed. Acid but no gas is formed from xylose, glucose, laevulose, galactose, mannose, glycerol, mannitol and sorbitol, and by certain strains, from rhamnose also in AYERS, JOHNSON agar with BTB as indicator ; neither acid nor gas from lactose, saccharose, maltose, raffinose, starch, inulin or dextrine. Starch is hydrolysed. There is slight growth in broth containing 4% sodium chloride. The optimum growth temperature is 35℃, its minimum below 10℃ ; the thermal death-points is at 52℃.
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