The use of cattle slurry has been associated with weedseed invasion of farmland. Accordingly, a study was conducted to determine the temperatures and retention times lethal to weed seeds in cattle slurry. Seeds of six alien weed species (Solanum carolinense L., S. amencanum Mill., Abutilon theophrasti Medic., Phytolacca americana L., Amaranthus spinosus L., and A. patulus Bertoloni) were heated for up to 9 days in deionized water and cattle slurry, respectively. In deionized water, almost all seeds lost viability after one day at 55℃. Seed viability in response to temperatures of 35 and 45℃ varied with species. The species tested were divided into three groups-namely, 1) species in respect of which viability was barely reduced at both temperatures (S. carolinense, and A. spinosus), 2) species in respect of which viability was barely reduced at 35℃, and in respect of which viability decreased as retention time increased at 45℃ (S. americanum, and A. patulus), and 3) species in respect of which viability decreased as retention time increased at both temperatures (A. theophrasti, and P. americana). In cattle slurry, almost all seeds lost viability after one day at 50℃. At 45℃, S. carolinense seeds retained viability for up to five days, and seeds of Amaranthus spp. retained viability for two days, while seed viability of the other three species was lost after one day. The viability of S. carolinense seeds was not reduced after they were held at 35℃ for nine days; however, some decrease in viability was detected at 40℃. At both temperatures, seed viability of Amaranthus spp. decreased as retention time increased ; however, viability had not been reduced to zero after nine days. Seed viability of S. americanum, A. theophrasti and P. americana was almost completely lost in five and two days at 35 and 40℃, respectively. For S. carolinense, which was most tolerant to heat in this study, a logistic regression equation was calculated between retention time and seed viability in cattle slurry in 45℃. A 95% confidence interval for the retention time at which viability was reduced to 10% was estimated as 7.0-8.4 days. From these findings, it is recommended that the retention times of cattle slurry should be longer than one and nine days at 50 and 45℃, respectively, in order to kill all weed seeds.
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