In order to obtain some information on the mechanism controlling the accumulation of 5'-nucleotides in Shii-take, fruit body of
Lentinus edodes, during the cooking, examinations were undertaken on the effect of drying condition on the activities of nuclease and phosphomonoesterase of Shii-take, and on the effect of heating-rate on the accumulation of 5'-nucleotides in Shii-take.
1) In an examination on the heat stability of nuclease and phosphomonoesterase, it was found that nuclease was more labile on heating than phosphomonoesterase in raw Shii-take, but became more stable with the decrease of water content, especially below 30 %, during drying.
2) On the forced-draft drying of Shii-take at 15, 50, or 80°C, no reduction was found in enzymatic activities during drying, except the reduction of phosphomonoesterase activity in the Shii-take dried at 80 °C.
3) On the cooking test at various heating-rates of dried Shii-take which was soaked in water at 0 °C, the highest quantity of 5'-nucleotides was found in the Shii-take cooked at a heating-rate of 7 °C/min, and the Shii-take dried at 80 °C proved to accumulate higher quantity of 5'-nucleotides during the cooking than that dried at 50 °C.
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